Direct detection of polioviruses using a recombinant poliovirus receptor.

Polioviruses are positive-sense, single-stranded RNA picornaviruses and the principal cause of poliomyelitis. Global poliovirus surveillance has relied on poliovirus isolation in cells, which may take a minimum of 10 days, involves maintaining two cell lines, and propagates virus in high titers. Wit...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Nancy Gerloff, Mark Mandelbaum, Hong Pang, Nikail Collins, Brittani Brown, Hong Sun, Chelsea Harrington, Jessica Hecker, Chadi Agha, Cara C Burns, Everardo Vega
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6983332c4a8c4d09990888b86d1a7aef
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:6983332c4a8c4d09990888b86d1a7aef
record_format	dspace
spelling	oai:doaj.org-article:6983332c4a8c4d09990888b86d1a7aef2021-12-02T20:04:34ZDirect detection of polioviruses using a recombinant poliovirus receptor.1932-620310.1371/journal.pone.0259099https://doaj.org/article/6983332c4a8c4d09990888b86d1a7aef2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0259099https://doaj.org/toc/1932-6203Polioviruses are positive-sense, single-stranded RNA picornaviruses and the principal cause of poliomyelitis. Global poliovirus surveillance has relied on poliovirus isolation in cells, which may take a minimum of 10 days, involves maintaining two cell lines, and propagates virus in high titers. With eradication underway, a major objective of the Global Polio Eradication Initiative (GPEI) is to develop culture-independent detection of polioviruses as an alternative method to complement the current virus isolation technique. A culture-independent method on poliovirus-positive stool suspensions was assessed with commercially available recombinant soluble poliovirus receptor (PVR) coupled to Histidine (His) tags. Viral RNA was screened by quantitative real-time reverse transcription PCR using the poliovirus intratypic differentiation kit. Poliovirus recovery was optimized with PVR-His-tagged protein and buffers supplemented with polyethylene glycol. To validate the poliovirus-PVR-His tag purification assay, 182 poliovirus-positive stools of programmatic importance were parallel tested against the GPLN-accepted virus isolation method. The PVR-His tag enrichment method detected poliovirus in 164 of 171 poliovirus-positive stools, whereas the virus isolation method misidentified 38 stools as poliovirus-negative (McNemar χ2 p<0.0001). Using this method in combination with RNA extraction, viral RNA recovery increased and showed similar (WPV1) or higher (Sabin 1) sensitivity than the World Health Organization accredited variation of the virus isolation method. The PVR-His enrichment method could be a viable addition to poliovirus surveillance; similar methods have the potential to capture other human pathogens such as EV71 using an appropriate soluble His tag receptor.Nancy GerloffMark MandelbaumHong PangNikail CollinsBrittani BrownHong SunChelsea HarringtonJessica HeckerChadi AghaCara C BurnsEverardo VegaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 11, p e0259099 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nancy Gerloff Mark Mandelbaum Hong Pang Nikail Collins Brittani Brown Hong Sun Chelsea Harrington Jessica Hecker Chadi Agha Cara C Burns Everardo Vega Direct detection of polioviruses using a recombinant poliovirus receptor.
description	Polioviruses are positive-sense, single-stranded RNA picornaviruses and the principal cause of poliomyelitis. Global poliovirus surveillance has relied on poliovirus isolation in cells, which may take a minimum of 10 days, involves maintaining two cell lines, and propagates virus in high titers. With eradication underway, a major objective of the Global Polio Eradication Initiative (GPEI) is to develop culture-independent detection of polioviruses as an alternative method to complement the current virus isolation technique. A culture-independent method on poliovirus-positive stool suspensions was assessed with commercially available recombinant soluble poliovirus receptor (PVR) coupled to Histidine (His) tags. Viral RNA was screened by quantitative real-time reverse transcription PCR using the poliovirus intratypic differentiation kit. Poliovirus recovery was optimized with PVR-His-tagged protein and buffers supplemented with polyethylene glycol. To validate the poliovirus-PVR-His tag purification assay, 182 poliovirus-positive stools of programmatic importance were parallel tested against the GPLN-accepted virus isolation method. The PVR-His tag enrichment method detected poliovirus in 164 of 171 poliovirus-positive stools, whereas the virus isolation method misidentified 38 stools as poliovirus-negative (McNemar χ2 p<0.0001). Using this method in combination with RNA extraction, viral RNA recovery increased and showed similar (WPV1) or higher (Sabin 1) sensitivity than the World Health Organization accredited variation of the virus isolation method. The PVR-His enrichment method could be a viable addition to poliovirus surveillance; similar methods have the potential to capture other human pathogens such as EV71 using an appropriate soluble His tag receptor.
format	article
author	Nancy Gerloff Mark Mandelbaum Hong Pang Nikail Collins Brittani Brown Hong Sun Chelsea Harrington Jessica Hecker Chadi Agha Cara C Burns Everardo Vega
author_facet	Nancy Gerloff Mark Mandelbaum Hong Pang Nikail Collins Brittani Brown Hong Sun Chelsea Harrington Jessica Hecker Chadi Agha Cara C Burns Everardo Vega
author_sort	Nancy Gerloff
title	Direct detection of polioviruses using a recombinant poliovirus receptor.
title_short	Direct detection of polioviruses using a recombinant poliovirus receptor.
title_full	Direct detection of polioviruses using a recombinant poliovirus receptor.
title_fullStr	Direct detection of polioviruses using a recombinant poliovirus receptor.
title_full_unstemmed	Direct detection of polioviruses using a recombinant poliovirus receptor.
title_sort	direct detection of polioviruses using a recombinant poliovirus receptor.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/6983332c4a8c4d09990888b86d1a7aef
work_keys_str_mv	AT nancygerloff directdetectionofpoliovirusesusingarecombinantpoliovirusreceptor AT markmandelbaum directdetectionofpoliovirusesusingarecombinantpoliovirusreceptor AT hongpang directdetectionofpoliovirusesusingarecombinantpoliovirusreceptor AT nikailcollins directdetectionofpoliovirusesusingarecombinantpoliovirusreceptor AT brittanibrown directdetectionofpoliovirusesusingarecombinantpoliovirusreceptor AT hongsun directdetectionofpoliovirusesusingarecombinantpoliovirusreceptor AT chelseaharrington directdetectionofpoliovirusesusingarecombinantpoliovirusreceptor AT jessicahecker directdetectionofpoliovirusesusingarecombinantpoliovirusreceptor AT chadiagha directdetectionofpoliovirusesusingarecombinantpoliovirusreceptor AT caracburns directdetectionofpoliovirusesusingarecombinantpoliovirusreceptor AT everardovega directdetectionofpoliovirusesusingarecombinantpoliovirusreceptor
_version_	1718375546832814080

Direct detection of polioviruses using a recombinant poliovirus receptor.

Ejemplares similares