Disruption of the plant-specific CFS1 gene impairs autophagosome turnover and triggers EDS1-dependent cell death
Abstract Cell death, autophagy and endosomal sorting contribute to many physiological, developmental and immunological processes in plants. They are mechanistically interconnected and interdependent, but the molecular basis of their mutual regulation has only begun to emerge in plants. Here, we desc...
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Nature Portfolio
2017
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oai:doaj.org-article:6992c0ebf240486fa7c47bdd6f4fefa62021-12-02T16:08:09ZDisruption of the plant-specific CFS1 gene impairs autophagosome turnover and triggers EDS1-dependent cell death10.1038/s41598-017-08577-82045-2322https://doaj.org/article/6992c0ebf240486fa7c47bdd6f4fefa62017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08577-8https://doaj.org/toc/2045-2322Abstract Cell death, autophagy and endosomal sorting contribute to many physiological, developmental and immunological processes in plants. They are mechanistically interconnected and interdependent, but the molecular basis of their mutual regulation has only begun to emerge in plants. Here, we describe the identification and molecular characterization of CELL DEATH RELATED ENDOSOMAL FYVE/SYLF PROTEIN 1 (CFS1). The CFS1 protein interacts with the ENDOSOMAL SORTING COMPLEX REQUIRED FOR TRANSPORT I (ESCRT-I) component ELCH (ELC) and is localized at ESCRT-I-positive late endosomes likely through its PI3P and actin binding SH3YL1 Ysc84/Lsb4p Lsb3p plant FYVE (SYLF) domain. Mutant alleles of cfs1 exhibit auto-immune phenotypes including spontaneous lesions that show characteristics of hypersensitive response (HR). Autoimmunity in cfs1 is dependent on ENHANCED DISEASE SUSCEPTIBILITY 1 (EDS1)-mediated effector-triggered immunity (ETI) but independent from salicylic acid. Additionally, cfs1 mutants accumulate the autophagy markers ATG8 and NBR1 independently from EDS1. We hypothesize that CFS1 acts at the intersection of autophagosomes and endosomes and contributes to cellular homeostasis by mediating autophagosome turnover.Arpaporn SutipatanasomboonStefanie HerberthEllen G. AlwoodHeidrun HäwekerBritta MüllerMojgan ShahriariAnke Y. ZienertBirger MarinSilke RobatzekGerrit J. K. PraefckeKathryn R. AyscoughMartin HülskampSwen SchellmannNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017) |
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Medicine R Science Q Arpaporn Sutipatanasomboon Stefanie Herberth Ellen G. Alwood Heidrun Häweker Britta Müller Mojgan Shahriari Anke Y. Zienert Birger Marin Silke Robatzek Gerrit J. K. Praefcke Kathryn R. Ayscough Martin Hülskamp Swen Schellmann Disruption of the plant-specific CFS1 gene impairs autophagosome turnover and triggers EDS1-dependent cell death |
| description |
Abstract Cell death, autophagy and endosomal sorting contribute to many physiological, developmental and immunological processes in plants. They are mechanistically interconnected and interdependent, but the molecular basis of their mutual regulation has only begun to emerge in plants. Here, we describe the identification and molecular characterization of CELL DEATH RELATED ENDOSOMAL FYVE/SYLF PROTEIN 1 (CFS1). The CFS1 protein interacts with the ENDOSOMAL SORTING COMPLEX REQUIRED FOR TRANSPORT I (ESCRT-I) component ELCH (ELC) and is localized at ESCRT-I-positive late endosomes likely through its PI3P and actin binding SH3YL1 Ysc84/Lsb4p Lsb3p plant FYVE (SYLF) domain. Mutant alleles of cfs1 exhibit auto-immune phenotypes including spontaneous lesions that show characteristics of hypersensitive response (HR). Autoimmunity in cfs1 is dependent on ENHANCED DISEASE SUSCEPTIBILITY 1 (EDS1)-mediated effector-triggered immunity (ETI) but independent from salicylic acid. Additionally, cfs1 mutants accumulate the autophagy markers ATG8 and NBR1 independently from EDS1. We hypothesize that CFS1 acts at the intersection of autophagosomes and endosomes and contributes to cellular homeostasis by mediating autophagosome turnover. |
| format |
article |
| author |
Arpaporn Sutipatanasomboon Stefanie Herberth Ellen G. Alwood Heidrun Häweker Britta Müller Mojgan Shahriari Anke Y. Zienert Birger Marin Silke Robatzek Gerrit J. K. Praefcke Kathryn R. Ayscough Martin Hülskamp Swen Schellmann |
| author_facet |
Arpaporn Sutipatanasomboon Stefanie Herberth Ellen G. Alwood Heidrun Häweker Britta Müller Mojgan Shahriari Anke Y. Zienert Birger Marin Silke Robatzek Gerrit J. K. Praefcke Kathryn R. Ayscough Martin Hülskamp Swen Schellmann |
| author_sort |
Arpaporn Sutipatanasomboon |
| title |
Disruption of the plant-specific CFS1 gene impairs autophagosome turnover and triggers EDS1-dependent cell death |
| title_short |
Disruption of the plant-specific CFS1 gene impairs autophagosome turnover and triggers EDS1-dependent cell death |
| title_full |
Disruption of the plant-specific CFS1 gene impairs autophagosome turnover and triggers EDS1-dependent cell death |
| title_fullStr |
Disruption of the plant-specific CFS1 gene impairs autophagosome turnover and triggers EDS1-dependent cell death |
| title_full_unstemmed |
Disruption of the plant-specific CFS1 gene impairs autophagosome turnover and triggers EDS1-dependent cell death |
| title_sort |
disruption of the plant-specific cfs1 gene impairs autophagosome turnover and triggers eds1-dependent cell death |
| publisher |
Nature Portfolio |
| publishDate |
2017 |
| url |
https://doaj.org/article/6992c0ebf240486fa7c47bdd6f4fefa6 |
| work_keys_str_mv |
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