Salmonella chitosan nanoparticle vaccine administration is protective against Salmonella Enteritidis in broiler birds

Salmonella control strategies include vaccines that help reduce the spread of Salmonella in poultry flocks. In this study we evaluated the efficacy of administering a live Salmonella vaccine followed by a killed Salmonella chitosan nanoparticle (CNP) vaccine booster on the cellular and humoral immun...

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Autores principales:	Keila Y. Acevedo-Villanueva, Sankar Renu, Revathi Shanmugasundaram, Gabriel O. Akerele, Renukaradhy J. Gourapura, Ramesh K. Selvaraj
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6988e9ca72da424f9b1db69b80fafb37
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spelling	oai:doaj.org-article:6988e9ca72da424f9b1db69b80fafb372021-11-25T06:13:56ZSalmonella chitosan nanoparticle vaccine administration is protective against Salmonella Enteritidis in broiler birds1932-6203https://doaj.org/article/6988e9ca72da424f9b1db69b80fafb372021-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8594846/?tool=EBIhttps://doaj.org/toc/1932-6203Salmonella control strategies include vaccines that help reduce the spread of Salmonella in poultry flocks. In this study we evaluated the efficacy of administering a live Salmonella vaccine followed by a killed Salmonella chitosan nanoparticle (CNP) vaccine booster on the cellular and humoral immunity of broilers. The CNP vaccine was synthesized with Salmonella Enteritidis (S. Enteritidis) outer-membrane-proteins (OMPs) and flagellin-proteins. At d1-of-age, one-hundred-sixty-eight chicks were allocated into treatments: 1) No vaccine, 2) Live vaccine (Poulvac®ST), 3) CNP vaccine, 4) Live+CNP vaccine. At d1-of-age, birds were orally vaccinated with PBS, Live vaccine, or CNP. At d7-of-age, the No vaccine, Live vaccine and CNP vaccine groups were boosted with PBS and the Live+CNP vaccine group was boosted with CNP. At d14-of-age, birds were challenged with 1×109 CFU/bird S. Enteritidis. There were no significant differences in body-weight-gain (BWG) or feed-conversion-ratio (FCR). At 8h-post-challenge, CNP and Live+CNP-vaccinated birds had 17% and 24% greater levels (P<0.05) of anti-Salmonella OMPs IgA in bile, respectively, compared to control. At d28-of-age, CNP, Live, and Live+CNP-vaccinated birds had 33%, 18%, and 24% greater levels (P<0.05) of anti-Salmonella OMPs IgA in bile, respectively, compared to control. At d14-of-age, Live+CNP-vaccinated birds had 46% greater levels (P<0.05) of anti-Salmonella OMPs IgY in serum, compared to control. At d21-of-age, splenocytes from CNP and Live-vaccinated birds had increased (P<0.05) T-lymphocyte proliferation at 0.02 mg/mL OMPs stimulation compared to the control. At d28-of-age, CNP and Live+CNP-vaccinated birds had 0.9 Log10 CFU/g and 1 Log10 CFU/g decreased S. Enteritidis cecal loads (P<0.05), respectively, compared to control. The CNP vaccine does not have adverse effects on bird’s BWG and FCR or IL-1β, IL-10, IFN-γ, or iNOS mRNA expression levels. It can be concluded that the CNP vaccine, as a first dose or as a booster vaccination, is an alternative vaccine candidate against S. Enteritidis in broilers.Keila Y. Acevedo-VillanuevaSankar RenuRevathi ShanmugasundaramGabriel O. AkereleRenukaradhy J. GourapuraRamesh K. SelvarajPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Keila Y. Acevedo-Villanueva Sankar Renu Revathi Shanmugasundaram Gabriel O. Akerele Renukaradhy J. Gourapura Ramesh K. Selvaraj Salmonella chitosan nanoparticle vaccine administration is protective against Salmonella Enteritidis in broiler birds
description	Salmonella control strategies include vaccines that help reduce the spread of Salmonella in poultry flocks. In this study we evaluated the efficacy of administering a live Salmonella vaccine followed by a killed Salmonella chitosan nanoparticle (CNP) vaccine booster on the cellular and humoral immunity of broilers. The CNP vaccine was synthesized with Salmonella Enteritidis (S. Enteritidis) outer-membrane-proteins (OMPs) and flagellin-proteins. At d1-of-age, one-hundred-sixty-eight chicks were allocated into treatments: 1) No vaccine, 2) Live vaccine (Poulvac®ST), 3) CNP vaccine, 4) Live+CNP vaccine. At d1-of-age, birds were orally vaccinated with PBS, Live vaccine, or CNP. At d7-of-age, the No vaccine, Live vaccine and CNP vaccine groups were boosted with PBS and the Live+CNP vaccine group was boosted with CNP. At d14-of-age, birds were challenged with 1×109 CFU/bird S. Enteritidis. There were no significant differences in body-weight-gain (BWG) or feed-conversion-ratio (FCR). At 8h-post-challenge, CNP and Live+CNP-vaccinated birds had 17% and 24% greater levels (P<0.05) of anti-Salmonella OMPs IgA in bile, respectively, compared to control. At d28-of-age, CNP, Live, and Live+CNP-vaccinated birds had 33%, 18%, and 24% greater levels (P<0.05) of anti-Salmonella OMPs IgA in bile, respectively, compared to control. At d14-of-age, Live+CNP-vaccinated birds had 46% greater levels (P<0.05) of anti-Salmonella OMPs IgY in serum, compared to control. At d21-of-age, splenocytes from CNP and Live-vaccinated birds had increased (P<0.05) T-lymphocyte proliferation at 0.02 mg/mL OMPs stimulation compared to the control. At d28-of-age, CNP and Live+CNP-vaccinated birds had 0.9 Log10 CFU/g and 1 Log10 CFU/g decreased S. Enteritidis cecal loads (P<0.05), respectively, compared to control. The CNP vaccine does not have adverse effects on bird’s BWG and FCR or IL-1β, IL-10, IFN-γ, or iNOS mRNA expression levels. It can be concluded that the CNP vaccine, as a first dose or as a booster vaccination, is an alternative vaccine candidate against S. Enteritidis in broilers.
format	article
author	Keila Y. Acevedo-Villanueva Sankar Renu Revathi Shanmugasundaram Gabriel O. Akerele Renukaradhy J. Gourapura Ramesh K. Selvaraj
author_facet	Keila Y. Acevedo-Villanueva Sankar Renu Revathi Shanmugasundaram Gabriel O. Akerele Renukaradhy J. Gourapura Ramesh K. Selvaraj
author_sort	Keila Y. Acevedo-Villanueva
title	Salmonella chitosan nanoparticle vaccine administration is protective against Salmonella Enteritidis in broiler birds
title_short	Salmonella chitosan nanoparticle vaccine administration is protective against Salmonella Enteritidis in broiler birds
title_full	Salmonella chitosan nanoparticle vaccine administration is protective against Salmonella Enteritidis in broiler birds
title_fullStr	Salmonella chitosan nanoparticle vaccine administration is protective against Salmonella Enteritidis in broiler birds
title_full_unstemmed	Salmonella chitosan nanoparticle vaccine administration is protective against Salmonella Enteritidis in broiler birds
title_sort	salmonella chitosan nanoparticle vaccine administration is protective against salmonella enteritidis in broiler birds
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/6988e9ca72da424f9b1db69b80fafb37
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Salmonella chitosan nanoparticle vaccine administration is protective against Salmonella Enteritidis in broiler birds

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