Phylogenomic analysis of MKKs and MAPKs from 16 legumes and detection of interacting pairs in chickpea divulge MAPK signalling modules

Abstract The mitogen-activated protein kinase (MAPK)-mediated phosphorylation cascade is a vital component of plant cellular signalling. Despite this, MAPK signalling cascade is less characterized in crop legumes. To fill this void, we present here a comprehensive phylogeny of MAPK kinases (MKKs) an...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Savithri Purayannur, Kamal Kumar, Vemula Chandra Kaladhar, Praveen Kumar Verma
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6971f18bd6cd4726aeb026826024e0f4
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:6971f18bd6cd4726aeb026826024e0f4
record_format	dspace
spelling	oai:doaj.org-article:6971f18bd6cd4726aeb026826024e0f42021-12-02T11:52:30ZPhylogenomic analysis of MKKs and MAPKs from 16 legumes and detection of interacting pairs in chickpea divulge MAPK signalling modules10.1038/s41598-017-04913-02045-2322https://doaj.org/article/6971f18bd6cd4726aeb026826024e0f42017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04913-0https://doaj.org/toc/2045-2322Abstract The mitogen-activated protein kinase (MAPK)-mediated phosphorylation cascade is a vital component of plant cellular signalling. Despite this, MAPK signalling cascade is less characterized in crop legumes. To fill this void, we present here a comprehensive phylogeny of MAPK kinases (MKKs) and MAPKs identified from 16 legume species belonging to genistoid (Lupinus angustifolius), dalbergioid (Arachis spp.), phaseoloid (Glycine max, Cajanus cajan, Phaseolus vulgaris, and Vigna spp.), and galegoid (Cicer arietinum, Lotus japonicus, Medicago truncatula, Pisum sativum, Trifolium spp., and Vicia faba) clades. Using the genes of the diploid crop chickpea (C. arietinum), an exhaustive interaction analysis was performed between MKKs and MAPKs by split-ubiquitin based yeast two-hybrid (Y2H). Twenty seven interactions of varying strengths were identified between chickpea MKKs and MAPKs. These interactions were verified in planta by bimolecular fluorescence complementation (BiFC). As a first report in plants, four intra-molecular interactions of weak strength were identified within chickpea MKKs. Additionally; two TEOSINTE-BRANCHED1/CYCLOIDEA/PCF (TCP) transcription factors of class I were identified as novel down-stream interacting partners of seven MAPKs. We propose that this highly reliable MAPK interaction network, presented here for chickpea, can be utilized as a reference for legumes and thus will help in deciphering their role in legume-specific events.Savithri PurayannurKamal KumarVemula Chandra KaladharPraveen Kumar VermaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Savithri Purayannur Kamal Kumar Vemula Chandra Kaladhar Praveen Kumar Verma Phylogenomic analysis of MKKs and MAPKs from 16 legumes and detection of interacting pairs in chickpea divulge MAPK signalling modules
description	Abstract The mitogen-activated protein kinase (MAPK)-mediated phosphorylation cascade is a vital component of plant cellular signalling. Despite this, MAPK signalling cascade is less characterized in crop legumes. To fill this void, we present here a comprehensive phylogeny of MAPK kinases (MKKs) and MAPKs identified from 16 legume species belonging to genistoid (Lupinus angustifolius), dalbergioid (Arachis spp.), phaseoloid (Glycine max, Cajanus cajan, Phaseolus vulgaris, and Vigna spp.), and galegoid (Cicer arietinum, Lotus japonicus, Medicago truncatula, Pisum sativum, Trifolium spp., and Vicia faba) clades. Using the genes of the diploid crop chickpea (C. arietinum), an exhaustive interaction analysis was performed between MKKs and MAPKs by split-ubiquitin based yeast two-hybrid (Y2H). Twenty seven interactions of varying strengths were identified between chickpea MKKs and MAPKs. These interactions were verified in planta by bimolecular fluorescence complementation (BiFC). As a first report in plants, four intra-molecular interactions of weak strength were identified within chickpea MKKs. Additionally; two TEOSINTE-BRANCHED1/CYCLOIDEA/PCF (TCP) transcription factors of class I were identified as novel down-stream interacting partners of seven MAPKs. We propose that this highly reliable MAPK interaction network, presented here for chickpea, can be utilized as a reference for legumes and thus will help in deciphering their role in legume-specific events.
format	article
author	Savithri Purayannur Kamal Kumar Vemula Chandra Kaladhar Praveen Kumar Verma
author_facet	Savithri Purayannur Kamal Kumar Vemula Chandra Kaladhar Praveen Kumar Verma
author_sort	Savithri Purayannur
title	Phylogenomic analysis of MKKs and MAPKs from 16 legumes and detection of interacting pairs in chickpea divulge MAPK signalling modules
title_short	Phylogenomic analysis of MKKs and MAPKs from 16 legumes and detection of interacting pairs in chickpea divulge MAPK signalling modules
title_full	Phylogenomic analysis of MKKs and MAPKs from 16 legumes and detection of interacting pairs in chickpea divulge MAPK signalling modules
title_fullStr	Phylogenomic analysis of MKKs and MAPKs from 16 legumes and detection of interacting pairs in chickpea divulge MAPK signalling modules
title_full_unstemmed	Phylogenomic analysis of MKKs and MAPKs from 16 legumes and detection of interacting pairs in chickpea divulge MAPK signalling modules
title_sort	phylogenomic analysis of mkks and mapks from 16 legumes and detection of interacting pairs in chickpea divulge mapk signalling modules
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/6971f18bd6cd4726aeb026826024e0f4
work_keys_str_mv	AT savithripurayannur phylogenomicanalysisofmkksandmapksfrom16legumesanddetectionofinteractingpairsinchickpeadivulgemapksignallingmodules AT kamalkumar phylogenomicanalysisofmkksandmapksfrom16legumesanddetectionofinteractingpairsinchickpeadivulgemapksignallingmodules AT vemulachandrakaladhar phylogenomicanalysisofmkksandmapksfrom16legumesanddetectionofinteractingpairsinchickpeadivulgemapksignallingmodules AT praveenkumarverma phylogenomicanalysisofmkksandmapksfrom16legumesanddetectionofinteractingpairsinchickpeadivulgemapksignallingmodules
_version_	1718395050421911552

Phylogenomic analysis of MKKs and MAPKs from 16 legumes and detection of interacting pairs in chickpea divulge MAPK signalling modules

Ejemplares similares