Establishment of an Efficient Micropropagation System for <i>Humulus lupulus</i> L. cv. Cascade and Confirmation of Genetic Uniformity of the Regenerated Plants through DNA Markers

The demand for virus-free hop planting material has increased in the last few years due to its multipurpose uses. The present study aimed to establish an effective protocol for clonal propagation of cv. Cascade using only the cytokinins as PGRs in all stages of micropropagation: (i) in vitro culture...

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Autores principales:	Doina Clapa, Monica Hârța
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	hop BA multiple shoot rooting–acclimatization gDNA genetic stability Agriculture S
Acceso en línea:	https://doaj.org/article/68fc44393ae54841ae998716c697c3b9
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spelling	oai:doaj.org-article:68fc44393ae54841ae998716c697c3b92021-11-25T16:09:30ZEstablishment of an Efficient Micropropagation System for <i>Humulus lupulus</i> L. cv. Cascade and Confirmation of Genetic Uniformity of the Regenerated Plants through DNA Markers10.3390/agronomy111122682073-4395https://doaj.org/article/68fc44393ae54841ae998716c697c3b92021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4395/11/11/2268https://doaj.org/toc/2073-4395The demand for virus-free hop planting material has increased in the last few years due to its multipurpose uses. The present study aimed to establish an effective protocol for clonal propagation of cv. Cascade using only the cytokinins as PGRs in all stages of micropropagation: (i) in vitro culture initiation using single-node micro-cuttings inoculated on modified Murashige and Skoog (MSm) medium solidified with Plant agar and supplemented with 0.5 mg L<sup>−1</sup> 6-benziyladenine (BA) with 76% recorded viability of nodal explants; (ii) in vitro multiplication of multinodal shoots on MSm medium gelled with Plant agar and supplemented with different types and concentrations of cytokinins: 2 mg L<sup>−1</sup> kinetin (KIN), 0.7 mg L<sup>−1</sup> 1-(2-Chloro-4-pyridyl)-3-phenylurea) (1 CPPU), 2 mg L<sup>−1</sup> meta-topoline (mT) and 0.5 mg L<sup>−1</sup> BA, which was the best variant for shoot proliferation (9.48 ± 0.78 shoots/explant); (iii) rooting and acclimatization with the best results obtained by ex vitro rooting and acclimatization of plants in the same stage in perlite (96.00 ± 0.60% acclimatized rooted plants with 100% survival under greenhouse conditions). The true-to-type nature of in vitro raised plants with the mother plant was assessed by Random Amplified Polymorphic DNA (RAPD) and Start Codon Target Polymorphism (SCoT) molecular markers, and then their genetic uniformity were confirmed.Doina ClapaMonica HârțaMDPI AGarticlehopBAmultiple shootrooting–acclimatizationgDNAgenetic stabilityAgricultureSENAgronomy, Vol 11, Iss 2268, p 2268 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	hop BA multiple shoot rooting–acclimatization gDNA genetic stability Agriculture S
spellingShingle	hop BA multiple shoot rooting–acclimatization gDNA genetic stability Agriculture S Doina Clapa Monica Hârța Establishment of an Efficient Micropropagation System for <i>Humulus lupulus</i> L. cv. Cascade and Confirmation of Genetic Uniformity of the Regenerated Plants through DNA Markers
description	The demand for virus-free hop planting material has increased in the last few years due to its multipurpose uses. The present study aimed to establish an effective protocol for clonal propagation of cv. Cascade using only the cytokinins as PGRs in all stages of micropropagation: (i) in vitro culture initiation using single-node micro-cuttings inoculated on modified Murashige and Skoog (MSm) medium solidified with Plant agar and supplemented with 0.5 mg L<sup>−1</sup> 6-benziyladenine (BA) with 76% recorded viability of nodal explants; (ii) in vitro multiplication of multinodal shoots on MSm medium gelled with Plant agar and supplemented with different types and concentrations of cytokinins: 2 mg L<sup>−1</sup> kinetin (KIN), 0.7 mg L<sup>−1</sup> 1-(2-Chloro-4-pyridyl)-3-phenylurea) (1 CPPU), 2 mg L<sup>−1</sup> meta-topoline (mT) and 0.5 mg L<sup>−1</sup> BA, which was the best variant for shoot proliferation (9.48 ± 0.78 shoots/explant); (iii) rooting and acclimatization with the best results obtained by ex vitro rooting and acclimatization of plants in the same stage in perlite (96.00 ± 0.60% acclimatized rooted plants with 100% survival under greenhouse conditions). The true-to-type nature of in vitro raised plants with the mother plant was assessed by Random Amplified Polymorphic DNA (RAPD) and Start Codon Target Polymorphism (SCoT) molecular markers, and then their genetic uniformity were confirmed.
format	article
author	Doina Clapa Monica Hârța
author_facet	Doina Clapa Monica Hârța
author_sort	Doina Clapa
title	Establishment of an Efficient Micropropagation System for <i>Humulus lupulus</i> L. cv. Cascade and Confirmation of Genetic Uniformity of the Regenerated Plants through DNA Markers
title_short	Establishment of an Efficient Micropropagation System for <i>Humulus lupulus</i> L. cv. Cascade and Confirmation of Genetic Uniformity of the Regenerated Plants through DNA Markers
title_full	Establishment of an Efficient Micropropagation System for <i>Humulus lupulus</i> L. cv. Cascade and Confirmation of Genetic Uniformity of the Regenerated Plants through DNA Markers
title_fullStr	Establishment of an Efficient Micropropagation System for <i>Humulus lupulus</i> L. cv. Cascade and Confirmation of Genetic Uniformity of the Regenerated Plants through DNA Markers
title_full_unstemmed	Establishment of an Efficient Micropropagation System for <i>Humulus lupulus</i> L. cv. Cascade and Confirmation of Genetic Uniformity of the Regenerated Plants through DNA Markers
title_sort	establishment of an efficient micropropagation system for <i>humulus lupulus</i> l. cv. cascade and confirmation of genetic uniformity of the regenerated plants through dna markers
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/68fc44393ae54841ae998716c697c3b9
work_keys_str_mv	AT doinaclapa establishmentofanefficientmicropropagationsystemforihumuluslupulusilcvcascadeandconfirmationofgeneticuniformityoftheregeneratedplantsthroughdnamarkers AT monicaharta establishmentofanefficientmicropropagationsystemforihumuluslupulusilcvcascadeandconfirmationofgeneticuniformityoftheregeneratedplantsthroughdnamarkers
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Establishment of an Efficient Micropropagation System for <i>Humulus lupulus</i> L. cv. Cascade and Confirmation of Genetic Uniformity of the Regenerated Plants through DNA Markers

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