A new murine esophageal organoid culture method and organoid-based model of esophageal squamous cell neoplasia
Summary: Organoids mimic the physiologic and pathologic events of organs. However, no consensus on esophageal organoid (EO) culture methods has been reached. Moreover, organoid models reproducing esophageal squamous cell carcinoma (ESCC) initiation have been unavailable. Herein, we sought to develop...
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2021
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oai:doaj.org-article:68eefaa00f0d4a1d8e3b99253eb5f3472021-11-28T04:36:25ZA new murine esophageal organoid culture method and organoid-based model of esophageal squamous cell neoplasia2589-004210.1016/j.isci.2021.103440https://doaj.org/article/68eefaa00f0d4a1d8e3b99253eb5f3472021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2589004221014115https://doaj.org/toc/2589-0042Summary: Organoids mimic the physiologic and pathologic events of organs. However, no consensus on esophageal organoid (EO) culture methods has been reached. Moreover, organoid models reproducing esophageal squamous cell carcinoma (ESCC) initiation have been unavailable. Herein, we sought to develop an esophageal minimum essential organoid culture medium (E-MEOM) for culturing murine EOs and establishing an early ESCC model. We formulated E-MEOM to grow EOs from a single cell with clonal expansion, maintenance, and passage. We found that EOs cultured in E-MEOM were equivalent to the esophageal epithelium by histological analysis and transcriptomic study. Trp53 knockout and KrasG12D expression in EOs induced the development of esophageal squamous neoplasia, an early lesion of ESCC. Here we propose the new formula for EO culture with minimum components and the organoid model recapitulating ESCC initiation, laying the foundation for ESCC research and drug discovery.Biyun ZhengKyung-Pil KoXuefen FangXiaozhong WangJie ZhangSohee JunBong-Jun KimWenyi LuoMoon Jong KimYoun-Sang JungChristopher L. CervantesJae-Il ParkElsevierarticleCancerBiological sciences research methodologiesMethodology in biological scienceScienceQENiScience, Vol 24, Iss 12, Pp 103440- (2021) |
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DOAJ |
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DOAJ |
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Cancer Biological sciences research methodologies Methodology in biological science Science Q |
| spellingShingle |
Cancer Biological sciences research methodologies Methodology in biological science Science Q Biyun Zheng Kyung-Pil Ko Xuefen Fang Xiaozhong Wang Jie Zhang Sohee Jun Bong-Jun Kim Wenyi Luo Moon Jong Kim Youn-Sang Jung Christopher L. Cervantes Jae-Il Park A new murine esophageal organoid culture method and organoid-based model of esophageal squamous cell neoplasia |
| description |
Summary: Organoids mimic the physiologic and pathologic events of organs. However, no consensus on esophageal organoid (EO) culture methods has been reached. Moreover, organoid models reproducing esophageal squamous cell carcinoma (ESCC) initiation have been unavailable. Herein, we sought to develop an esophageal minimum essential organoid culture medium (E-MEOM) for culturing murine EOs and establishing an early ESCC model. We formulated E-MEOM to grow EOs from a single cell with clonal expansion, maintenance, and passage. We found that EOs cultured in E-MEOM were equivalent to the esophageal epithelium by histological analysis and transcriptomic study. Trp53 knockout and KrasG12D expression in EOs induced the development of esophageal squamous neoplasia, an early lesion of ESCC. Here we propose the new formula for EO culture with minimum components and the organoid model recapitulating ESCC initiation, laying the foundation for ESCC research and drug discovery. |
| format |
article |
| author |
Biyun Zheng Kyung-Pil Ko Xuefen Fang Xiaozhong Wang Jie Zhang Sohee Jun Bong-Jun Kim Wenyi Luo Moon Jong Kim Youn-Sang Jung Christopher L. Cervantes Jae-Il Park |
| author_facet |
Biyun Zheng Kyung-Pil Ko Xuefen Fang Xiaozhong Wang Jie Zhang Sohee Jun Bong-Jun Kim Wenyi Luo Moon Jong Kim Youn-Sang Jung Christopher L. Cervantes Jae-Il Park |
| author_sort |
Biyun Zheng |
| title |
A new murine esophageal organoid culture method and organoid-based model of esophageal squamous cell neoplasia |
| title_short |
A new murine esophageal organoid culture method and organoid-based model of esophageal squamous cell neoplasia |
| title_full |
A new murine esophageal organoid culture method and organoid-based model of esophageal squamous cell neoplasia |
| title_fullStr |
A new murine esophageal organoid culture method and organoid-based model of esophageal squamous cell neoplasia |
| title_full_unstemmed |
A new murine esophageal organoid culture method and organoid-based model of esophageal squamous cell neoplasia |
| title_sort |
new murine esophageal organoid culture method and organoid-based model of esophageal squamous cell neoplasia |
| publisher |
Elsevier |
| publishDate |
2021 |
| url |
https://doaj.org/article/68eefaa00f0d4a1d8e3b99253eb5f347 |
| work_keys_str_mv |
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