Tracking the formation and degradation of fatty-acid-accumulated mitochondria using label-free chemical imaging

Abstract The mitochondrion is one of the key organelles for maintaining cellular homeostasis. External environmental stimuli and internal regulatory processes may alter the metabolism and functions of mitochondria. To understand these activities of mitochondria, it is critical to probe the key metab...

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Autores principales:	Chi Zhang, Stephen A. Boppart
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/69345381141941ef86a0349a2c341a93
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spelling	oai:doaj.org-article:69345381141941ef86a0349a2c341a932021-12-02T16:36:13ZTracking the formation and degradation of fatty-acid-accumulated mitochondria using label-free chemical imaging10.1038/s41598-021-85795-12045-2322https://doaj.org/article/69345381141941ef86a0349a2c341a932021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85795-1https://doaj.org/toc/2045-2322Abstract The mitochondrion is one of the key organelles for maintaining cellular homeostasis. External environmental stimuli and internal regulatory processes may alter the metabolism and functions of mitochondria. To understand these activities of mitochondria, it is critical to probe the key metabolic molecules inside these organelles. In this study, we used label-free chemical imaging modalities including coherent anti-Stokes Raman scattering and multiphoton-excited fluorescence to investigate the mitochondrial activities in living cancer cells. We found that hypothermia exposure tends to induce fatty-acid (FA) accumulation in some mitochondria of MIAPaCa-2 cells. Autofluorescence images show that the FA-accumulated mitochondria also have abnormal metabolism of nicotinamide adenine dinucleotide hydrogen, likely induced by the dysfunction of the electron transport chain. We also found that when the cells were re-warmed to physiological temperature after a period of hypothermia, the FA-accumulated mitochondria changed their structural features. To the best of our knowledge, this is the first time that FA accumulation in mitochondria was observed in live cells. Our research also demonstrates that multimodal label-free chemical imaging is an attractive tool to discover abnormal functions of mitochondria at the single-organelle level and can be used to quantify the dynamic changes of these organelles under perturbative conditions.Chi ZhangStephen A. BoppartNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chi Zhang Stephen A. Boppart Tracking the formation and degradation of fatty-acid-accumulated mitochondria using label-free chemical imaging
description	Abstract The mitochondrion is one of the key organelles for maintaining cellular homeostasis. External environmental stimuli and internal regulatory processes may alter the metabolism and functions of mitochondria. To understand these activities of mitochondria, it is critical to probe the key metabolic molecules inside these organelles. In this study, we used label-free chemical imaging modalities including coherent anti-Stokes Raman scattering and multiphoton-excited fluorescence to investigate the mitochondrial activities in living cancer cells. We found that hypothermia exposure tends to induce fatty-acid (FA) accumulation in some mitochondria of MIAPaCa-2 cells. Autofluorescence images show that the FA-accumulated mitochondria also have abnormal metabolism of nicotinamide adenine dinucleotide hydrogen, likely induced by the dysfunction of the electron transport chain. We also found that when the cells were re-warmed to physiological temperature after a period of hypothermia, the FA-accumulated mitochondria changed their structural features. To the best of our knowledge, this is the first time that FA accumulation in mitochondria was observed in live cells. Our research also demonstrates that multimodal label-free chemical imaging is an attractive tool to discover abnormal functions of mitochondria at the single-organelle level and can be used to quantify the dynamic changes of these organelles under perturbative conditions.
format	article
author	Chi Zhang Stephen A. Boppart
author_facet	Chi Zhang Stephen A. Boppart
author_sort	Chi Zhang
title	Tracking the formation and degradation of fatty-acid-accumulated mitochondria using label-free chemical imaging
title_short	Tracking the formation and degradation of fatty-acid-accumulated mitochondria using label-free chemical imaging
title_full	Tracking the formation and degradation of fatty-acid-accumulated mitochondria using label-free chemical imaging
title_fullStr	Tracking the formation and degradation of fatty-acid-accumulated mitochondria using label-free chemical imaging
title_full_unstemmed	Tracking the formation and degradation of fatty-acid-accumulated mitochondria using label-free chemical imaging
title_sort	tracking the formation and degradation of fatty-acid-accumulated mitochondria using label-free chemical imaging
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/69345381141941ef86a0349a2c341a93
work_keys_str_mv	AT chizhang trackingtheformationanddegradationoffattyacidaccumulatedmitochondriausinglabelfreechemicalimaging AT stephenaboppart trackingtheformationanddegradationoffattyacidaccumulatedmitochondriausinglabelfreechemicalimaging
_version_	1718383637936734208

Tracking the formation and degradation of fatty-acid-accumulated mitochondria using label-free chemical imaging

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