Nicotinamide mononucleotide production by fructophilic lactic acid bacteria

Abstract Nicotinamide mononucleotide (NMN), an intermediate in nicotinamide adenine dinucleotide biosynthesis, is recently attracting much attention for its pharmacological and anti-aging efficacies. However, current commercial products containing NMN are very high-priced because efficient and facil...

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Autores principales:	Kazane Sugiyama, Kana Iijima, Miyako Yoshino, Hideo Dohra, Yuji Tokimoto, Koji Nishikawa, Hideaki Idogaki, Nobuyuki Yoshida
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/69217ad54c3d43968ea789c729692bf6
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spelling	oai:doaj.org-article:69217ad54c3d43968ea789c729692bf62021-12-02T14:15:53ZNicotinamide mononucleotide production by fructophilic lactic acid bacteria10.1038/s41598-021-87361-12045-2322https://doaj.org/article/69217ad54c3d43968ea789c729692bf62021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87361-1https://doaj.org/toc/2045-2322Abstract Nicotinamide mononucleotide (NMN), an intermediate in nicotinamide adenine dinucleotide biosynthesis, is recently attracting much attention for its pharmacological and anti-aging efficacies. However, current commercial products containing NMN are very high-priced because efficient and facile methods for industrial NMN production are limited. In this study, aiming for its nutraceutical application, we attempted to screen lactic acid bacteria for intracellular and/or extracellular NMN production. Using a bioassay system with an auxotrophic yeast that requires nicotinamide riboside (NR; dephosphorylated NMN), three candidates were obtained from a library of 174 strains of facultative anaerobic lactic acid bacteria. All three candidates belonged to the genus Fructobacillus and produced NR in the culture media (0.8–1.5 mg/l). Lactic acid bacteria of the genus Fructobacillus are known to use d-fructose as an electron acceptor in anaerobic lactic acid fermentation; addition of d-fructose to the medium caused intracellular accumulation of NMN and NR, but no extracellular production of these compounds was observed. Draft genome sequencing for one of the candidates suggested that nicotinamide phosphoribosyltransferase, which exists commonly in mammals but is less reported in microorganisms, is a key enzyme for NMN and NR production in the fructophilic bacteria.Kazane SugiyamaKana IijimaMiyako YoshinoHideo DohraYuji TokimotoKoji NishikawaHideaki IdogakiNobuyuki YoshidaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kazane Sugiyama Kana Iijima Miyako Yoshino Hideo Dohra Yuji Tokimoto Koji Nishikawa Hideaki Idogaki Nobuyuki Yoshida Nicotinamide mononucleotide production by fructophilic lactic acid bacteria
description	Abstract Nicotinamide mononucleotide (NMN), an intermediate in nicotinamide adenine dinucleotide biosynthesis, is recently attracting much attention for its pharmacological and anti-aging efficacies. However, current commercial products containing NMN are very high-priced because efficient and facile methods for industrial NMN production are limited. In this study, aiming for its nutraceutical application, we attempted to screen lactic acid bacteria for intracellular and/or extracellular NMN production. Using a bioassay system with an auxotrophic yeast that requires nicotinamide riboside (NR; dephosphorylated NMN), three candidates were obtained from a library of 174 strains of facultative anaerobic lactic acid bacteria. All three candidates belonged to the genus Fructobacillus and produced NR in the culture media (0.8–1.5 mg/l). Lactic acid bacteria of the genus Fructobacillus are known to use d-fructose as an electron acceptor in anaerobic lactic acid fermentation; addition of d-fructose to the medium caused intracellular accumulation of NMN and NR, but no extracellular production of these compounds was observed. Draft genome sequencing for one of the candidates suggested that nicotinamide phosphoribosyltransferase, which exists commonly in mammals but is less reported in microorganisms, is a key enzyme for NMN and NR production in the fructophilic bacteria.
format	article
author	Kazane Sugiyama Kana Iijima Miyako Yoshino Hideo Dohra Yuji Tokimoto Koji Nishikawa Hideaki Idogaki Nobuyuki Yoshida
author_facet	Kazane Sugiyama Kana Iijima Miyako Yoshino Hideo Dohra Yuji Tokimoto Koji Nishikawa Hideaki Idogaki Nobuyuki Yoshida
author_sort	Kazane Sugiyama
title	Nicotinamide mononucleotide production by fructophilic lactic acid bacteria
title_short	Nicotinamide mononucleotide production by fructophilic lactic acid bacteria
title_full	Nicotinamide mononucleotide production by fructophilic lactic acid bacteria
title_fullStr	Nicotinamide mononucleotide production by fructophilic lactic acid bacteria
title_full_unstemmed	Nicotinamide mononucleotide production by fructophilic lactic acid bacteria
title_sort	nicotinamide mononucleotide production by fructophilic lactic acid bacteria
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/69217ad54c3d43968ea789c729692bf6
work_keys_str_mv	AT kazanesugiyama nicotinamidemononucleotideproductionbyfructophiliclacticacidbacteria AT kanaiijima nicotinamidemononucleotideproductionbyfructophiliclacticacidbacteria AT miyakoyoshino nicotinamidemononucleotideproductionbyfructophiliclacticacidbacteria AT hideodohra nicotinamidemononucleotideproductionbyfructophiliclacticacidbacteria AT yujitokimoto nicotinamidemononucleotideproductionbyfructophiliclacticacidbacteria AT kojinishikawa nicotinamidemononucleotideproductionbyfructophiliclacticacidbacteria AT hideakiidogaki nicotinamidemononucleotideproductionbyfructophiliclacticacidbacteria AT nobuyukiyoshida nicotinamidemononucleotideproductionbyfructophiliclacticacidbacteria
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Nicotinamide mononucleotide production by fructophilic lactic acid bacteria

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