Knockdown of circular RNA septin 9 inhibits the malignant progression of breast cancer by reducing the expression of solute carrier family 1 member 5 in a microRNA-149-5p-dependent manner

Breast cancer (BC) is the most frequently diagnosed cancer in women. Increasing evidence suggests that circular RNA (circRNA) exerts critical functions in BC progression. However, the roles of circRNA septin 9 (circSEPT9) in BC development and the underneath mechanism remain largely unclear so far....

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jianjun Wang, Kunxian Yang, Junyu Cao, Li Li
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	breast cancer circsept9 mir-149-5p slc1a5 Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/694c7eebdf444a72826e43ea2bf2056f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:694c7eebdf444a72826e43ea2bf2056f
record_format	dspace
spelling	oai:doaj.org-article:694c7eebdf444a72826e43ea2bf2056f2021-11-11T14:23:43ZKnockdown of circular RNA septin 9 inhibits the malignant progression of breast cancer by reducing the expression of solute carrier family 1 member 5 in a microRNA-149-5p-dependent manner2165-59792165-598710.1080/21655979.2021.2000731https://doaj.org/article/694c7eebdf444a72826e43ea2bf2056f2021-11-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.2000731https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987Breast cancer (BC) is the most frequently diagnosed cancer in women. Increasing evidence suggests that circular RNA (circRNA) exerts critical functions in BC progression. However, the roles of circRNA septin 9 (circSEPT9) in BC development and the underneath mechanism remain largely unclear so far. In this work, the RNA levels of circSEPT9, microRNA-149-5p (miR-149-5p) and solute carrier family 1 member 5 (SLC1A5) were detected by quantitative real-time polymerase chain reaction. Western blot was performed to check protein expression. Glutamine uptake, cell proliferation and cell apoptosis were investigated by glutamine uptake, cell counting kit-8, cell colony formation, 5-Ethynyl-29-deoxyuridine, flow cytometry analysis or DNA content quantitation assay. The interactions of miR-149-5p with circSEPT9 and SLC1A5 were identified by a dual-luciferase reporter assay. Mouse model assay was carried out to analyze the effect of circSEPT9 on tumor formation in vivo. Results showed that circSEPT9 and SLC1A5 expression were significantly upregulated, while miR-149-5p was downregulated in BC tissues and cells as compared with paracancerous normal breast tissues and human normal breast cells. Knockdown of circSEPT9 or SLC1A5 inhibited glutamine uptake and cell proliferation, but induced cell apoptosis in BC cells. SLC1A5 overexpression relieved circSEPT9 silencing-induced repression of BC cell malignancy. In mechanism, circSEPT9 regulated SLC1A5 expression by sponging miR-149-5p. In support, circSEPT9 knockdown led to delayed tumor tumorigenesis in vivo. In summary, these results indicates that circSEPT9 may act an oncogenic role in BC malignant progression by regulating miR-149-5p/SLC1A5 pathway, providing a novel mechanism responsible for BC development.Jianjun WangKunxian YangJunyu CaoLi LiTaylor & Francis Grouparticlebreast cancercircsept9mir-149-5pslc1a5BiotechnologyTP248.13-248.65ENBioengineered, Vol 0, Iss 0 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	breast cancer circsept9 mir-149-5p slc1a5 Biotechnology TP248.13-248.65
spellingShingle	breast cancer circsept9 mir-149-5p slc1a5 Biotechnology TP248.13-248.65 Jianjun Wang Kunxian Yang Junyu Cao Li Li Knockdown of circular RNA septin 9 inhibits the malignant progression of breast cancer by reducing the expression of solute carrier family 1 member 5 in a microRNA-149-5p-dependent manner
description	Breast cancer (BC) is the most frequently diagnosed cancer in women. Increasing evidence suggests that circular RNA (circRNA) exerts critical functions in BC progression. However, the roles of circRNA septin 9 (circSEPT9) in BC development and the underneath mechanism remain largely unclear so far. In this work, the RNA levels of circSEPT9, microRNA-149-5p (miR-149-5p) and solute carrier family 1 member 5 (SLC1A5) were detected by quantitative real-time polymerase chain reaction. Western blot was performed to check protein expression. Glutamine uptake, cell proliferation and cell apoptosis were investigated by glutamine uptake, cell counting kit-8, cell colony formation, 5-Ethynyl-29-deoxyuridine, flow cytometry analysis or DNA content quantitation assay. The interactions of miR-149-5p with circSEPT9 and SLC1A5 were identified by a dual-luciferase reporter assay. Mouse model assay was carried out to analyze the effect of circSEPT9 on tumor formation in vivo. Results showed that circSEPT9 and SLC1A5 expression were significantly upregulated, while miR-149-5p was downregulated in BC tissues and cells as compared with paracancerous normal breast tissues and human normal breast cells. Knockdown of circSEPT9 or SLC1A5 inhibited glutamine uptake and cell proliferation, but induced cell apoptosis in BC cells. SLC1A5 overexpression relieved circSEPT9 silencing-induced repression of BC cell malignancy. In mechanism, circSEPT9 regulated SLC1A5 expression by sponging miR-149-5p. In support, circSEPT9 knockdown led to delayed tumor tumorigenesis in vivo. In summary, these results indicates that circSEPT9 may act an oncogenic role in BC malignant progression by regulating miR-149-5p/SLC1A5 pathway, providing a novel mechanism responsible for BC development.
format	article
author	Jianjun Wang Kunxian Yang Junyu Cao Li Li
author_facet	Jianjun Wang Kunxian Yang Junyu Cao Li Li
author_sort	Jianjun Wang
title	Knockdown of circular RNA septin 9 inhibits the malignant progression of breast cancer by reducing the expression of solute carrier family 1 member 5 in a microRNA-149-5p-dependent manner
title_short	Knockdown of circular RNA septin 9 inhibits the malignant progression of breast cancer by reducing the expression of solute carrier family 1 member 5 in a microRNA-149-5p-dependent manner
title_full	Knockdown of circular RNA septin 9 inhibits the malignant progression of breast cancer by reducing the expression of solute carrier family 1 member 5 in a microRNA-149-5p-dependent manner
title_fullStr	Knockdown of circular RNA septin 9 inhibits the malignant progression of breast cancer by reducing the expression of solute carrier family 1 member 5 in a microRNA-149-5p-dependent manner
title_full_unstemmed	Knockdown of circular RNA septin 9 inhibits the malignant progression of breast cancer by reducing the expression of solute carrier family 1 member 5 in a microRNA-149-5p-dependent manner
title_sort	knockdown of circular rna septin 9 inhibits the malignant progression of breast cancer by reducing the expression of solute carrier family 1 member 5 in a microrna-149-5p-dependent manner
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/694c7eebdf444a72826e43ea2bf2056f
work_keys_str_mv	AT jianjunwang knockdownofcircularrnaseptin9inhibitsthemalignantprogressionofbreastcancerbyreducingtheexpressionofsolutecarrierfamily1member5inamicrorna1495pdependentmanner AT kunxianyang knockdownofcircularrnaseptin9inhibitsthemalignantprogressionofbreastcancerbyreducingtheexpressionofsolutecarrierfamily1member5inamicrorna1495pdependentmanner AT junyucao knockdownofcircularrnaseptin9inhibitsthemalignantprogressionofbreastcancerbyreducingtheexpressionofsolutecarrierfamily1member5inamicrorna1495pdependentmanner AT lili knockdownofcircularrnaseptin9inhibitsthemalignantprogressionofbreastcancerbyreducingtheexpressionofsolutecarrierfamily1member5inamicrorna1495pdependentmanner
_version_	1718438942221533184

Knockdown of circular RNA septin 9 inhibits the malignant progression of breast cancer by reducing the expression of solute carrier family 1 member 5 in a microRNA-149-5p-dependent manner

Ejemplares similares