POLYMORPHISMS OF EXTRACELLULAR CONNECTIVE TISSUE REMODELING PROTEINASES AND <i>MMP2, MMP3, MMP9</i> GENES, AND NEOANGIGENESIS <i>VEGF</i> GENE IN RETINAL MICROANGIOPATHY IN THE PATIENTS WITH TYPE 2 DIABETES MELLITUS

The aim of our study was to perform an association analysis between MMP2, MMP3, MMP9, VEGF gene polymorphisms and development of non-proliferative diabetic retinopathy (DR) in the type 2 diabetic patients (DM).201 DM patients: 90 cases of DR and 111 subjects without DR features were included into th...

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Autores principales:	A. V. Shevchenko, V. F. Prokofiev, V. I. Konenkov, V. V. Klimontov, N. V. Tyan, D. V. Chernykh, A. N. Trunov, V. V. Chernykh
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2019
Materias:	diabetic retinopathy matrix metalloproteinases genes vascular endothelial growth factor gene genic networks mathematical modelling Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/697b641fb60b4d19942ca7d37364493d
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spelling	oai:doaj.org-article:697b641fb60b4d19942ca7d37364493d2021-11-18T08:03:48ZPOLYMORPHISMS OF EXTRACELLULAR CONNECTIVE TISSUE REMODELING PROTEINASES AND <i>MMP2, MMP3, MMP9</i> GENES, AND NEOANGIGENESIS <i>VEGF</i> GENE IN RETINAL MICROANGIOPATHY IN THE PATIENTS WITH TYPE 2 DIABETES MELLITUS1563-06252313-741X10.15789/1563-0625-2019-3-441-450https://doaj.org/article/697b641fb60b4d19942ca7d37364493d2019-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/1817https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XThe aim of our study was to perform an association analysis between MMP2, MMP3, MMP9, VEGF gene polymorphisms and development of non-proliferative diabetic retinopathy (DR) in the type 2 diabetic patients (DM).201 DM patients: 90 cases of DR and 111 subjects without DR features were included into the study. Polymorphic variants of MMP2 (rs2438650), MMP3 (rs3025058), MMP9 (rs3918242), and VEGF (rs699947 and rs3025039) genes were assayed. The genetic typing was carried out by restriction fragment length polymorphism and TaqMan methods.The analysis of complex genotypes at the five polymorphic positions has revealed some significant findings in positive and negatively incorporated complexes. Increased frequencies of MMP2-1306 CC genotype in the group of patients with “early” development of complication, and more frequent combination of high-level HbA1c with MMP2-1306CC and MMP9-1562CT genotypes were shown in DR patients. Computerassisted modelling with visual reconstruction of network interactions between the genotypes involved into the destruction events and angiogenesis, as well as altered HbA1с levels (an integral parameter of glycemia), has revealed some differences in structural and functional organization of gene-gene and gene- protein interactions between the groups of patients with DR versus those without this disorder. Сonclusion. A design of interactome biological networks based on transcription regulation and metabolic pathways, as well as their topological analysis allows to build and study interactions of genes and proteins, with reference to pathogenetic studies of DM2 complications aiming for development of approaches to personalized prevention and therapy in future times.A. V. ShevchenkoV. F. ProkofievV. I. KonenkovV. V. KlimontovN. V. TyanD. V. ChernykhA. N. TrunovV. V. ChernykhSPb RAACIarticlediabetic retinopathymatrix metalloproteinases genesvascular endothelial growth factor genegenic networksmathematical modellingImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 21, Iss 3, Pp 441-450 (2019)
institution	DOAJ
collection	DOAJ
language	RU
topic	diabetic retinopathy matrix metalloproteinases genes vascular endothelial growth factor gene genic networks mathematical modelling Immunologic diseases. Allergy RC581-607
spellingShingle	diabetic retinopathy matrix metalloproteinases genes vascular endothelial growth factor gene genic networks mathematical modelling Immunologic diseases. Allergy RC581-607 A. V. Shevchenko V. F. Prokofiev V. I. Konenkov V. V. Klimontov N. V. Tyan D. V. Chernykh A. N. Trunov V. V. Chernykh POLYMORPHISMS OF EXTRACELLULAR CONNECTIVE TISSUE REMODELING PROTEINASES AND <i>MMP2, MMP3, MMP9</i> GENES, AND NEOANGIGENESIS <i>VEGF</i> GENE IN RETINAL MICROANGIOPATHY IN THE PATIENTS WITH TYPE 2 DIABETES MELLITUS
description	The aim of our study was to perform an association analysis between MMP2, MMP3, MMP9, VEGF gene polymorphisms and development of non-proliferative diabetic retinopathy (DR) in the type 2 diabetic patients (DM).201 DM patients: 90 cases of DR and 111 subjects without DR features were included into the study. Polymorphic variants of MMP2 (rs2438650), MMP3 (rs3025058), MMP9 (rs3918242), and VEGF (rs699947 and rs3025039) genes were assayed. The genetic typing was carried out by restriction fragment length polymorphism and TaqMan methods.The analysis of complex genotypes at the five polymorphic positions has revealed some significant findings in positive and negatively incorporated complexes. Increased frequencies of MMP2-1306 CC genotype in the group of patients with “early” development of complication, and more frequent combination of high-level HbA1c with MMP2-1306CC and MMP9-1562CT genotypes were shown in DR patients. Computerassisted modelling with visual reconstruction of network interactions between the genotypes involved into the destruction events and angiogenesis, as well as altered HbA1с levels (an integral parameter of glycemia), has revealed some differences in structural and functional organization of gene-gene and gene- protein interactions between the groups of patients with DR versus those without this disorder. Сonclusion. A design of interactome biological networks based on transcription regulation and metabolic pathways, as well as their topological analysis allows to build and study interactions of genes and proteins, with reference to pathogenetic studies of DM2 complications aiming for development of approaches to personalized prevention and therapy in future times.
format	article
author	A. V. Shevchenko V. F. Prokofiev V. I. Konenkov V. V. Klimontov N. V. Tyan D. V. Chernykh A. N. Trunov V. V. Chernykh
author_facet	A. V. Shevchenko V. F. Prokofiev V. I. Konenkov V. V. Klimontov N. V. Tyan D. V. Chernykh A. N. Trunov V. V. Chernykh
author_sort	A. V. Shevchenko
title	POLYMORPHISMS OF EXTRACELLULAR CONNECTIVE TISSUE REMODELING PROTEINASES AND <i>MMP2, MMP3, MMP9</i> GENES, AND NEOANGIGENESIS <i>VEGF</i> GENE IN RETINAL MICROANGIOPATHY IN THE PATIENTS WITH TYPE 2 DIABETES MELLITUS
title_short	POLYMORPHISMS OF EXTRACELLULAR CONNECTIVE TISSUE REMODELING PROTEINASES AND <i>MMP2, MMP3, MMP9</i> GENES, AND NEOANGIGENESIS <i>VEGF</i> GENE IN RETINAL MICROANGIOPATHY IN THE PATIENTS WITH TYPE 2 DIABETES MELLITUS
title_full	POLYMORPHISMS OF EXTRACELLULAR CONNECTIVE TISSUE REMODELING PROTEINASES AND <i>MMP2, MMP3, MMP9</i> GENES, AND NEOANGIGENESIS <i>VEGF</i> GENE IN RETINAL MICROANGIOPATHY IN THE PATIENTS WITH TYPE 2 DIABETES MELLITUS
title_fullStr	POLYMORPHISMS OF EXTRACELLULAR CONNECTIVE TISSUE REMODELING PROTEINASES AND <i>MMP2, MMP3, MMP9</i> GENES, AND NEOANGIGENESIS <i>VEGF</i> GENE IN RETINAL MICROANGIOPATHY IN THE PATIENTS WITH TYPE 2 DIABETES MELLITUS
title_full_unstemmed	POLYMORPHISMS OF EXTRACELLULAR CONNECTIVE TISSUE REMODELING PROTEINASES AND <i>MMP2, MMP3, MMP9</i> GENES, AND NEOANGIGENESIS <i>VEGF</i> GENE IN RETINAL MICROANGIOPATHY IN THE PATIENTS WITH TYPE 2 DIABETES MELLITUS
title_sort	polymorphisms of extracellular connective tissue remodeling proteinases and <i>mmp2, mmp3, mmp9</i> genes, and neoangigenesis <i>vegf</i> gene in retinal microangiopathy in the patients with type 2 diabetes mellitus
publisher	SPb RAACI
publishDate	2019
url	https://doaj.org/article/697b641fb60b4d19942ca7d37364493d
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_version_	1718422356829929472

POLYMORPHISMS OF EXTRACELLULAR CONNECTIVE TISSUE REMODELING PROTEINASES AND <i>MMP2, MMP3, MMP9</i> GENES, AND NEOANGIGENESIS <i>VEGF</i> GENE IN RETINAL MICROANGIOPATHY IN THE PATIENTS WITH TYPE 2 DIABETES MELLITUS

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