Novel bacteriophages containing a genome of another bacteriophage within their genomes.

A novel bacteriophage infecting Staphylococus pasteuri was isolated during a screen for phages in Antarctic soils. The phage named SpaA1 is morphologically similar to phages of the family Siphoviridae. The 42,784 bp genome of SpaA1 is a linear, double-stranded DNA molecule with 3' protruding co...

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Autores principales:	Maud M Swanson, Brian Reavy, Kira S Makarova, Peter J Cock, David W Hopkins, Lesley Torrance, Eugene V Koonin, Michael Taliansky
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/691b712c3b43494a92478022c1271e47
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spelling	oai:doaj.org-article:691b712c3b43494a92478022c1271e472021-11-18T07:12:15ZNovel bacteriophages containing a genome of another bacteriophage within their genomes.1932-620310.1371/journal.pone.0040683https://doaj.org/article/691b712c3b43494a92478022c1271e472012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22815791/?tool=EBIhttps://doaj.org/toc/1932-6203A novel bacteriophage infecting Staphylococus pasteuri was isolated during a screen for phages in Antarctic soils. The phage named SpaA1 is morphologically similar to phages of the family Siphoviridae. The 42,784 bp genome of SpaA1 is a linear, double-stranded DNA molecule with 3' protruding cohesive ends. The SpaA1 genome encompasses 63 predicted protein-coding genes which cluster within three regions of the genome, each of apparently different origin, in a mosaic pattern. In two of these regions, the gene sets resemble those in prophages of Bacillus thuringiensis kurstaki str. T03a001 (genes involved in DNA replication/transcription, cell entry and exit) and B. cereus AH676 (additional regulatory and recombination genes), respectively. The third region represents an almost complete genome (except for the short terminal segments) of a distinct bacteriophage, MZTP02. Nearly the same gene module was identified in prophages of B. thuringiensis serovar monterrey BGSC 4AJ1 and B. cereus Rock4-2. These findings suggest that MZTP02 can be shuttled between genomes of other bacteriophages and prophages, leading to the formation of chimeric genomes. The presence of a complete phage genome in the genome of other phages apparently has not been described previously and might represent a 'fast track' route of virus evolution and horizontal gene transfer. Another phage (BceA1) nearly identical in sequence to SpaA1, and also including the almost complete MZTP02 genome within its own genome, was isolated from a bacterium of the B. cereus/B. thuringiensis group. Remarkably, both SpaA1 and BceA1 phages can infect B. cereus and B. thuringiensis, but only one of them, SpaA1, can infect S. pasteuri. This finding is best compatible with a scenario in which MZTP02 was originally contained in BceA1 infecting Bacillus spp, the common hosts for these two phages, followed by emergence of SpaA1 infecting S. pasteuri.Maud M SwansonBrian ReavyKira S MakarovaPeter J CockDavid W HopkinsLesley TorranceEugene V KooninMichael TalianskyPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 7, p e40683 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Maud M Swanson Brian Reavy Kira S Makarova Peter J Cock David W Hopkins Lesley Torrance Eugene V Koonin Michael Taliansky Novel bacteriophages containing a genome of another bacteriophage within their genomes.
description	A novel bacteriophage infecting Staphylococus pasteuri was isolated during a screen for phages in Antarctic soils. The phage named SpaA1 is morphologically similar to phages of the family Siphoviridae. The 42,784 bp genome of SpaA1 is a linear, double-stranded DNA molecule with 3' protruding cohesive ends. The SpaA1 genome encompasses 63 predicted protein-coding genes which cluster within three regions of the genome, each of apparently different origin, in a mosaic pattern. In two of these regions, the gene sets resemble those in prophages of Bacillus thuringiensis kurstaki str. T03a001 (genes involved in DNA replication/transcription, cell entry and exit) and B. cereus AH676 (additional regulatory and recombination genes), respectively. The third region represents an almost complete genome (except for the short terminal segments) of a distinct bacteriophage, MZTP02. Nearly the same gene module was identified in prophages of B. thuringiensis serovar monterrey BGSC 4AJ1 and B. cereus Rock4-2. These findings suggest that MZTP02 can be shuttled between genomes of other bacteriophages and prophages, leading to the formation of chimeric genomes. The presence of a complete phage genome in the genome of other phages apparently has not been described previously and might represent a 'fast track' route of virus evolution and horizontal gene transfer. Another phage (BceA1) nearly identical in sequence to SpaA1, and also including the almost complete MZTP02 genome within its own genome, was isolated from a bacterium of the B. cereus/B. thuringiensis group. Remarkably, both SpaA1 and BceA1 phages can infect B. cereus and B. thuringiensis, but only one of them, SpaA1, can infect S. pasteuri. This finding is best compatible with a scenario in which MZTP02 was originally contained in BceA1 infecting Bacillus spp, the common hosts for these two phages, followed by emergence of SpaA1 infecting S. pasteuri.
format	article
author	Maud M Swanson Brian Reavy Kira S Makarova Peter J Cock David W Hopkins Lesley Torrance Eugene V Koonin Michael Taliansky
author_facet	Maud M Swanson Brian Reavy Kira S Makarova Peter J Cock David W Hopkins Lesley Torrance Eugene V Koonin Michael Taliansky
author_sort	Maud M Swanson
title	Novel bacteriophages containing a genome of another bacteriophage within their genomes.
title_short	Novel bacteriophages containing a genome of another bacteriophage within their genomes.
title_full	Novel bacteriophages containing a genome of another bacteriophage within their genomes.
title_fullStr	Novel bacteriophages containing a genome of another bacteriophage within their genomes.
title_full_unstemmed	Novel bacteriophages containing a genome of another bacteriophage within their genomes.
title_sort	novel bacteriophages containing a genome of another bacteriophage within their genomes.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/691b712c3b43494a92478022c1271e47
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Novel bacteriophages containing a genome of another bacteriophage within their genomes.

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