High-throughput RNA sequencing of paraformaldehyde-fixed single cells

Current high-throughput single-cell transcriptomic methods are incompatible with paraformaldehyde, a common cell fixation technique. Here the authors present FD-seq, a method for droplet-based RNA sequencing of paraformaldehyde-fixed, stained and sorted single cells.

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Autores principales:	Hoang Van Phan, Michiel van Gent, Nir Drayman, Anindita Basu, Michaela U. Gack, Savaş Tay
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/6966a809fe76487f8ea1bce4ce5cfb20
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spelling	oai:doaj.org-article:6966a809fe76487f8ea1bce4ce5cfb202021-12-02T15:15:14ZHigh-throughput RNA sequencing of paraformaldehyde-fixed single cells10.1038/s41467-021-25871-22041-1723https://doaj.org/article/6966a809fe76487f8ea1bce4ce5cfb202021-09-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-25871-2https://doaj.org/toc/2041-1723Current high-throughput single-cell transcriptomic methods are incompatible with paraformaldehyde, a common cell fixation technique. Here the authors present FD-seq, a method for droplet-based RNA sequencing of paraformaldehyde-fixed, stained and sorted single cells.Hoang Van PhanMichiel van GentNir DraymanAnindita BasuMichaela U. GackSavaş TayNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Hoang Van Phan Michiel van Gent Nir Drayman Anindita Basu Michaela U. Gack Savaş Tay High-throughput RNA sequencing of paraformaldehyde-fixed single cells
description	Current high-throughput single-cell transcriptomic methods are incompatible with paraformaldehyde, a common cell fixation technique. Here the authors present FD-seq, a method for droplet-based RNA sequencing of paraformaldehyde-fixed, stained and sorted single cells.
format	article
author	Hoang Van Phan Michiel van Gent Nir Drayman Anindita Basu Michaela U. Gack Savaş Tay
author_facet	Hoang Van Phan Michiel van Gent Nir Drayman Anindita Basu Michaela U. Gack Savaş Tay
author_sort	Hoang Van Phan
title	High-throughput RNA sequencing of paraformaldehyde-fixed single cells
title_short	High-throughput RNA sequencing of paraformaldehyde-fixed single cells
title_full	High-throughput RNA sequencing of paraformaldehyde-fixed single cells
title_fullStr	High-throughput RNA sequencing of paraformaldehyde-fixed single cells
title_full_unstemmed	High-throughput RNA sequencing of paraformaldehyde-fixed single cells
title_sort	high-throughput rna sequencing of paraformaldehyde-fixed single cells
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/6966a809fe76487f8ea1bce4ce5cfb20
work_keys_str_mv	AT hoangvanphan highthroughputrnasequencingofparaformaldehydefixedsinglecells AT michielvangent highthroughputrnasequencingofparaformaldehydefixedsinglecells AT nirdrayman highthroughputrnasequencingofparaformaldehydefixedsinglecells AT aninditabasu highthroughputrnasequencingofparaformaldehydefixedsinglecells AT michaelaugack highthroughputrnasequencingofparaformaldehydefixedsinglecells AT savastay highthroughputrnasequencingofparaformaldehydefixedsinglecells
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High-throughput RNA sequencing of paraformaldehyde-fixed single cells

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