High-throughput RNA sequencing of paraformaldehyde-fixed single cells

High-throughput RNA sequencing of paraformaldehyde-fixed single cells

Current high-throughput single-cell transcriptomic methods are incompatible with paraformaldehyde, a common cell fixation technique. Here the authors present FD-seq, a method for droplet-based RNA sequencing of paraformaldehyde-fixed, stained and sorted single cells.

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Autores principales: Hoang Van Phan, Michiel van Gent, Nir Drayman, Anindita Basu, Michaela U. Gack, Savaş Tay
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
Materias:
Science
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Acceso en línea:https://doaj.org/article/6966a809fe76487f8ea1bce4ce5cfb20
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