In vitro and in vivo effects of some chemical fungicides against Pythium ultimum and Phytophthora citrophthora associated with peach seedlings decline

Peach decline, responsible for seedlings root and collar rot in nurseries, is an important disease that causes reduction in plant production. Several oomycetes species were associated with this disease. The aim of this study was to control this serious peach decline disease using several assays such...

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Autores principales:	Sabrine Mannai, Naima Boughalleb-M’Hamdi
Formato:	article
Lenguaje:	EN
Publicado:	Ain Shams University 2021
Materias:	chemical fungicides nurseries peach decline pythium ultimum p. citrophthora Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/68f84bb5852c4e36a9acf31c430da844
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spelling	oai:doaj.org-article:68f84bb5852c4e36a9acf31c430da8442021-11-30T19:08:12ZIn vitro and in vivo effects of some chemical fungicides against Pythium ultimum and Phytophthora citrophthora associated with peach seedlings decline10.21608/nrmj.2021.2071662537-02862537-0294https://doaj.org/article/68f84bb5852c4e36a9acf31c430da8442021-11-01T00:00:00Zhttps://nrmj.journals.ekb.eg/article_207166_6fd4fea387ce93987c53dbb3e261a3e3.pdfhttps://doaj.org/toc/2537-0286https://doaj.org/toc/2537-0294Peach decline, responsible for seedlings root and collar rot in nurseries, is an important disease that causes reduction in plant production. Several oomycetes species were associated with this disease. The aim of this study was to control this serious peach decline disease using several assays such as; in vitro poisoned food technique and in vivo greenhouse assay. About six chemical fungicides were evaluated for their in vitro and in vivo inhibitory potentials against Pythium ultimum and Phytophthora citrophthora associated with this disease, respectively. The in vitro poisoned food technique demonstrated highly significant difference in the efficacy of the fungicides used at the five tested doses (10, 25, 50, 100 µg\ l and application rate). Carbendazim inhibited Pythium ultimum and P. citrophthora by 75.30 % and 100 % at 250 µg\ l. For Mancozeb, the inhibition % achieved by the registered dose was 100 % for Pythium ultimum and 50 % for P. citrophthora at 2000 µg\l. The registered dose (2000 µg\ l) of Fosetyl-Al inhibited Pythium ultimum and P. citrophthora by 51 % and 100 %, respectively. The highest rates of inhibition induced by Hymexazol were recorded at 60 µg\ l (90.55 % for Pythium ultimum and 94.49 % for P. citrophthora). In case of Chinosol, inhibition percentages of 90.30 % and 90.96 % for P. citrophthora and Pythium ultimum; respectively, were achieved at the tested concentration of 50 µg\ l, and the same inhibition values were also recorded for both tested concentrations of 100 and 2000 µg\ l. The highest inhibition rates for Metalaxyl-M against Pythium ultimum were observed at the dose of 100 µg\ l (79.70 %), whereas the used dose of 120 µg\ l recorded 86.59 %. The in vivo greenhouse assays demonstrated the efficacy of the Fosetyl-Al and Metalaxyl-M in reducing the peach seedling root browning induced by Pythium ultimum by 62.55 %. However, all the tested chemicals neither improved the growth and health status of the peach seedlings, nor reduced root browning of the seedlings inoculated with P. citrophthora.Sabrine MannaiNaima Boughalleb-M’HamdiAin Shams Universityarticlechemical fungicidesnurseriespeach declinepythium ultimump. citrophthoraMicrobiologyQR1-502ENNovel Research in Microbiology Journal, Vol 5, Iss 6, Pp 1431-1446 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	chemical fungicides nurseries peach decline pythium ultimum p. citrophthora Microbiology QR1-502
spellingShingle	chemical fungicides nurseries peach decline pythium ultimum p. citrophthora Microbiology QR1-502 Sabrine Mannai Naima Boughalleb-M’Hamdi In vitro and in vivo effects of some chemical fungicides against Pythium ultimum and Phytophthora citrophthora associated with peach seedlings decline
description	Peach decline, responsible for seedlings root and collar rot in nurseries, is an important disease that causes reduction in plant production. Several oomycetes species were associated with this disease. The aim of this study was to control this serious peach decline disease using several assays such as; in vitro poisoned food technique and in vivo greenhouse assay. About six chemical fungicides were evaluated for their in vitro and in vivo inhibitory potentials against Pythium ultimum and Phytophthora citrophthora associated with this disease, respectively. The in vitro poisoned food technique demonstrated highly significant difference in the efficacy of the fungicides used at the five tested doses (10, 25, 50, 100 µg\ l and application rate). Carbendazim inhibited Pythium ultimum and P. citrophthora by 75.30 % and 100 % at 250 µg\ l. For Mancozeb, the inhibition % achieved by the registered dose was 100 % for Pythium ultimum and 50 % for P. citrophthora at 2000 µg\l. The registered dose (2000 µg\ l) of Fosetyl-Al inhibited Pythium ultimum and P. citrophthora by 51 % and 100 %, respectively. The highest rates of inhibition induced by Hymexazol were recorded at 60 µg\ l (90.55 % for Pythium ultimum and 94.49 % for P. citrophthora). In case of Chinosol, inhibition percentages of 90.30 % and 90.96 % for P. citrophthora and Pythium ultimum; respectively, were achieved at the tested concentration of 50 µg\ l, and the same inhibition values were also recorded for both tested concentrations of 100 and 2000 µg\ l. The highest inhibition rates for Metalaxyl-M against Pythium ultimum were observed at the dose of 100 µg\ l (79.70 %), whereas the used dose of 120 µg\ l recorded 86.59 %. The in vivo greenhouse assays demonstrated the efficacy of the Fosetyl-Al and Metalaxyl-M in reducing the peach seedling root browning induced by Pythium ultimum by 62.55 %. However, all the tested chemicals neither improved the growth and health status of the peach seedlings, nor reduced root browning of the seedlings inoculated with P. citrophthora.
format	article
author	Sabrine Mannai Naima Boughalleb-M’Hamdi
author_facet	Sabrine Mannai Naima Boughalleb-M’Hamdi
author_sort	Sabrine Mannai
title	In vitro and in vivo effects of some chemical fungicides against Pythium ultimum and Phytophthora citrophthora associated with peach seedlings decline
title_short	In vitro and in vivo effects of some chemical fungicides against Pythium ultimum and Phytophthora citrophthora associated with peach seedlings decline
title_full	In vitro and in vivo effects of some chemical fungicides against Pythium ultimum and Phytophthora citrophthora associated with peach seedlings decline
title_fullStr	In vitro and in vivo effects of some chemical fungicides against Pythium ultimum and Phytophthora citrophthora associated with peach seedlings decline
title_full_unstemmed	In vitro and in vivo effects of some chemical fungicides against Pythium ultimum and Phytophthora citrophthora associated with peach seedlings decline
title_sort	in vitro and in vivo effects of some chemical fungicides against pythium ultimum and phytophthora citrophthora associated with peach seedlings decline
publisher	Ain Shams University
publishDate	2021
url	https://doaj.org/article/68f84bb5852c4e36a9acf31c430da844
work_keys_str_mv	AT sabrinemannai invitroandinvivoeffectsofsomechemicalfungicidesagainstpythiumultimumandphytophthoracitrophthoraassociatedwithpeachseedlingsdecline AT naimaboughallebmhamdi invitroandinvivoeffectsofsomechemicalfungicidesagainstpythiumultimumandphytophthoracitrophthoraassociatedwithpeachseedlingsdecline
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In vitro and in vivo effects of some chemical fungicides against Pythium ultimum and Phytophthora citrophthora associated with peach seedlings decline

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