Conjugation Of Foot And Mouth Disease IgY In Chicken Egg Yolk With Horse Radish Peroxidase For Typing Of Foot And Mouth Disease Virus
Through the present work, anti-FMD IgY immunoglobulins for serotypes O; A, and SAT2 were prepared in chicken egg yolk through immunization of laying hens with locally prepared trivalent FMD vaccine. The antibody titres against three serotypes (O; A, and SAT2) measured by SNT were 2.58; 2.8 and 2.6 r...
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Egyptian Society for Animal Management
2017
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oai:doaj.org-article:694c66ef4fa34e5b86e7e20cd43ce9a62021-12-02T11:44:35ZConjugation Of Foot And Mouth Disease IgY In Chicken Egg Yolk With Horse Radish Peroxidase For Typing Of Foot And Mouth Disease Virushttps://dx.doi.org/10.21608/javs.2017.656051687-40722090-3308https://doaj.org/article/694c66ef4fa34e5b86e7e20cd43ce9a62017-10-01T00:00:00Zhttps://javs.journals.ekb.eg/article_65605.htmlhttps://doaj.org/toc/1687-4072https://doaj.org/toc/2090-3308Through the present work, anti-FMD IgY immunoglobulins for serotypes O; A, and SAT2 were prepared in chicken egg yolk through immunization of laying hens with locally prepared trivalent FMD vaccine. The antibody titres against three serotypes (O; A, and SAT2) measured by SNT were 2.58; 2.8 and 2.6 respectively and where 2.81; 2.81 and 2.8 respectively when measured by ELISA. Evaluation of Anti-FMD IgY conjugated with Horse radish peroxidase for detection of FMD virus in 100 tongue epithelium; 50 OP fluid samples; 20 nasal swabs from naturally infected cattle, and 100 tissue culture infected fluids revealed sensitivity of 85% indicating the success of preparation of anti-FMD immunoglobulins conjugated with horse radish peroxidase as local product available on request saving cost and time.Ehab El-Sayed IbrahimHamed Adel Hamed El-HelwNermen Guda ShafikManal Awad MekhailEgyptian Society for Animal Managementarticleelisafmdimmunizationpcrhorse radish peroxidaseserotypesZoologyQL1-991Veterinary medicineSF600-1100Animal biochemistryQP501-801ENJournal of Applied Veterinary Sciences, Vol 2, Iss 1, Pp 35-42 (2017) |
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elisa fmd immunization pcr horse radish peroxidase serotypes Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801 |
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elisa fmd immunization pcr horse radish peroxidase serotypes Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801 Ehab El-Sayed Ibrahim Hamed Adel Hamed El-Helw Nermen Guda Shafik Manal Awad Mekhail Conjugation Of Foot And Mouth Disease IgY In Chicken Egg Yolk With Horse Radish Peroxidase For Typing Of Foot And Mouth Disease Virus |
description |
Through the present work, anti-FMD IgY immunoglobulins for serotypes O; A, and SAT2 were prepared in chicken egg yolk through immunization of laying hens with locally prepared trivalent FMD vaccine. The antibody titres against three serotypes (O; A, and SAT2) measured by SNT were 2.58; 2.8 and 2.6 respectively and where 2.81; 2.81 and 2.8 respectively when measured by ELISA. Evaluation of Anti-FMD IgY conjugated with Horse radish peroxidase for detection of FMD virus in 100 tongue epithelium; 50 OP fluid samples; 20 nasal swabs from naturally infected cattle, and 100 tissue culture infected fluids revealed sensitivity of 85% indicating the success of preparation of anti-FMD immunoglobulins conjugated with horse radish peroxidase as local product available on request saving cost and time. |
format |
article |
author |
Ehab El-Sayed Ibrahim Hamed Adel Hamed El-Helw Nermen Guda Shafik Manal Awad Mekhail |
author_facet |
Ehab El-Sayed Ibrahim Hamed Adel Hamed El-Helw Nermen Guda Shafik Manal Awad Mekhail |
author_sort |
Ehab El-Sayed Ibrahim |
title |
Conjugation Of Foot And Mouth Disease IgY In Chicken Egg Yolk With Horse Radish Peroxidase For Typing Of Foot And Mouth Disease Virus |
title_short |
Conjugation Of Foot And Mouth Disease IgY In Chicken Egg Yolk With Horse Radish Peroxidase For Typing Of Foot And Mouth Disease Virus |
title_full |
Conjugation Of Foot And Mouth Disease IgY In Chicken Egg Yolk With Horse Radish Peroxidase For Typing Of Foot And Mouth Disease Virus |
title_fullStr |
Conjugation Of Foot And Mouth Disease IgY In Chicken Egg Yolk With Horse Radish Peroxidase For Typing Of Foot And Mouth Disease Virus |
title_full_unstemmed |
Conjugation Of Foot And Mouth Disease IgY In Chicken Egg Yolk With Horse Radish Peroxidase For Typing Of Foot And Mouth Disease Virus |
title_sort |
conjugation of foot and mouth disease igy in chicken egg yolk with horse radish peroxidase for typing of foot and mouth disease virus |
publisher |
Egyptian Society for Animal Management |
publishDate |
2017 |
url |
https://dx.doi.org/10.21608/javs.2017.65605 https://doaj.org/article/694c66ef4fa34e5b86e7e20cd43ce9a6 |
work_keys_str_mv |
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1718395284903428096 |