Rapid nanomolding of nanotopography on flexible substrates to control muscle cell growth with enhanced maturation

Abstract In vivo, multiple biophysical cues provided by highly ordered connective tissues of the extracellular matrix regulate skeletal muscle cells to align in parallel with one another. However, in routine in vitro cell culture environments, these key factors are often missing, which leads to chan...

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Autores principales:	Cong Wu, Chriss S. M. Chin, Qingyun Huang, Ho-Yin Chan, Xinge Yu, Vellaisamy A. L. Roy, Wen J. Li
Formato:	article
Lenguaje:	EN
Publicado:	Nature Publishing Group 2021
Materias:	Technology T Engineering (General). Civil engineering (General) TA1-2040
Acceso en línea:	https://doaj.org/article/69130cdb993a4c5ba283aa54ee50b7b6
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spelling	oai:doaj.org-article:69130cdb993a4c5ba283aa54ee50b7b62021-11-07T12:11:58ZRapid nanomolding of nanotopography on flexible substrates to control muscle cell growth with enhanced maturation10.1038/s41378-021-00316-42055-7434https://doaj.org/article/69130cdb993a4c5ba283aa54ee50b7b62021-11-01T00:00:00Zhttps://doi.org/10.1038/s41378-021-00316-4https://doaj.org/toc/2055-7434Abstract In vivo, multiple biophysical cues provided by highly ordered connective tissues of the extracellular matrix regulate skeletal muscle cells to align in parallel with one another. However, in routine in vitro cell culture environments, these key factors are often missing, which leads to changes in cell behavior. Here, we present a simple strategy for using optical media discs with nanogrooves and other polymer-based substrates nanomolded from the discs to directly culture muscle cells to study their response to the effect of biophysical cues such as nanotopography and substrate stiffness. We extend the range of study of biophysical cues for myoblasts by showing that they can sense ripple sizes as small as a 100 nm width and a 20 nm depth for myotube alignment, which has not been reported previously. The results revealed that nanotopography and substrate stiffness regulated myoblast proliferation and morphology independently, with nanotopographical cues showing a higher effect. These biophysical cues also worked synergistically, and their individual effects on cells were additive; i.e., by comparing cells grown on different polymer-based substrates (with and without nanogrooves), the cell proliferation rate could be reduced by as much as ~29%, and the elongation rate could be increased as much as ~116%. Moreover, during myogenesis, muscle cells actively responded to nanotopography and consistently showed increases in fusion and maturation indices of ~28% and ~21%, respectively. Finally, under electrical stimulation, the contraction amplitude of well-aligned myotubes was found to be almost 3 times greater than that for the cells on a smooth surface, regardless of the substrate stiffness.Cong WuChriss S. M. ChinQingyun HuangHo-Yin ChanXinge YuVellaisamy A. L. RoyWen J. LiNature Publishing GrouparticleTechnologyTEngineering (General). Civil engineering (General)TA1-2040ENMicrosystems & Nanoengineering, Vol 7, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Technology T Engineering (General). Civil engineering (General) TA1-2040
spellingShingle	Technology T Engineering (General). Civil engineering (General) TA1-2040 Cong Wu Chriss S. M. Chin Qingyun Huang Ho-Yin Chan Xinge Yu Vellaisamy A. L. Roy Wen J. Li Rapid nanomolding of nanotopography on flexible substrates to control muscle cell growth with enhanced maturation
description	Abstract In vivo, multiple biophysical cues provided by highly ordered connective tissues of the extracellular matrix regulate skeletal muscle cells to align in parallel with one another. However, in routine in vitro cell culture environments, these key factors are often missing, which leads to changes in cell behavior. Here, we present a simple strategy for using optical media discs with nanogrooves and other polymer-based substrates nanomolded from the discs to directly culture muscle cells to study their response to the effect of biophysical cues such as nanotopography and substrate stiffness. We extend the range of study of biophysical cues for myoblasts by showing that they can sense ripple sizes as small as a 100 nm width and a 20 nm depth for myotube alignment, which has not been reported previously. The results revealed that nanotopography and substrate stiffness regulated myoblast proliferation and morphology independently, with nanotopographical cues showing a higher effect. These biophysical cues also worked synergistically, and their individual effects on cells were additive; i.e., by comparing cells grown on different polymer-based substrates (with and without nanogrooves), the cell proliferation rate could be reduced by as much as ~29%, and the elongation rate could be increased as much as ~116%. Moreover, during myogenesis, muscle cells actively responded to nanotopography and consistently showed increases in fusion and maturation indices of ~28% and ~21%, respectively. Finally, under electrical stimulation, the contraction amplitude of well-aligned myotubes was found to be almost 3 times greater than that for the cells on a smooth surface, regardless of the substrate stiffness.
format	article
author	Cong Wu Chriss S. M. Chin Qingyun Huang Ho-Yin Chan Xinge Yu Vellaisamy A. L. Roy Wen J. Li
author_facet	Cong Wu Chriss S. M. Chin Qingyun Huang Ho-Yin Chan Xinge Yu Vellaisamy A. L. Roy Wen J. Li
author_sort	Cong Wu
title	Rapid nanomolding of nanotopography on flexible substrates to control muscle cell growth with enhanced maturation
title_short	Rapid nanomolding of nanotopography on flexible substrates to control muscle cell growth with enhanced maturation
title_full	Rapid nanomolding of nanotopography on flexible substrates to control muscle cell growth with enhanced maturation
title_fullStr	Rapid nanomolding of nanotopography on flexible substrates to control muscle cell growth with enhanced maturation
title_full_unstemmed	Rapid nanomolding of nanotopography on flexible substrates to control muscle cell growth with enhanced maturation
title_sort	rapid nanomolding of nanotopography on flexible substrates to control muscle cell growth with enhanced maturation
publisher	Nature Publishing Group
publishDate	2021
url	https://doaj.org/article/69130cdb993a4c5ba283aa54ee50b7b6
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Rapid nanomolding of nanotopography on flexible substrates to control muscle cell growth with enhanced maturation

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