Characterization, Cytotoxicity and Anti-Inflammatory Effect Evaluation of Nanocapsules Containing Nicotine

(1) Background: Nanotechnology is an emerging field that can be applied in the biomedical area. In this study, Eudragit nanocapsules (NCs) containing nicotine were produced. Nicotine is the main alkaloid found in tobacco and has anti-inflammatory properties. NCs containing nicotine may be used as an...

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Autores principales:	Carolina Landau Albrecht, Laura Elena Sperling, Daikelly Iglesias Braghirolli, Patricia Pranke
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	nanocapsules Eudragit nicotine inflammation PC12 Technology T Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/68e5f1eb34c04d568e9b32526ae78372
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spelling	oai:doaj.org-article:68e5f1eb34c04d568e9b32526ae783722021-11-25T16:46:33ZCharacterization, Cytotoxicity and Anti-Inflammatory Effect Evaluation of Nanocapsules Containing Nicotine10.3390/bioengineering81101722306-5354https://doaj.org/article/68e5f1eb34c04d568e9b32526ae783722021-11-01T00:00:00Zhttps://www.mdpi.com/2306-5354/8/11/172https://doaj.org/toc/2306-5354(1) Background: Nanotechnology is an emerging field that can be applied in the biomedical area. In this study, Eudragit nanocapsules (NCs) containing nicotine were produced. Nicotine is the main alkaloid found in tobacco and has anti-inflammatory properties. NCs containing nicotine may be used as an adjuvant therapy in the treatment of inflammation in the central nervous system. (2) Methods: Nanocapsules were prepared by the interfacial deposition of the pre-formed polymer method and characterized in terms of zeta potential, diameter, polydispersity index, pH, encapsulation efficiency (EE), stability and sustained release profile. In vitro tests with the PC12 cell line were performed, such as MTT, LIVE/DEAD and ELISA assays, to verify their cytotoxic and anti-inflammatory effects. (3) Results: The nanocapsules presented satisfactory values of the characterization parameters; however, poor encapsulation was obtained for nicotine (8.17% ± 0.47). The in vitro tests showed that the treatment with nanocapsules reduced cell viability, which suggests that the Eudragit or the amount of polymer on top of the cells may be detrimental to them, as the cells were able to survive when treated with bulk nicotine. ELISA showed an increment in the expression of IL-6 and IL-1β, corroborating the hypothesis that NCs were toxic to the cells because of the increase in the levels of these pro-inflammatory cytokines. (4) Conclusions: This study demonstrates that NCs of Eudragit present toxicity. It is therefore necessary to improve NC formulation to obtain better values for the encapsulation efficiency and reduce toxicity of these nanodevices.Carolina Landau AlbrechtLaura Elena SperlingDaikelly Iglesias BraghirolliPatricia PrankeMDPI AGarticlenanocapsulesEudragitnicotineinflammationPC12TechnologyTBiology (General)QH301-705.5ENBioengineering, Vol 8, Iss 172, p 172 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	nanocapsules Eudragit nicotine inflammation PC12 Technology T Biology (General) QH301-705.5
spellingShingle	nanocapsules Eudragit nicotine inflammation PC12 Technology T Biology (General) QH301-705.5 Carolina Landau Albrecht Laura Elena Sperling Daikelly Iglesias Braghirolli Patricia Pranke Characterization, Cytotoxicity and Anti-Inflammatory Effect Evaluation of Nanocapsules Containing Nicotine
description	(1) Background: Nanotechnology is an emerging field that can be applied in the biomedical area. In this study, Eudragit nanocapsules (NCs) containing nicotine were produced. Nicotine is the main alkaloid found in tobacco and has anti-inflammatory properties. NCs containing nicotine may be used as an adjuvant therapy in the treatment of inflammation in the central nervous system. (2) Methods: Nanocapsules were prepared by the interfacial deposition of the pre-formed polymer method and characterized in terms of zeta potential, diameter, polydispersity index, pH, encapsulation efficiency (EE), stability and sustained release profile. In vitro tests with the PC12 cell line were performed, such as MTT, LIVE/DEAD and ELISA assays, to verify their cytotoxic and anti-inflammatory effects. (3) Results: The nanocapsules presented satisfactory values of the characterization parameters; however, poor encapsulation was obtained for nicotine (8.17% ± 0.47). The in vitro tests showed that the treatment with nanocapsules reduced cell viability, which suggests that the Eudragit or the amount of polymer on top of the cells may be detrimental to them, as the cells were able to survive when treated with bulk nicotine. ELISA showed an increment in the expression of IL-6 and IL-1β, corroborating the hypothesis that NCs were toxic to the cells because of the increase in the levels of these pro-inflammatory cytokines. (4) Conclusions: This study demonstrates that NCs of Eudragit present toxicity. It is therefore necessary to improve NC formulation to obtain better values for the encapsulation efficiency and reduce toxicity of these nanodevices.
format	article
author	Carolina Landau Albrecht Laura Elena Sperling Daikelly Iglesias Braghirolli Patricia Pranke
author_facet	Carolina Landau Albrecht Laura Elena Sperling Daikelly Iglesias Braghirolli Patricia Pranke
author_sort	Carolina Landau Albrecht
title	Characterization, Cytotoxicity and Anti-Inflammatory Effect Evaluation of Nanocapsules Containing Nicotine
title_short	Characterization, Cytotoxicity and Anti-Inflammatory Effect Evaluation of Nanocapsules Containing Nicotine
title_full	Characterization, Cytotoxicity and Anti-Inflammatory Effect Evaluation of Nanocapsules Containing Nicotine
title_fullStr	Characterization, Cytotoxicity and Anti-Inflammatory Effect Evaluation of Nanocapsules Containing Nicotine
title_full_unstemmed	Characterization, Cytotoxicity and Anti-Inflammatory Effect Evaluation of Nanocapsules Containing Nicotine
title_sort	characterization, cytotoxicity and anti-inflammatory effect evaluation of nanocapsules containing nicotine
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/68e5f1eb34c04d568e9b32526ae78372
work_keys_str_mv	AT carolinalandaualbrecht characterizationcytotoxicityandantiinflammatoryeffectevaluationofnanocapsulescontainingnicotine AT lauraelenasperling characterizationcytotoxicityandantiinflammatoryeffectevaluationofnanocapsulescontainingnicotine AT daikellyiglesiasbraghirolli characterizationcytotoxicityandantiinflammatoryeffectevaluationofnanocapsulescontainingnicotine AT patriciapranke characterizationcytotoxicityandantiinflammatoryeffectevaluationofnanocapsulescontainingnicotine
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Characterization, Cytotoxicity and Anti-Inflammatory Effect Evaluation of Nanocapsules Containing Nicotine

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