Novel multi-biotin grafted poly(lactic acid) and its self-assembling nanoparticles capable of binding to streptavidin

Hao Yan1,2, Weimin Jiang1,2, Yinxing Zhang1,2, Ying Liu1,2, Bin Wang1,2, Li Yang1,2, Lihong Deng1,2, Gurinder K Singh1,2, Jun Pan1,21Bioengineering College, Chongqing University, 2Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Chongqing, People...

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Autores principales: Yan H, Jiang W, Zhang Y, Liu Y, Wang B, Yang L, Deng L, Singh GK, Pan J
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Lenguaje:EN
Publicado: Dove Medical Press 2012
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spelling oai:doaj.org-article:69ab6bdb57f5495cbc677cb37dc221cb2021-12-02T00:04:48ZNovel multi-biotin grafted poly(lactic acid) and its self-assembling nanoparticles capable of binding to streptavidin1176-91141178-2013https://doaj.org/article/69ab6bdb57f5495cbc677cb37dc221cb2012-01-01T00:00:00Zhttp://www.dovepress.com/novel-multi-biotin-grafted-polylactic-acid-and-its-self-assembling-nan-a9153https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Hao Yan1,2, Weimin Jiang1,2, Yinxing Zhang1,2, Ying Liu1,2, Bin Wang1,2, Li Yang1,2, Lihong Deng1,2, Gurinder K Singh1,2, Jun Pan1,21Bioengineering College, Chongqing University, 2Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Chongqing, People's Republic of ChinaAbstract: Targeted drug delivery requires novel biodegradable, specific binding systems with longer circulation time. The aim of this study was to prepare biotinylated poly(lactic acid) (PLA) nanoparticles (NPs) which can meet regular requirements as well conjugate more biotins in the polymer to provide better binding with streptavidin. A biotin-graft-PLA was synthesized based on previously published biodegradable poly(ethylene glycol) (PEG)-graft-PLA, with one polymer molecule containing three PEG molecules. Newly synthesized biotin-graft-PLA had three biotins per polymer molecule, higher than the previous biotinylated PLA (≤1 biotin per polymer molecule). A PEG with a much lower molecular weight (MW ~1900) than the previous biotinylated PLA (PEG MW ≥3800), and thus more biocompatible, was used which supplied good nonspecific protein-resistant property compatible to PEG-graft-PLA, suggesting its possible longer stay in the bloodstream. Biotin-graft-PLA specifically bound to streptavidin and self-assembled into NPs, during which naproxen, a model small molecule (MW 230 Da) and hydrophobic drug, was encapsulated (encapsulation efficiency 51.88%). The naproxen-loaded NPs with particle size and zeta potential of 175 nm and —27.35 mV realized controlled release within 170 hours, comparable to previous studies. The biotin-graft-PLA NPs adhered approximately two-fold more on streptavidin film and on biotin film via a streptavidin arm both in static and dynamic conditions compared with PEG-graft-PLA NPs, the proven nonspecific protein-resistant NPs. The specific binding of biotin-graft-PLA NPs with streptavidin and with biotin using streptavidin arm, as well as its entrapment and controlled release for naproxen, suggest potential applications in targeted drug delivery.Keywords: targeted drug delivery, bioactive, biodegradable, poly(ethylene glycol) (PEG), controlled release, naproxenYan HJiang WZhang YLiu YWang BYang LDeng LSingh GKPan JDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 457-465 (2012)
institution DOAJ
collection DOAJ
language EN
topic Medicine (General)
R5-920
spellingShingle Medicine (General)
R5-920
Yan H
Jiang W
Zhang Y
Liu Y
Wang B
Yang L
Deng L
Singh GK
Pan J
Novel multi-biotin grafted poly(lactic acid) and its self-assembling nanoparticles capable of binding to streptavidin
description Hao Yan1,2, Weimin Jiang1,2, Yinxing Zhang1,2, Ying Liu1,2, Bin Wang1,2, Li Yang1,2, Lihong Deng1,2, Gurinder K Singh1,2, Jun Pan1,21Bioengineering College, Chongqing University, 2Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Chongqing, People's Republic of ChinaAbstract: Targeted drug delivery requires novel biodegradable, specific binding systems with longer circulation time. The aim of this study was to prepare biotinylated poly(lactic acid) (PLA) nanoparticles (NPs) which can meet regular requirements as well conjugate more biotins in the polymer to provide better binding with streptavidin. A biotin-graft-PLA was synthesized based on previously published biodegradable poly(ethylene glycol) (PEG)-graft-PLA, with one polymer molecule containing three PEG molecules. Newly synthesized biotin-graft-PLA had three biotins per polymer molecule, higher than the previous biotinylated PLA (≤1 biotin per polymer molecule). A PEG with a much lower molecular weight (MW ~1900) than the previous biotinylated PLA (PEG MW ≥3800), and thus more biocompatible, was used which supplied good nonspecific protein-resistant property compatible to PEG-graft-PLA, suggesting its possible longer stay in the bloodstream. Biotin-graft-PLA specifically bound to streptavidin and self-assembled into NPs, during which naproxen, a model small molecule (MW 230 Da) and hydrophobic drug, was encapsulated (encapsulation efficiency 51.88%). The naproxen-loaded NPs with particle size and zeta potential of 175 nm and —27.35 mV realized controlled release within 170 hours, comparable to previous studies. The biotin-graft-PLA NPs adhered approximately two-fold more on streptavidin film and on biotin film via a streptavidin arm both in static and dynamic conditions compared with PEG-graft-PLA NPs, the proven nonspecific protein-resistant NPs. The specific binding of biotin-graft-PLA NPs with streptavidin and with biotin using streptavidin arm, as well as its entrapment and controlled release for naproxen, suggest potential applications in targeted drug delivery.Keywords: targeted drug delivery, bioactive, biodegradable, poly(ethylene glycol) (PEG), controlled release, naproxen
format article
author Yan H
Jiang W
Zhang Y
Liu Y
Wang B
Yang L
Deng L
Singh GK
Pan J
author_facet Yan H
Jiang W
Zhang Y
Liu Y
Wang B
Yang L
Deng L
Singh GK
Pan J
author_sort Yan H
title Novel multi-biotin grafted poly(lactic acid) and its self-assembling nanoparticles capable of binding to streptavidin
title_short Novel multi-biotin grafted poly(lactic acid) and its self-assembling nanoparticles capable of binding to streptavidin
title_full Novel multi-biotin grafted poly(lactic acid) and its self-assembling nanoparticles capable of binding to streptavidin
title_fullStr Novel multi-biotin grafted poly(lactic acid) and its self-assembling nanoparticles capable of binding to streptavidin
title_full_unstemmed Novel multi-biotin grafted poly(lactic acid) and its self-assembling nanoparticles capable of binding to streptavidin
title_sort novel multi-biotin grafted poly(lactic acid) and its self-assembling nanoparticles capable of binding to streptavidin
publisher Dove Medical Press
publishDate 2012
url https://doaj.org/article/69ab6bdb57f5495cbc677cb37dc221cb
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