High-throughput RNA sequencing of paraformaldehyde-fixed single cells
Current high-throughput single-cell transcriptomic methods are incompatible with paraformaldehyde, a common cell fixation technique. Here the authors present FD-seq, a method for droplet-based RNA sequencing of paraformaldehyde-fixed, stained and sorted single cells.
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| Main Authors: | Hoang Van Phan, Michiel van Gent, Nir Drayman, Anindita Basu, Michaela U. Gack, Savaş Tay |
|---|---|
| Format: | article |
| Language: | EN |
| Published: |
Nature Portfolio
2021
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| Subjects: | |
| Online Access: | https://doaj.org/article/6966a809fe76487f8ea1bce4ce5cfb20 |
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