Periodontal Pathogens Inhabit Root Caries Lesions Extending beyond the Gingival Margin: A Next-Generation Sequencing Analysis

We performed a comprehensive microbiome analysis of root caries lesions using 22 teeth extracted from patients with severe periodontitis. The carious lesions were mechanically collected and cryo-pulverized following tooth extraction. Differences in the microbiome were compared between independent le...

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Autores principales:	Shoji Takenaka, Naoki Edanami, Yasutaka Komatsu, Ryoko Nagata, Traithawit Naksagoon, Maki Sotozono, Takako Ida, Yuichiro Noiri
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	root caries oral microbiota active lesion MiSeq amplicon sequencing Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/68f422e2e8b34d33b4f8e7f761330308
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spelling	oai:doaj.org-article:68f422e2e8b34d33b4f8e7f7613303082021-11-25T18:25:20ZPeriodontal Pathogens Inhabit Root Caries Lesions Extending beyond the Gingival Margin: A Next-Generation Sequencing Analysis10.3390/microorganisms91123492076-2607https://doaj.org/article/68f422e2e8b34d33b4f8e7f7613303082021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2349https://doaj.org/toc/2076-2607We performed a comprehensive microbiome analysis of root caries lesions using 22 teeth extracted from patients with severe periodontitis. The carious lesions were mechanically collected and cryo-pulverized following tooth extraction. Differences in the microbiome were compared between independent lesions at the supragingival site (SG) and lesions extending beyond the gingival margin (GCB). DNA was extracted and the microbiome was characterized on the basis of the V3-V4 hypervariable region of the 16S rRNA gene using paired-end sequencing on an Illumina MiSeq device. The microbiota in root caries lesions showed compositionally distinct microbiota depending on the location. The most abundant OTUs in the SG group were <i>Streptococcus</i> (26.0%), <i>Actinomyces</i> (10.6%), and <i>Prevotella</i> (7.6%). GCB presented <i>Prevotella</i> (11.1%) as the most abundant genus, followed by <i>Fusobacterium</i> (9.6%) and <i>Actinomyces</i> (8.7%). The SG group showed a lack of uniformity in microbiota compared with the GCB group. The bacterial profiles of GCB varied considerably among patients, including periodontal pathogens such as <i>Porphyromonas</i>, <i>Selenomonas</i>, <i>Filifactor</i>, <i>Peptococcus</i>, and <i>Tannerella</i>. Periodontal pathogens inhabit root caries lesions that extend beyond the gingival margin. This study provides a new perspective for elucidating the microbial etiology of root caries.Shoji TakenakaNaoki EdanamiYasutaka KomatsuRyoko NagataTraithawit NaksagoonMaki SotozonoTakako IdaYuichiro NoiriMDPI AGarticleroot cariesoral microbiotaactive lesionMiSeq amplicon sequencingBiology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2349, p 2349 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	root caries oral microbiota active lesion MiSeq amplicon sequencing Biology (General) QH301-705.5
spellingShingle	root caries oral microbiota active lesion MiSeq amplicon sequencing Biology (General) QH301-705.5 Shoji Takenaka Naoki Edanami Yasutaka Komatsu Ryoko Nagata Traithawit Naksagoon Maki Sotozono Takako Ida Yuichiro Noiri Periodontal Pathogens Inhabit Root Caries Lesions Extending beyond the Gingival Margin: A Next-Generation Sequencing Analysis
description	We performed a comprehensive microbiome analysis of root caries lesions using 22 teeth extracted from patients with severe periodontitis. The carious lesions were mechanically collected and cryo-pulverized following tooth extraction. Differences in the microbiome were compared between independent lesions at the supragingival site (SG) and lesions extending beyond the gingival margin (GCB). DNA was extracted and the microbiome was characterized on the basis of the V3-V4 hypervariable region of the 16S rRNA gene using paired-end sequencing on an Illumina MiSeq device. The microbiota in root caries lesions showed compositionally distinct microbiota depending on the location. The most abundant OTUs in the SG group were <i>Streptococcus</i> (26.0%), <i>Actinomyces</i> (10.6%), and <i>Prevotella</i> (7.6%). GCB presented <i>Prevotella</i> (11.1%) as the most abundant genus, followed by <i>Fusobacterium</i> (9.6%) and <i>Actinomyces</i> (8.7%). The SG group showed a lack of uniformity in microbiota compared with the GCB group. The bacterial profiles of GCB varied considerably among patients, including periodontal pathogens such as <i>Porphyromonas</i>, <i>Selenomonas</i>, <i>Filifactor</i>, <i>Peptococcus</i>, and <i>Tannerella</i>. Periodontal pathogens inhabit root caries lesions that extend beyond the gingival margin. This study provides a new perspective for elucidating the microbial etiology of root caries.
format	article
author	Shoji Takenaka Naoki Edanami Yasutaka Komatsu Ryoko Nagata Traithawit Naksagoon Maki Sotozono Takako Ida Yuichiro Noiri
author_facet	Shoji Takenaka Naoki Edanami Yasutaka Komatsu Ryoko Nagata Traithawit Naksagoon Maki Sotozono Takako Ida Yuichiro Noiri
author_sort	Shoji Takenaka
title	Periodontal Pathogens Inhabit Root Caries Lesions Extending beyond the Gingival Margin: A Next-Generation Sequencing Analysis
title_short	Periodontal Pathogens Inhabit Root Caries Lesions Extending beyond the Gingival Margin: A Next-Generation Sequencing Analysis
title_full	Periodontal Pathogens Inhabit Root Caries Lesions Extending beyond the Gingival Margin: A Next-Generation Sequencing Analysis
title_fullStr	Periodontal Pathogens Inhabit Root Caries Lesions Extending beyond the Gingival Margin: A Next-Generation Sequencing Analysis
title_full_unstemmed	Periodontal Pathogens Inhabit Root Caries Lesions Extending beyond the Gingival Margin: A Next-Generation Sequencing Analysis
title_sort	periodontal pathogens inhabit root caries lesions extending beyond the gingival margin: a next-generation sequencing analysis
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/68f422e2e8b34d33b4f8e7f761330308
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_version_	1718411201509064704

Periodontal Pathogens Inhabit Root Caries Lesions Extending beyond the Gingival Margin: A Next-Generation Sequencing Analysis

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