Antigenic And Genomic Characterization Of Local Fowlpox Virus Isolate In 2017

This study presents a local isolate of fowl pox virus (FPV) isolated and propagated from backyard naturally infected laying hens in El-Sharkia Governorate, Egypt, during the period of January to November 2017. Isolation and propagation were carried out from collected skin lesions on the chorioallant...

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Autores principales:	Aboul Soud, E. A., Ayatollah, I. Ibrahim., Abd El-Moaty, D. A. M., Kafafy M. H., Abass. A. M.
Formato:	article
Lenguaje:	EN
Publicado:	Egyptian Society for Animal Management 2020
Materias:	fowlpox virus (fpv) virus neutralization test (vnt) p4b (fpv167) fpv140 Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801
Acceso en línea:	https://dx.doi.org/10.21608/javs.2020.98319 https://doaj.org/article/6954aacd51624fbfb5c973a5b3330822
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spelling	oai:doaj.org-article:6954aacd51624fbfb5c973a5b33308222021-12-02T17:04:24ZAntigenic And Genomic Characterization Of Local Fowlpox Virus Isolate In 2017https://dx.doi.org/10.21608/javs.2020.983191687-40722090-3308https://doaj.org/article/6954aacd51624fbfb5c973a5b33308222020-07-01T00:00:00Zhttps://javs.journals.ekb.eg/article_98319.htmlhttps://doaj.org/toc/1687-4072https://doaj.org/toc/2090-3308This study presents a local isolate of fowl pox virus (FPV) isolated and propagated from backyard naturally infected laying hens in El-Sharkia Governorate, Egypt, during the period of January to November 2017. Isolation and propagation were carried out from collected skin lesions on the chorioallantoic membrane (CAM) and chicken embryo fibroblast (CEF) with obtained 4th passage virus titers 4.0 Log10 EID50/ml at the in CAM and 3.5 Log10 TCID50/ml in CEF respectively. They showed characteristic pock lesions of FPV and cytopathic effect (CPE) of FPV at the 3rd passage on CAM and CEF, respectively. Virus neutralization test (VNT) results confirmed that the obtained isolate is FPV. Molecular characterization of (Sharkia2017/VSVRI) was performed with Polymerase chain reaction (PCR) to amplify 578 bp of P4b (fpv167) gene and 1150 bp of fpv140 gene. Sequence and phylogenetic analysis of both genes confirmed the relatedness of (Sharkia2017/VSVRI) isolate to sub-clade A1 of fowl pox viruses with 99.7-100% identity to fowl pox virus sequences published in GenBank.This study reports the antigenic and genomic characterization of the locally isolated FPV (Sharkia2017/VSVRI) using VNT and PCR confirmed by sequence analysis to help in the production of FPV tissue culture vaccine from the obtained local FPV after confirming its immune response as a candidate vaccine.Aboul Soud, E. A.Ayatollah, I. Ibrahim.Abd El-Moaty, D. A. M. Kafafy M. H.Abass. A. M.Egyptian Society for Animal Managementarticlefowlpox virus (fpv)virus neutralization test (vnt)p4b (fpv167)fpv140ZoologyQL1-991Veterinary medicineSF600-1100Animal biochemistryQP501-801ENJournal of Applied Veterinary Sciences, Vol 5, Iss 3, Pp 31-39 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	fowlpox virus (fpv) virus neutralization test (vnt) p4b (fpv167) fpv140 Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801
spellingShingle	fowlpox virus (fpv) virus neutralization test (vnt) p4b (fpv167) fpv140 Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801 Aboul Soud, E. A. Ayatollah, I. Ibrahim. Abd El-Moaty, D. A. M. Kafafy M. H. Abass. A. M. Antigenic And Genomic Characterization Of Local Fowlpox Virus Isolate In 2017
description	This study presents a local isolate of fowl pox virus (FPV) isolated and propagated from backyard naturally infected laying hens in El-Sharkia Governorate, Egypt, during the period of January to November 2017. Isolation and propagation were carried out from collected skin lesions on the chorioallantoic membrane (CAM) and chicken embryo fibroblast (CEF) with obtained 4th passage virus titers 4.0 Log10 EID50/ml at the in CAM and 3.5 Log10 TCID50/ml in CEF respectively. They showed characteristic pock lesions of FPV and cytopathic effect (CPE) of FPV at the 3rd passage on CAM and CEF, respectively. Virus neutralization test (VNT) results confirmed that the obtained isolate is FPV. Molecular characterization of (Sharkia2017/VSVRI) was performed with Polymerase chain reaction (PCR) to amplify 578 bp of P4b (fpv167) gene and 1150 bp of fpv140 gene. Sequence and phylogenetic analysis of both genes confirmed the relatedness of (Sharkia2017/VSVRI) isolate to sub-clade A1 of fowl pox viruses with 99.7-100% identity to fowl pox virus sequences published in GenBank.This study reports the antigenic and genomic characterization of the locally isolated FPV (Sharkia2017/VSVRI) using VNT and PCR confirmed by sequence analysis to help in the production of FPV tissue culture vaccine from the obtained local FPV after confirming its immune response as a candidate vaccine.
format	article
author	Aboul Soud, E. A. Ayatollah, I. Ibrahim. Abd El-Moaty, D. A. M. Kafafy M. H. Abass. A. M.
author_facet	Aboul Soud, E. A. Ayatollah, I. Ibrahim. Abd El-Moaty, D. A. M. Kafafy M. H. Abass. A. M.
author_sort	Aboul Soud, E. A.
title	Antigenic And Genomic Characterization Of Local Fowlpox Virus Isolate In 2017
title_short	Antigenic And Genomic Characterization Of Local Fowlpox Virus Isolate In 2017
title_full	Antigenic And Genomic Characterization Of Local Fowlpox Virus Isolate In 2017
title_fullStr	Antigenic And Genomic Characterization Of Local Fowlpox Virus Isolate In 2017
title_full_unstemmed	Antigenic And Genomic Characterization Of Local Fowlpox Virus Isolate In 2017
title_sort	antigenic and genomic characterization of local fowlpox virus isolate in 2017
publisher	Egyptian Society for Animal Management
publishDate	2020
url	https://dx.doi.org/10.21608/javs.2020.98319 https://doaj.org/article/6954aacd51624fbfb5c973a5b3330822
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_version_	1718381853775233024

Antigenic And Genomic Characterization Of Local Fowlpox Virus Isolate In 2017

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