EFFICIENCY OF DIFFERENT TECHNIQUES FOR ANTIBODY DETERMINATION AND TESTING OF ANTIGEN-BINDING LYMPHOCYTES IN DIAGNOSTICS OF BRUCELLOSIS

Abstract. The aim of this study was to compare the efficiency of different methods applied for the diagnostics of brucellosis. Huddlson and Wright reactions, RoseBengal test, ELISA-based test system (IgG determination), and a test for antigen-binding lymphocytes (ABL) were used. There was shown that...

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Autores principales:	B. V. Karalnik, T. G. Denisova, G. B. Zhunusova, S. A. Fedosov, A. A. Zhankin, K. S. Ospanov, S. U. Mizanbaieva
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2014
Materias:	brucellosis diagnostics antibody antigen-binding lymphocytes Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/697da536780142cd89e64fbe33787d76
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spelling	oai:doaj.org-article:697da536780142cd89e64fbe33787d762021-11-18T08:03:42ZEFFICIENCY OF DIFFERENT TECHNIQUES FOR ANTIBODY DETERMINATION AND TESTING OF ANTIGEN-BINDING LYMPHOCYTES IN DIAGNOSTICS OF BRUCELLOSIS1563-06252313-741X10.15789/1563-0625-2006-4-567-572https://doaj.org/article/697da536780142cd89e64fbe33787d762014-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/504https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XAbstract. The aim of this study was to compare the efficiency of different methods applied for the diagnostics of brucellosis. Huddlson and Wright reactions, RoseBengal test, ELISA-based test system (IgG determination), and a test for antigen-binding lymphocytes (ABL) were used. There was shown that the specificity of tests was identical for either reactions based on detection of antibodies with commercial immune reagents and ELISA IgG. The sensitivity of ABL test is, however, superior to all the reactions based on antibody determination. The ABL test has advantages when compared with antibody detection techniques. This method permits early monitoring of brucellosis treatment/its efficiency since early terms, as well as differentiation between positive and false positive results of antibody determination in pregnant womеn.B. V. KaralnikT. G. DenisovaG. B. ZhunusovaS. A. FedosovA. A. ZhankinK. S. OspanovS. U. MizanbaievaSPb RAACIarticlebrucellosisdiagnosticsantibodyantigen-binding lymphocytesImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 8, Iss 4, Pp 567-572 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	brucellosis diagnostics antibody antigen-binding lymphocytes Immunologic diseases. Allergy RC581-607
spellingShingle	brucellosis diagnostics antibody antigen-binding lymphocytes Immunologic diseases. Allergy RC581-607 B. V. Karalnik T. G. Denisova G. B. Zhunusova S. A. Fedosov A. A. Zhankin K. S. Ospanov S. U. Mizanbaieva EFFICIENCY OF DIFFERENT TECHNIQUES FOR ANTIBODY DETERMINATION AND TESTING OF ANTIGEN-BINDING LYMPHOCYTES IN DIAGNOSTICS OF BRUCELLOSIS
description	Abstract. The aim of this study was to compare the efficiency of different methods applied for the diagnostics of brucellosis. Huddlson and Wright reactions, RoseBengal test, ELISA-based test system (IgG determination), and a test for antigen-binding lymphocytes (ABL) were used. There was shown that the specificity of tests was identical for either reactions based on detection of antibodies with commercial immune reagents and ELISA IgG. The sensitivity of ABL test is, however, superior to all the reactions based on antibody determination. The ABL test has advantages when compared with antibody detection techniques. This method permits early monitoring of brucellosis treatment/its efficiency since early terms, as well as differentiation between positive and false positive results of antibody determination in pregnant womеn.
format	article
author	B. V. Karalnik T. G. Denisova G. B. Zhunusova S. A. Fedosov A. A. Zhankin K. S. Ospanov S. U. Mizanbaieva
author_facet	B. V. Karalnik T. G. Denisova G. B. Zhunusova S. A. Fedosov A. A. Zhankin K. S. Ospanov S. U. Mizanbaieva
author_sort	B. V. Karalnik
title	EFFICIENCY OF DIFFERENT TECHNIQUES FOR ANTIBODY DETERMINATION AND TESTING OF ANTIGEN-BINDING LYMPHOCYTES IN DIAGNOSTICS OF BRUCELLOSIS
title_short	EFFICIENCY OF DIFFERENT TECHNIQUES FOR ANTIBODY DETERMINATION AND TESTING OF ANTIGEN-BINDING LYMPHOCYTES IN DIAGNOSTICS OF BRUCELLOSIS
title_full	EFFICIENCY OF DIFFERENT TECHNIQUES FOR ANTIBODY DETERMINATION AND TESTING OF ANTIGEN-BINDING LYMPHOCYTES IN DIAGNOSTICS OF BRUCELLOSIS
title_fullStr	EFFICIENCY OF DIFFERENT TECHNIQUES FOR ANTIBODY DETERMINATION AND TESTING OF ANTIGEN-BINDING LYMPHOCYTES IN DIAGNOSTICS OF BRUCELLOSIS
title_full_unstemmed	EFFICIENCY OF DIFFERENT TECHNIQUES FOR ANTIBODY DETERMINATION AND TESTING OF ANTIGEN-BINDING LYMPHOCYTES IN DIAGNOSTICS OF BRUCELLOSIS
title_sort	efficiency of different techniques for antibody determination and testing of antigen-binding lymphocytes in diagnostics of brucellosis
publisher	SPb RAACI
publishDate	2014
url	https://doaj.org/article/697da536780142cd89e64fbe33787d76
work_keys_str_mv	AT bvkaralnik efficiencyofdifferenttechniquesforantibodydeterminationandtestingofantigenbindinglymphocytesindiagnosticsofbrucellosis AT tgdenisova efficiencyofdifferenttechniquesforantibodydeterminationandtestingofantigenbindinglymphocytesindiagnosticsofbrucellosis AT gbzhunusova efficiencyofdifferenttechniquesforantibodydeterminationandtestingofantigenbindinglymphocytesindiagnosticsofbrucellosis AT safedosov efficiencyofdifferenttechniquesforantibodydeterminationandtestingofantigenbindinglymphocytesindiagnosticsofbrucellosis AT aazhankin efficiencyofdifferenttechniquesforantibodydeterminationandtestingofantigenbindinglymphocytesindiagnosticsofbrucellosis AT ksospanov efficiencyofdifferenttechniquesforantibodydeterminationandtestingofantigenbindinglymphocytesindiagnosticsofbrucellosis AT sumizanbaieva efficiencyofdifferenttechniquesforantibodydeterminationandtestingofantigenbindinglymphocytesindiagnosticsofbrucellosis
_version_	1718422482175655936

EFFICIENCY OF DIFFERENT TECHNIQUES FOR ANTIBODY DETERMINATION AND TESTING OF ANTIGEN-BINDING LYMPHOCYTES IN DIAGNOSTICS OF BRUCELLOSIS

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