Bone Formation Ability and Cell Viability Enhancement of MC3T3-E1 Cells by Ferrostatin-1 a Ferroptosis Inhibitor of Cancer Cells

Recently, ferroptosis has gained scientists’ attention as an iron-related regulated necrosis. However, not many reports have investigated the effect of ferroptosis on bone. Therefore, with the present study, we assessed the effect of ferroptosis inhibition using ferrostatin-1 on the MC3T3-E1 pre-ost...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Alireza Valanezhad, Tetsurou Odatsu, Shigeaki Abe, Ikuya Watanabe
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	ferrostatin-1 ferroptosis erastin regulated necrosis bone formation Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/68f948f07cb64531b37b54a9069119e4
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:68f948f07cb64531b37b54a9069119e4
record_format	dspace
spelling	oai:doaj.org-article:68f948f07cb64531b37b54a9069119e42021-11-25T17:54:50ZBone Formation Ability and Cell Viability Enhancement of MC3T3-E1 Cells by Ferrostatin-1 a Ferroptosis Inhibitor of Cancer Cells10.3390/ijms2222122591422-00671661-6596https://doaj.org/article/68f948f07cb64531b37b54a9069119e42021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12259https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Recently, ferroptosis has gained scientists’ attention as an iron-related regulated necrosis. However, not many reports have investigated the effect of ferroptosis on bone. Therefore, with the present study, we assessed the effect of ferroptosis inhibition using ferrostatin-1 on the MC3T3-E1 pre-osteoblast cell. Cell images, cell viability, alkaline phosphatase activity test, alizarin red staining, and RUNX2 gene expression using real-time PCR were applied to investigate the effects of ferrostatin and erastin on MC3T3-E1 osteoblast cells. Erastin was used as a well-known ferroptosis inducer reagent. Erastin with different concentrations ranging from 0 to 50 µmol/L was used for inducing cell death. The 25 µmol/L erastin led to controllable partial cell death on osteoblast cells. Ferrostatin-1 with 0 to 40 µmol/L was used for cell doping and cell death inhibition effect. Ferrostatin-1 also displayed a recovery effect on the samples, which had already received the partially artificial cell death by erastin. Cell differentiation, alizarin red staining, and RUNX2 gene expression confirmed the promotion of the bone formation ability effect of ferrostatin-1 on osteoblast cells. The objective of this study was to assess ferrostatin-1’s effect on the MC3T3-E1 osteoblast cell line based on its ferroptosis inhibitory property.Alireza ValanezhadTetsurou OdatsuShigeaki AbeIkuya WatanabeMDPI AGarticleferrostatin-1ferroptosiserastinregulated necrosisbone formationBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12259, p 12259 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	ferrostatin-1 ferroptosis erastin regulated necrosis bone formation Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	ferrostatin-1 ferroptosis erastin regulated necrosis bone formation Biology (General) QH301-705.5 Chemistry QD1-999 Alireza Valanezhad Tetsurou Odatsu Shigeaki Abe Ikuya Watanabe Bone Formation Ability and Cell Viability Enhancement of MC3T3-E1 Cells by Ferrostatin-1 a Ferroptosis Inhibitor of Cancer Cells
description	Recently, ferroptosis has gained scientists’ attention as an iron-related regulated necrosis. However, not many reports have investigated the effect of ferroptosis on bone. Therefore, with the present study, we assessed the effect of ferroptosis inhibition using ferrostatin-1 on the MC3T3-E1 pre-osteoblast cell. Cell images, cell viability, alkaline phosphatase activity test, alizarin red staining, and RUNX2 gene expression using real-time PCR were applied to investigate the effects of ferrostatin and erastin on MC3T3-E1 osteoblast cells. Erastin was used as a well-known ferroptosis inducer reagent. Erastin with different concentrations ranging from 0 to 50 µmol/L was used for inducing cell death. The 25 µmol/L erastin led to controllable partial cell death on osteoblast cells. Ferrostatin-1 with 0 to 40 µmol/L was used for cell doping and cell death inhibition effect. Ferrostatin-1 also displayed a recovery effect on the samples, which had already received the partially artificial cell death by erastin. Cell differentiation, alizarin red staining, and RUNX2 gene expression confirmed the promotion of the bone formation ability effect of ferrostatin-1 on osteoblast cells. The objective of this study was to assess ferrostatin-1’s effect on the MC3T3-E1 osteoblast cell line based on its ferroptosis inhibitory property.
format	article
author	Alireza Valanezhad Tetsurou Odatsu Shigeaki Abe Ikuya Watanabe
author_facet	Alireza Valanezhad Tetsurou Odatsu Shigeaki Abe Ikuya Watanabe
author_sort	Alireza Valanezhad
title	Bone Formation Ability and Cell Viability Enhancement of MC3T3-E1 Cells by Ferrostatin-1 a Ferroptosis Inhibitor of Cancer Cells
title_short	Bone Formation Ability and Cell Viability Enhancement of MC3T3-E1 Cells by Ferrostatin-1 a Ferroptosis Inhibitor of Cancer Cells
title_full	Bone Formation Ability and Cell Viability Enhancement of MC3T3-E1 Cells by Ferrostatin-1 a Ferroptosis Inhibitor of Cancer Cells
title_fullStr	Bone Formation Ability and Cell Viability Enhancement of MC3T3-E1 Cells by Ferrostatin-1 a Ferroptosis Inhibitor of Cancer Cells
title_full_unstemmed	Bone Formation Ability and Cell Viability Enhancement of MC3T3-E1 Cells by Ferrostatin-1 a Ferroptosis Inhibitor of Cancer Cells
title_sort	bone formation ability and cell viability enhancement of mc3t3-e1 cells by ferrostatin-1 a ferroptosis inhibitor of cancer cells
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/68f948f07cb64531b37b54a9069119e4
work_keys_str_mv	AT alirezavalanezhad boneformationabilityandcellviabilityenhancementofmc3t3e1cellsbyferrostatin1aferroptosisinhibitorofcancercells AT tetsurouodatsu boneformationabilityandcellviabilityenhancementofmc3t3e1cellsbyferrostatin1aferroptosisinhibitorofcancercells AT shigeakiabe boneformationabilityandcellviabilityenhancementofmc3t3e1cellsbyferrostatin1aferroptosisinhibitorofcancercells AT ikuyawatanabe boneformationabilityandcellviabilityenhancementofmc3t3e1cellsbyferrostatin1aferroptosisinhibitorofcancercells
_version_	1718411865900449792

Bone Formation Ability and Cell Viability Enhancement of MC3T3-E1 Cells by Ferrostatin-1 a Ferroptosis Inhibitor of Cancer Cells

Ejemplares similares