Prevalence and molecular-genetic characteristics of hepatitis B virus in HIV-positive individuals at the Far Eastern Federal District

Prevalence and molecular-genetic characteristics of hepatitis B virus in HIV-positive individuals at the Far Eastern Federal District

Current study was aimed at investigating prevalence of overt and occult hepatitis B infection in HIV-positive individuals as well as molecular-epidemiological characteristics of the circulating hepatitis B virus (HBV) strains in the Far Eastern Federal District (FEFD). A total number of 297 blood se...

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Autores principales: E. A. Bazykina, V. B. Turkutyukov, O. E. Trotsenko, V. O. Kotova, L. A. Balakhontseva, L. V. Butakova, L. V. Varnavskaya, V. V. Fomenko, M. A. Chechulina, A. I. Krapivkin, S. V. Zinkovskaya, G. I. Budai, O. A. Yanovich, E. A. Lomakina, Z. I. Lukina
Formato: article
Lenguaje:RU
Publicado: Sankt-Peterburg : NIIÈM imeni Pastera 2019
Materias:
hiv-infection
viral hepatitis b
molecular epidemiology
coinfection
far eastern federal district
prevalence
Infectious and parasitic diseases
RC109-216
Acceso en línea:https://doaj.org/article/693ff6f20eef4e5288b49d4e3b461784
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spelling oai:doaj.org-article:693ff6f20eef4e5288b49d4e3b4617842021-11-22T07:09:52ZPrevalence and molecular-genetic characteristics of hepatitis B virus in HIV-positive individuals at the Far Eastern Federal District2220-76192313-739810.15789/2220-7619-2019-1-183-192https://doaj.org/article/693ff6f20eef4e5288b49d4e3b4617842019-05-01T00:00:00Zhttps://www.iimmun.ru/iimm/article/view/748https://doaj.org/toc/2220-7619https://doaj.org/toc/2313-7398Current study was aimed at investigating prevalence of overt and occult hepatitis B infection in HIV-positive individuals as well as molecular-epidemiological characteristics of the circulating hepatitis B virus (HBV) strains in the Far Eastern Federal District (FEFD). A total number of 297 blood serum/plasma samples obtained from HIV-positive patients residing in the FEFD were enrolled in the study. The first control group included 351 blood serum/plasma samples of general population without indication on HIV and HBV-infection that underwent laboratory check up at the Centers for AIDS Prevention and Control. After evaluating the group of HIV-positive patients 20 HIV-HBV positive samples were selected for further detailed analysis. The second control group included 43 patients with chronic hepatitis B. All groups were age and gender-matched. The research included serological and molecular-genetic (real-time PCR, positive for HBV DNA samples underwent clonal sequencing of PCR-amplified HBV P/S gene) assessment of the biological material followed by a phylogenetic analysis of the HBV sequences. Our research revealed that HIV-positive patients are exposed to a higher risk of HBV infection compared to general population enrolled in the study, which is evident from the prevalence of anti-HBcAg antibodies in the groups examined. HIV-positive vs. first control group was positive for anti-HBcAg antibodies at higher rate (35.02%, CI 95: 29.59–40.45% versus 22.22%, CI 95: 17.87–26.57%, p = 0.0003). Abundance of ongoing HBV-infection markers was also higher in HIV-positive individuals compared to general population (6.73%, CI 95: 3.88–9.58% versus 0.85%, CI 95: 0–1.81%, p = 0.0001, respectively). Should be noted, that HBsAg-negative HBV infection in HIV-positive patient cohort comprised 1.01% (CI 95: 0–2.15%) whereas in general population this index was as low as 0.28% (CI 95: 0–0.84%). Virus hepatitis С and D were revealed at higher rate in HIV-positive individuals compared to patients with HIV-negative chronic HBV infection (p = 5.84 × 10–7  and p = 0.000001 respectively). HCV and HDV prevalence rates comprised 50.0% (CI 95: 27.46–77.46%) and 40.0% (CI 95: 17.97–62.03%) in HIV-positive patients. Similar indices in control group were 4.65% (CI 95: 0–10.94%) and 4.65% (CI 95: 0–10.94%), respectively. The phylogenetic analysis of the six isolated HBV sequences showed that the five samples belonged to genotype D, with dominant subtype D2 (verified in 4 cases). Further, HBV genotype С was detected only in one case. The obtained data indicate a necessity for further in-depth diagnostic examination of viral hepatitis in HIV-positive patients to lower a risk of developing life-threatening complications as well as preventing hepatitis spread in human population.E. A. BazykinaV. B. TurkutyukovO. E. TrotsenkoV. O. KotovaL. A. BalakhontsevaL. V. ButakovaL. V. VarnavskayaV. V. FomenkoM. A. ChechulinaA. I. KrapivkinS. V. ZinkovskayaG. I. BudaiO. A. YanovichE. A. LomakinaZ. I. LukinaSankt-Peterburg : NIIÈM imeni Pasteraarticlehiv-infectionviral hepatitis bmolecular epidemiologycoinfectionfar eastern federal districtprevalenceInfectious and parasitic diseasesRC109-216RUInfekciâ i Immunitet, Vol 9, Iss 1, Pp 183-192 (2019)
institution DOAJ
collection DOAJ
language RU
topic hiv-infection
viral hepatitis b
molecular epidemiology
coinfection
far eastern federal district
prevalence
Infectious and parasitic diseases
RC109-216
spellingShingle hiv-infection
viral hepatitis b
molecular epidemiology
coinfection
far eastern federal district
prevalence
Infectious and parasitic diseases
RC109-216
E. A. Bazykina
V. B. Turkutyukov
O. E. Trotsenko
V. O. Kotova
L. A. Balakhontseva
L. V. Butakova
L. V. Varnavskaya
V. V. Fomenko
M. A. Chechulina
A. I. Krapivkin
S. V. Zinkovskaya
G. I. Budai
O. A. Yanovich
E. A. Lomakina
Z. I. Lukina
Prevalence and molecular-genetic characteristics of hepatitis B virus in HIV-positive individuals at the Far Eastern Federal District
description Current study was aimed at investigating prevalence of overt and occult hepatitis B infection in HIV-positive individuals as well as molecular-epidemiological characteristics of the circulating hepatitis B virus (HBV) strains in the Far Eastern Federal District (FEFD). A total number of 297 blood serum/plasma samples obtained from HIV-positive patients residing in the FEFD were enrolled in the study. The first control group included 351 blood serum/plasma samples of general population without indication on HIV and HBV-infection that underwent laboratory check up at the Centers for AIDS Prevention and Control. After evaluating the group of HIV-positive patients 20 HIV-HBV positive samples were selected for further detailed analysis. The second control group included 43 patients with chronic hepatitis B. All groups were age and gender-matched. The research included serological and molecular-genetic (real-time PCR, positive for HBV DNA samples underwent clonal sequencing of PCR-amplified HBV P/S gene) assessment of the biological material followed by a phylogenetic analysis of the HBV sequences. Our research revealed that HIV-positive patients are exposed to a higher risk of HBV infection compared to general population enrolled in the study, which is evident from the prevalence of anti-HBcAg antibodies in the groups examined. HIV-positive vs. first control group was positive for anti-HBcAg antibodies at higher rate (35.02%, CI 95: 29.59–40.45% versus 22.22%, CI 95: 17.87–26.57%, p = 0.0003). Abundance of ongoing HBV-infection markers was also higher in HIV-positive individuals compared to general population (6.73%, CI 95: 3.88–9.58% versus 0.85%, CI 95: 0–1.81%, p = 0.0001, respectively). Should be noted, that HBsAg-negative HBV infection in HIV-positive patient cohort comprised 1.01% (CI 95: 0–2.15%) whereas in general population this index was as low as 0.28% (CI 95: 0–0.84%). Virus hepatitis С and D were revealed at higher rate in HIV-positive individuals compared to patients with HIV-negative chronic HBV infection (p = 5.84 × 10–7  and p = 0.000001 respectively). HCV and HDV prevalence rates comprised 50.0% (CI 95: 27.46–77.46%) and 40.0% (CI 95: 17.97–62.03%) in HIV-positive patients. Similar indices in control group were 4.65% (CI 95: 0–10.94%) and 4.65% (CI 95: 0–10.94%), respectively. The phylogenetic analysis of the six isolated HBV sequences showed that the five samples belonged to genotype D, with dominant subtype D2 (verified in 4 cases). Further, HBV genotype С was detected only in one case. The obtained data indicate a necessity for further in-depth diagnostic examination of viral hepatitis in HIV-positive patients to lower a risk of developing life-threatening complications as well as preventing hepatitis spread in human population.
format article
author E. A. Bazykina
V. B. Turkutyukov
O. E. Trotsenko
V. O. Kotova
L. A. Balakhontseva
L. V. Butakova
L. V. Varnavskaya
V. V. Fomenko
M. A. Chechulina
A. I. Krapivkin
S. V. Zinkovskaya
G. I. Budai
O. A. Yanovich
E. A. Lomakina
Z. I. Lukina
author_facet E. A. Bazykina
V. B. Turkutyukov
O. E. Trotsenko
V. O. Kotova
L. A. Balakhontseva
L. V. Butakova
L. V. Varnavskaya
V. V. Fomenko
M. A. Chechulina
A. I. Krapivkin
S. V. Zinkovskaya
G. I. Budai
O. A. Yanovich
E. A. Lomakina
Z. I. Lukina
author_sort E. A. Bazykina
title Prevalence and molecular-genetic characteristics of hepatitis B virus in HIV-positive individuals at the Far Eastern Federal District
title_short Prevalence and molecular-genetic characteristics of hepatitis B virus in HIV-positive individuals at the Far Eastern Federal District
title_full Prevalence and molecular-genetic characteristics of hepatitis B virus in HIV-positive individuals at the Far Eastern Federal District
title_fullStr Prevalence and molecular-genetic characteristics of hepatitis B virus in HIV-positive individuals at the Far Eastern Federal District
title_full_unstemmed Prevalence and molecular-genetic characteristics of hepatitis B virus in HIV-positive individuals at the Far Eastern Federal District
title_sort prevalence and molecular-genetic characteristics of hepatitis b virus in hiv-positive individuals at the far eastern federal district
publisher Sankt-Peterburg : NIIÈM imeni Pastera
publishDate 2019
url https://doaj.org/article/693ff6f20eef4e5288b49d4e3b461784
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