Molecular epidemiology of nontypeable Haemophilus influenzae causing community-acquired pneumonia in adults.

Nontypeable Haemophilus influenzae (NTHi) is an opportunistic pathogen which causes a variety of respiratory infections. The objectives of the study were to determine its antimicrobial susceptibility, to characterize the β-lactam resistance, and to establish a genetic characterization of NTHi isolat...

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Autores principales:	Carmen Puig, Laura Calatayud, Sara Martí, Fe Tubau, Carolina Garcia-Vidal, Jordi Carratalà, Josefina Liñares, Carmen Ardanuy
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/699f3a689dd8445caf9c44ee94432141
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spelling	oai:doaj.org-article:699f3a689dd8445caf9c44ee944321412021-11-18T08:42:01ZMolecular epidemiology of nontypeable Haemophilus influenzae causing community-acquired pneumonia in adults.1932-620310.1371/journal.pone.0082515https://doaj.org/article/699f3a689dd8445caf9c44ee944321412013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24349303/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Nontypeable Haemophilus influenzae (NTHi) is an opportunistic pathogen which causes a variety of respiratory infections. The objectives of the study were to determine its antimicrobial susceptibility, to characterize the β-lactam resistance, and to establish a genetic characterization of NTHi isolates. Ninety-five NTHi isolates were analyzed by pulsed field gel electrophoresis (PFGE) and multi locus sequence typing (MLST). Antimicrobial susceptibility was determined by microdilution, and the ftsI gene (encoding penicillin-binding protein 3, PBP3) was PCR amplified and sequenced. Thirty (31.6%) isolates were non-susceptible to ampicillin (MIC ≥ 2 mg/L), with 10 of them producing β-lactamase type TEM-1 as a resistance mechanism. After ftsI sequencing, 39 (41.1%) isolates showed amino acid substitutions in PBP3, with Asn526 → Lys being the most common (69.2%). Eighty-four patients were successfully treated with amoxicillin/clavulanic acid, ceftriaxone and levofloxacin. Eight patients died due either to aspiration or complication of their comorbidities. In conclusion, NTHi causing CAP in adults shows high genetic diversity and is associated with a high rate of reduced susceptibility to ampicillin due to alterations in PBP3. The analysis of treatment and outcomes demonstrated that NTHi strains with mutations in the ftsI gene could be successfully treated with ceftriaxone or fluoroquinolones.Carmen PuigLaura CalatayudSara MartíFe TubauCarolina Garcia-VidalJordi CarratalàJosefina LiñaresCarmen ArdanuyPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 12, p e82515 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Carmen Puig Laura Calatayud Sara Martí Fe Tubau Carolina Garcia-Vidal Jordi Carratalà Josefina Liñares Carmen Ardanuy Molecular epidemiology of nontypeable Haemophilus influenzae causing community-acquired pneumonia in adults.
description	Nontypeable Haemophilus influenzae (NTHi) is an opportunistic pathogen which causes a variety of respiratory infections. The objectives of the study were to determine its antimicrobial susceptibility, to characterize the β-lactam resistance, and to establish a genetic characterization of NTHi isolates. Ninety-five NTHi isolates were analyzed by pulsed field gel electrophoresis (PFGE) and multi locus sequence typing (MLST). Antimicrobial susceptibility was determined by microdilution, and the ftsI gene (encoding penicillin-binding protein 3, PBP3) was PCR amplified and sequenced. Thirty (31.6%) isolates were non-susceptible to ampicillin (MIC ≥ 2 mg/L), with 10 of them producing β-lactamase type TEM-1 as a resistance mechanism. After ftsI sequencing, 39 (41.1%) isolates showed amino acid substitutions in PBP3, with Asn526 → Lys being the most common (69.2%). Eighty-four patients were successfully treated with amoxicillin/clavulanic acid, ceftriaxone and levofloxacin. Eight patients died due either to aspiration or complication of their comorbidities. In conclusion, NTHi causing CAP in adults shows high genetic diversity and is associated with a high rate of reduced susceptibility to ampicillin due to alterations in PBP3. The analysis of treatment and outcomes demonstrated that NTHi strains with mutations in the ftsI gene could be successfully treated with ceftriaxone or fluoroquinolones.
format	article
author	Carmen Puig Laura Calatayud Sara Martí Fe Tubau Carolina Garcia-Vidal Jordi Carratalà Josefina Liñares Carmen Ardanuy
author_facet	Carmen Puig Laura Calatayud Sara Martí Fe Tubau Carolina Garcia-Vidal Jordi Carratalà Josefina Liñares Carmen Ardanuy
author_sort	Carmen Puig
title	Molecular epidemiology of nontypeable Haemophilus influenzae causing community-acquired pneumonia in adults.
title_short	Molecular epidemiology of nontypeable Haemophilus influenzae causing community-acquired pneumonia in adults.
title_full	Molecular epidemiology of nontypeable Haemophilus influenzae causing community-acquired pneumonia in adults.
title_fullStr	Molecular epidemiology of nontypeable Haemophilus influenzae causing community-acquired pneumonia in adults.
title_full_unstemmed	Molecular epidemiology of nontypeable Haemophilus influenzae causing community-acquired pneumonia in adults.
title_sort	molecular epidemiology of nontypeable haemophilus influenzae causing community-acquired pneumonia in adults.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/699f3a689dd8445caf9c44ee94432141
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Molecular epidemiology of nontypeable Haemophilus influenzae causing community-acquired pneumonia in adults.

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