Chimeric design of pyrrolysyl-tRNA synthetase/tRNA pairs and canonical synthetase/tRNA pairs for genetic code expansion

Orthogonal aminoacyl-tRNA synthetase/tRNA pairs are crucial for the incorporation of unnatural amino acids in a site-specific manner. Here the authors use rational chimera design to create multiple efficient pairs that function in bacterial and mammalian systems for genetic code expansion.

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Autores principales:	Wenlong Ding, Hongxia Zhao, Yulin Chen, Bin Zhang, Yang Yang, Jia Zang, Jing Wu, Shixian Lin
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/6902882d572e4b6385026c9aeca8abc6
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spelling	oai:doaj.org-article:6902882d572e4b6385026c9aeca8abc62021-12-02T17:12:20ZChimeric design of pyrrolysyl-tRNA synthetase/tRNA pairs and canonical synthetase/tRNA pairs for genetic code expansion10.1038/s41467-020-16898-y2041-1723https://doaj.org/article/6902882d572e4b6385026c9aeca8abc62020-06-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-16898-yhttps://doaj.org/toc/2041-1723Orthogonal aminoacyl-tRNA synthetase/tRNA pairs are crucial for the incorporation of unnatural amino acids in a site-specific manner. Here the authors use rational chimera design to create multiple efficient pairs that function in bacterial and mammalian systems for genetic code expansion.Wenlong DingHongxia ZhaoYulin ChenBin ZhangYang YangJia ZangJing WuShixian LinNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-13 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Wenlong Ding Hongxia Zhao Yulin Chen Bin Zhang Yang Yang Jia Zang Jing Wu Shixian Lin Chimeric design of pyrrolysyl-tRNA synthetase/tRNA pairs and canonical synthetase/tRNA pairs for genetic code expansion
description	Orthogonal aminoacyl-tRNA synthetase/tRNA pairs are crucial for the incorporation of unnatural amino acids in a site-specific manner. Here the authors use rational chimera design to create multiple efficient pairs that function in bacterial and mammalian systems for genetic code expansion.
format	article
author	Wenlong Ding Hongxia Zhao Yulin Chen Bin Zhang Yang Yang Jia Zang Jing Wu Shixian Lin
author_facet	Wenlong Ding Hongxia Zhao Yulin Chen Bin Zhang Yang Yang Jia Zang Jing Wu Shixian Lin
author_sort	Wenlong Ding
title	Chimeric design of pyrrolysyl-tRNA synthetase/tRNA pairs and canonical synthetase/tRNA pairs for genetic code expansion
title_short	Chimeric design of pyrrolysyl-tRNA synthetase/tRNA pairs and canonical synthetase/tRNA pairs for genetic code expansion
title_full	Chimeric design of pyrrolysyl-tRNA synthetase/tRNA pairs and canonical synthetase/tRNA pairs for genetic code expansion
title_fullStr	Chimeric design of pyrrolysyl-tRNA synthetase/tRNA pairs and canonical synthetase/tRNA pairs for genetic code expansion
title_full_unstemmed	Chimeric design of pyrrolysyl-tRNA synthetase/tRNA pairs and canonical synthetase/tRNA pairs for genetic code expansion
title_sort	chimeric design of pyrrolysyl-trna synthetase/trna pairs and canonical synthetase/trna pairs for genetic code expansion
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/6902882d572e4b6385026c9aeca8abc6
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Chimeric design of pyrrolysyl-tRNA synthetase/tRNA pairs and canonical synthetase/tRNA pairs for genetic code expansion

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