3D printed microfluidic lab-on-a-chip device for fiber-based dual beam optical manipulation

Abstract 3D printing of microfluidic lab-on-a-chip devices enables rapid prototyping of robust and complex structures. In this work, we designed and fabricated a 3D printed lab-on-a-chip device for fiber-based dual beam optical manipulation. The final 3D printed chip offers three key features, such...

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Autores principales:	Haoran Wang, Anton Enders, John-Alexander Preuss, Janina Bahnemann, Alexander Heisterkamp, Maria Leilani Torres-Mapa
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6901502ef33540f8bb5d80b36a053aa5
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spelling	oai:doaj.org-article:6901502ef33540f8bb5d80b36a053aa52021-12-02T15:33:01Z3D printed microfluidic lab-on-a-chip device for fiber-based dual beam optical manipulation10.1038/s41598-021-93205-92045-2322https://doaj.org/article/6901502ef33540f8bb5d80b36a053aa52021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93205-9https://doaj.org/toc/2045-2322Abstract 3D printing of microfluidic lab-on-a-chip devices enables rapid prototyping of robust and complex structures. In this work, we designed and fabricated a 3D printed lab-on-a-chip device for fiber-based dual beam optical manipulation. The final 3D printed chip offers three key features, such as (1) an optimized fiber channel design for precise alignment of optical fibers, (2) an optically clear window to visualize the trapping region, and (3) a sample channel which facilitates hydrodynamic focusing of samples. A square zig–zag structure incorporated in the sample channel increases the number of particles at the trapping site and focuses the cells and particles during experiments when operating the chip at low Reynolds number. To evaluate the performance of the device for optical manipulation, we implemented on-chip, fiber-based optical trapping of different-sized microscopic particles and performed trap stiffness measurements. In addition, optical stretching of MCF-7 cells was successfully accomplished for the purpose of studying the effects of a cytochalasin metabolite, pyrichalasin H, on cell elasticity. We observed distinct changes in the deformability of single cells treated with pyrichalasin H compared to untreated cells. These results demonstrate that 3D printed microfluidic lab-on-a-chip devices offer a cost-effective and customizable platform for applications in optical manipulation.Haoran WangAnton EndersJohn-Alexander PreussJanina BahnemannAlexander HeisterkampMaria Leilani Torres-MapaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Haoran Wang Anton Enders John-Alexander Preuss Janina Bahnemann Alexander Heisterkamp Maria Leilani Torres-Mapa 3D printed microfluidic lab-on-a-chip device for fiber-based dual beam optical manipulation
description	Abstract 3D printing of microfluidic lab-on-a-chip devices enables rapid prototyping of robust and complex structures. In this work, we designed and fabricated a 3D printed lab-on-a-chip device for fiber-based dual beam optical manipulation. The final 3D printed chip offers three key features, such as (1) an optimized fiber channel design for precise alignment of optical fibers, (2) an optically clear window to visualize the trapping region, and (3) a sample channel which facilitates hydrodynamic focusing of samples. A square zig–zag structure incorporated in the sample channel increases the number of particles at the trapping site and focuses the cells and particles during experiments when operating the chip at low Reynolds number. To evaluate the performance of the device for optical manipulation, we implemented on-chip, fiber-based optical trapping of different-sized microscopic particles and performed trap stiffness measurements. In addition, optical stretching of MCF-7 cells was successfully accomplished for the purpose of studying the effects of a cytochalasin metabolite, pyrichalasin H, on cell elasticity. We observed distinct changes in the deformability of single cells treated with pyrichalasin H compared to untreated cells. These results demonstrate that 3D printed microfluidic lab-on-a-chip devices offer a cost-effective and customizable platform for applications in optical manipulation.
format	article
author	Haoran Wang Anton Enders John-Alexander Preuss Janina Bahnemann Alexander Heisterkamp Maria Leilani Torres-Mapa
author_facet	Haoran Wang Anton Enders John-Alexander Preuss Janina Bahnemann Alexander Heisterkamp Maria Leilani Torres-Mapa
author_sort	Haoran Wang
title	3D printed microfluidic lab-on-a-chip device for fiber-based dual beam optical manipulation
title_short	3D printed microfluidic lab-on-a-chip device for fiber-based dual beam optical manipulation
title_full	3D printed microfluidic lab-on-a-chip device for fiber-based dual beam optical manipulation
title_fullStr	3D printed microfluidic lab-on-a-chip device for fiber-based dual beam optical manipulation
title_full_unstemmed	3D printed microfluidic lab-on-a-chip device for fiber-based dual beam optical manipulation
title_sort	3d printed microfluidic lab-on-a-chip device for fiber-based dual beam optical manipulation
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/6901502ef33540f8bb5d80b36a053aa5
work_keys_str_mv	AT haoranwang 3dprintedmicrofluidiclabonachipdeviceforfiberbaseddualbeamopticalmanipulation AT antonenders 3dprintedmicrofluidiclabonachipdeviceforfiberbaseddualbeamopticalmanipulation AT johnalexanderpreuss 3dprintedmicrofluidiclabonachipdeviceforfiberbaseddualbeamopticalmanipulation AT janinabahnemann 3dprintedmicrofluidiclabonachipdeviceforfiberbaseddualbeamopticalmanipulation AT alexanderheisterkamp 3dprintedmicrofluidiclabonachipdeviceforfiberbaseddualbeamopticalmanipulation AT marialeilanitorresmapa 3dprintedmicrofluidiclabonachipdeviceforfiberbaseddualbeamopticalmanipulation
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3D printed microfluidic lab-on-a-chip device for fiber-based dual beam optical manipulation

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