Conjugation Of Foot And Mouth Disease IgY In Chicken Egg Yolk With Horse Radish Peroxidase For Typing Of Foot And Mouth Disease Virus

Through the present work, anti-FMD IgY immunoglobulins for serotypes O; A, and SAT2 were prepared in chicken egg yolk through immunization of laying hens with locally prepared trivalent FMD vaccine. The antibody titres against three serotypes (O; A, and SAT2) measured by SNT were 2.58; 2.8 and 2.6 r...

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Autores principales: Ehab El-Sayed Ibrahim, Hamed Adel Hamed El-Helw, Nermen Guda Shafik, Manal Awad Mekhail
Formato: article
Lenguaje:EN
Publicado: Egyptian Society for Animal Management 2017
Materias:
fmd
pcr
Acceso en línea:https://dx.doi.org/10.21608/javs.2017.65605
https://doaj.org/article/694c66ef4fa34e5b86e7e20cd43ce9a6
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spelling oai:doaj.org-article:694c66ef4fa34e5b86e7e20cd43ce9a62021-12-02T11:44:35ZConjugation Of Foot And Mouth Disease IgY In Chicken Egg Yolk With Horse Radish Peroxidase For Typing Of Foot And Mouth Disease Virushttps://dx.doi.org/10.21608/javs.2017.656051687-40722090-3308https://doaj.org/article/694c66ef4fa34e5b86e7e20cd43ce9a62017-10-01T00:00:00Zhttps://javs.journals.ekb.eg/article_65605.htmlhttps://doaj.org/toc/1687-4072https://doaj.org/toc/2090-3308Through the present work, anti-FMD IgY immunoglobulins for serotypes O; A, and SAT2 were prepared in chicken egg yolk through immunization of laying hens with locally prepared trivalent FMD vaccine. The antibody titres against three serotypes (O; A, and SAT2) measured by SNT were 2.58; 2.8 and 2.6 respectively and where 2.81; 2.81 and 2.8 respectively when measured by ELISA. Evaluation of Anti-FMD IgY conjugated with Horse radish peroxidase for detection of FMD virus in 100 tongue epithelium; 50 OP fluid samples; 20 nasal swabs from naturally infected cattle, and 100 tissue culture infected fluids revealed sensitivity of 85% indicating the success of preparation of anti-FMD immunoglobulins conjugated with horse radish peroxidase as local product available on request saving cost and time.Ehab El-Sayed IbrahimHamed Adel Hamed El-HelwNermen Guda ShafikManal Awad MekhailEgyptian Society for Animal Managementarticleelisafmdimmunizationpcrhorse radish peroxidaseserotypesZoologyQL1-991Veterinary medicineSF600-1100Animal biochemistryQP501-801ENJournal of Applied Veterinary Sciences, Vol 2, Iss 1, Pp 35-42 (2017)
institution DOAJ
collection DOAJ
language EN
topic elisa
fmd
immunization
pcr
horse radish peroxidase
serotypes
Zoology
QL1-991
Veterinary medicine
SF600-1100
Animal biochemistry
QP501-801
spellingShingle elisa
fmd
immunization
pcr
horse radish peroxidase
serotypes
Zoology
QL1-991
Veterinary medicine
SF600-1100
Animal biochemistry
QP501-801
Ehab El-Sayed Ibrahim
Hamed Adel Hamed El-Helw
Nermen Guda Shafik
Manal Awad Mekhail
Conjugation Of Foot And Mouth Disease IgY In Chicken Egg Yolk With Horse Radish Peroxidase For Typing Of Foot And Mouth Disease Virus
description Through the present work, anti-FMD IgY immunoglobulins for serotypes O; A, and SAT2 were prepared in chicken egg yolk through immunization of laying hens with locally prepared trivalent FMD vaccine. The antibody titres against three serotypes (O; A, and SAT2) measured by SNT were 2.58; 2.8 and 2.6 respectively and where 2.81; 2.81 and 2.8 respectively when measured by ELISA. Evaluation of Anti-FMD IgY conjugated with Horse radish peroxidase for detection of FMD virus in 100 tongue epithelium; 50 OP fluid samples; 20 nasal swabs from naturally infected cattle, and 100 tissue culture infected fluids revealed sensitivity of 85% indicating the success of preparation of anti-FMD immunoglobulins conjugated with horse radish peroxidase as local product available on request saving cost and time.
format article
author Ehab El-Sayed Ibrahim
Hamed Adel Hamed El-Helw
Nermen Guda Shafik
Manal Awad Mekhail
author_facet Ehab El-Sayed Ibrahim
Hamed Adel Hamed El-Helw
Nermen Guda Shafik
Manal Awad Mekhail
author_sort Ehab El-Sayed Ibrahim
title Conjugation Of Foot And Mouth Disease IgY In Chicken Egg Yolk With Horse Radish Peroxidase For Typing Of Foot And Mouth Disease Virus
title_short Conjugation Of Foot And Mouth Disease IgY In Chicken Egg Yolk With Horse Radish Peroxidase For Typing Of Foot And Mouth Disease Virus
title_full Conjugation Of Foot And Mouth Disease IgY In Chicken Egg Yolk With Horse Radish Peroxidase For Typing Of Foot And Mouth Disease Virus
title_fullStr Conjugation Of Foot And Mouth Disease IgY In Chicken Egg Yolk With Horse Radish Peroxidase For Typing Of Foot And Mouth Disease Virus
title_full_unstemmed Conjugation Of Foot And Mouth Disease IgY In Chicken Egg Yolk With Horse Radish Peroxidase For Typing Of Foot And Mouth Disease Virus
title_sort conjugation of foot and mouth disease igy in chicken egg yolk with horse radish peroxidase for typing of foot and mouth disease virus
publisher Egyptian Society for Animal Management
publishDate 2017
url https://dx.doi.org/10.21608/javs.2017.65605
https://doaj.org/article/694c66ef4fa34e5b86e7e20cd43ce9a6
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