A novel ratiometric fluorescent aptasensor accurately detects patulin contamination in fruits and fruits products

Patulin (PAT) contamination in fruit and fruit products is a significant public health concern. Here, we developed a ratiometric fluorescent aptasensor for PAT detection based on aptamer-recognition and Exonuclease III amplification. Two structure selective dyes, SYBR Green I (SGI) and N-methyl meso...

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Autores principales:	Jiankang Deng, Jing Hu, Juanjuan Zhao, Zhiqiang Zhang, Qinghua Wang, Rongrong Wu
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2022
Materias:	Patulin Aptamer Exonuclease III SYBR Green I N-methyl mesoporphyrin IX Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/68de987568454ea1b39187880681df52
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spelling	oai:doaj.org-article:68de987568454ea1b39187880681df522021-12-04T04:33:29ZA novel ratiometric fluorescent aptasensor accurately detects patulin contamination in fruits and fruits products1878-535210.1016/j.arabjc.2021.103569https://doaj.org/article/68de987568454ea1b39187880681df522022-02-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1878535221005840https://doaj.org/toc/1878-5352Patulin (PAT) contamination in fruit and fruit products is a significant public health concern. Here, we developed a ratiometric fluorescent aptasensor for PAT detection based on aptamer-recognition and Exonuclease III amplification. Two structure selective dyes, SYBR Green I (SGI) and N-methyl mesoporphyrin IX (NMM), were used as fluorescent probes. In the developed biosensing system, the binding of PAT to aptamer triggered the liberation of cDNA. Subsequently, amplification was mediated by Exonuclease III. S1 was released from the S1-S2 duplex by enzymatic hydrolyzation and incorporated into a stable G-quadruplex. As a result, the fluorescence of SGI decreased, whereas that of NMM increased. There was a strong linear correlation between the relative fluorescence intensity and PAT concentrations (20 to 500 ng·L−1 range) (R2 = 0.99). The biosensing system was highly sensitive, and could detect PAT concentration as low as 4.7 ng·L−1. The sensor was also highly specific, and could differentiate PAT from several other related mycotoxins. In summary, we developed a new bioassay for the accurate detection of PAT contamination in fruits and fruit products. This research provides a new approach for developing ratiometric bioassays based on structure-selective dyes and enzymatic conversion processes.Jiankang DengJing HuJuanjuan ZhaoZhiqiang ZhangQinghua WangRongrong WuElsevierarticlePatulinAptamerExonuclease IIISYBR Green IN-methyl mesoporphyrin IXChemistryQD1-999ENArabian Journal of Chemistry, Vol 15, Iss 2, Pp 103569- (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	Patulin Aptamer Exonuclease III SYBR Green I N-methyl mesoporphyrin IX Chemistry QD1-999
spellingShingle	Patulin Aptamer Exonuclease III SYBR Green I N-methyl mesoporphyrin IX Chemistry QD1-999 Jiankang Deng Jing Hu Juanjuan Zhao Zhiqiang Zhang Qinghua Wang Rongrong Wu A novel ratiometric fluorescent aptasensor accurately detects patulin contamination in fruits and fruits products
description	Patulin (PAT) contamination in fruit and fruit products is a significant public health concern. Here, we developed a ratiometric fluorescent aptasensor for PAT detection based on aptamer-recognition and Exonuclease III amplification. Two structure selective dyes, SYBR Green I (SGI) and N-methyl mesoporphyrin IX (NMM), were used as fluorescent probes. In the developed biosensing system, the binding of PAT to aptamer triggered the liberation of cDNA. Subsequently, amplification was mediated by Exonuclease III. S1 was released from the S1-S2 duplex by enzymatic hydrolyzation and incorporated into a stable G-quadruplex. As a result, the fluorescence of SGI decreased, whereas that of NMM increased. There was a strong linear correlation between the relative fluorescence intensity and PAT concentrations (20 to 500 ng·L−1 range) (R2 = 0.99). The biosensing system was highly sensitive, and could detect PAT concentration as low as 4.7 ng·L−1. The sensor was also highly specific, and could differentiate PAT from several other related mycotoxins. In summary, we developed a new bioassay for the accurate detection of PAT contamination in fruits and fruit products. This research provides a new approach for developing ratiometric bioassays based on structure-selective dyes and enzymatic conversion processes.
format	article
author	Jiankang Deng Jing Hu Juanjuan Zhao Zhiqiang Zhang Qinghua Wang Rongrong Wu
author_facet	Jiankang Deng Jing Hu Juanjuan Zhao Zhiqiang Zhang Qinghua Wang Rongrong Wu
author_sort	Jiankang Deng
title	A novel ratiometric fluorescent aptasensor accurately detects patulin contamination in fruits and fruits products
title_short	A novel ratiometric fluorescent aptasensor accurately detects patulin contamination in fruits and fruits products
title_full	A novel ratiometric fluorescent aptasensor accurately detects patulin contamination in fruits and fruits products
title_fullStr	A novel ratiometric fluorescent aptasensor accurately detects patulin contamination in fruits and fruits products
title_full_unstemmed	A novel ratiometric fluorescent aptasensor accurately detects patulin contamination in fruits and fruits products
title_sort	novel ratiometric fluorescent aptasensor accurately detects patulin contamination in fruits and fruits products
publisher	Elsevier
publishDate	2022
url	https://doaj.org/article/68de987568454ea1b39187880681df52
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A novel ratiometric fluorescent aptasensor accurately detects patulin contamination in fruits and fruits products

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