Conjugation Of Foot And Mouth Disease IgY In Chicken Egg Yolk With Horse Radish Peroxidase For Typing Of Foot And Mouth Disease Virus
Through the present work, anti-FMD IgY immunoglobulins for serotypes O; A, and SAT2 were prepared in chicken egg yolk through immunization of laying hens with locally prepared trivalent FMD vaccine. The antibody titres against three serotypes (O; A, and SAT2) measured by SNT were 2.58; 2.8 and 2.6 r...
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| Autores principales: | , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Egyptian Society for Animal Management
2017
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| Materias: | |
| Acceso en línea: | https://dx.doi.org/10.21608/javs.2017.65605 https://doaj.org/article/694c66ef4fa34e5b86e7e20cd43ce9a6 |
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| Sumario: | Through the present work, anti-FMD IgY immunoglobulins for serotypes O; A, and SAT2 were prepared in chicken egg yolk through immunization of laying hens with locally prepared trivalent FMD vaccine. The antibody titres against three serotypes (O; A, and SAT2) measured by SNT were 2.58; 2.8 and 2.6 respectively and where 2.81; 2.81 and 2.8 respectively when measured by ELISA. Evaluation of Anti-FMD IgY conjugated with Horse radish peroxidase for detection of FMD virus in 100 tongue epithelium; 50 OP fluid samples; 20 nasal swabs from naturally infected cattle, and 100 tissue culture infected fluids revealed sensitivity of 85% indicating the success of preparation of anti-FMD immunoglobulins conjugated with horse radish peroxidase as local product available on request saving cost and time. |
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