Roles of GalNAc-disialyl Lactotetraosyl Antigens in Renal Cancer Cells

Abstract GalNAc-disialyl Lc4 (GalNAc-DSLc4) was reported as a novel antigen that associated with malignant features of renal cell cancers (RCCs). To clarify roles of GalNAc-DSLc4 in malignant properties of RCCs, we identified B4GalNAc-T2 as a responsible gene for the synthesis of GalNAc-DSLc4, and p...

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Autores principales:	Akiko Tsuchida, Motohiro Senda, Akihiro Ito, Seiichi Saito, Makoto Kiso, Takayuki Ando, Anne Harduin-Lepers, Akio Matsuda, Keiko Furukawa, Koichi Furukawa
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6931623c8cee4e688a3f16eaa3d0c741
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spelling	oai:doaj.org-article:6931623c8cee4e688a3f16eaa3d0c7412021-12-02T15:08:14ZRoles of GalNAc-disialyl Lactotetraosyl Antigens in Renal Cancer Cells10.1038/s41598-018-25521-62045-2322https://doaj.org/article/6931623c8cee4e688a3f16eaa3d0c7412018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25521-6https://doaj.org/toc/2045-2322Abstract GalNAc-disialyl Lc4 (GalNAc-DSLc4) was reported as a novel antigen that associated with malignant features of renal cell cancers (RCCs). To clarify roles of GalNAc-DSLc4 in malignant properties of RCCs, we identified B4GalNAc-T2 as a responsible gene for the synthesis of GalNAc-DSLc4, and prepared stable transfectants of GalNAc-T2 cDNA using VMRC-RCW cells, resulting in the establishment of high expressants of GalNAc-DSLc4. They showed increased proliferation and invasion, and specific adhesion to laminin. In the transfectants, PI3K/Akt signals were highly activated by serum stimulation or adhesion to laminin. GalNAc-DSLc4 was co-localized in lipid rafts with integrin β1 and caveolin-1 in both immunoblotting of fractionated detergent extracts and immunocytostaining, particularly when stimulated with serum. Masking of GalNAc-DSLc4 with antibodies as well as PI3K inhibitor suppressed malignant properties of the transfectants. These results suggested that GalNAc-DSLc4 is involved in malignant properties of RCCs by forming a molecular complex with integrins in lipid rafts.Akiko TsuchidaMotohiro SendaAkihiro ItoSeiichi SaitoMakoto KisoTakayuki AndoAnne Harduin-LepersAkio MatsudaKeiko FurukawaKoichi FurukawaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-15 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Akiko Tsuchida Motohiro Senda Akihiro Ito Seiichi Saito Makoto Kiso Takayuki Ando Anne Harduin-Lepers Akio Matsuda Keiko Furukawa Koichi Furukawa Roles of GalNAc-disialyl Lactotetraosyl Antigens in Renal Cancer Cells
description	Abstract GalNAc-disialyl Lc4 (GalNAc-DSLc4) was reported as a novel antigen that associated with malignant features of renal cell cancers (RCCs). To clarify roles of GalNAc-DSLc4 in malignant properties of RCCs, we identified B4GalNAc-T2 as a responsible gene for the synthesis of GalNAc-DSLc4, and prepared stable transfectants of GalNAc-T2 cDNA using VMRC-RCW cells, resulting in the establishment of high expressants of GalNAc-DSLc4. They showed increased proliferation and invasion, and specific adhesion to laminin. In the transfectants, PI3K/Akt signals were highly activated by serum stimulation or adhesion to laminin. GalNAc-DSLc4 was co-localized in lipid rafts with integrin β1 and caveolin-1 in both immunoblotting of fractionated detergent extracts and immunocytostaining, particularly when stimulated with serum. Masking of GalNAc-DSLc4 with antibodies as well as PI3K inhibitor suppressed malignant properties of the transfectants. These results suggested that GalNAc-DSLc4 is involved in malignant properties of RCCs by forming a molecular complex with integrins in lipid rafts.
format	article
author	Akiko Tsuchida Motohiro Senda Akihiro Ito Seiichi Saito Makoto Kiso Takayuki Ando Anne Harduin-Lepers Akio Matsuda Keiko Furukawa Koichi Furukawa
author_facet	Akiko Tsuchida Motohiro Senda Akihiro Ito Seiichi Saito Makoto Kiso Takayuki Ando Anne Harduin-Lepers Akio Matsuda Keiko Furukawa Koichi Furukawa
author_sort	Akiko Tsuchida
title	Roles of GalNAc-disialyl Lactotetraosyl Antigens in Renal Cancer Cells
title_short	Roles of GalNAc-disialyl Lactotetraosyl Antigens in Renal Cancer Cells
title_full	Roles of GalNAc-disialyl Lactotetraosyl Antigens in Renal Cancer Cells
title_fullStr	Roles of GalNAc-disialyl Lactotetraosyl Antigens in Renal Cancer Cells
title_full_unstemmed	Roles of GalNAc-disialyl Lactotetraosyl Antigens in Renal Cancer Cells
title_sort	roles of galnac-disialyl lactotetraosyl antigens in renal cancer cells
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/6931623c8cee4e688a3f16eaa3d0c741
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Roles of GalNAc-disialyl Lactotetraosyl Antigens in Renal Cancer Cells

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