Multiple scattering effects on intercept, size, polydispersity index, and intensity for parallel (VV) and perpendicular (VH) polarization detection in photon correlation spectroscopy

Abstract Dynamic light scattering (DLS) is well established for rapid size, polydispersity, and size distribution determination of colloidal samples. While there are limitations in size range, resolution, and concentration, the technique has found ubiquitous applications from molecules to particles....

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Autores principales:	Ragy Ragheb, Ulf Nobbmann
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/69ac858068cf474ab4679c99a4542c66
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spelling	oai:doaj.org-article:69ac858068cf474ab4679c99a4542c662021-12-02T15:11:50ZMultiple scattering effects on intercept, size, polydispersity index, and intensity for parallel (VV) and perpendicular (VH) polarization detection in photon correlation spectroscopy10.1038/s41598-020-78872-42045-2322https://doaj.org/article/69ac858068cf474ab4679c99a4542c662020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78872-4https://doaj.org/toc/2045-2322Abstract Dynamic light scattering (DLS) is well established for rapid size, polydispersity, and size distribution determination of colloidal samples. While there are limitations in size range, resolution, and concentration, the technique has found ubiquitous applications from molecules to particles. With the ease of use of today’s commercial DLS instrumentation comes an inherent danger of misinterpretation or misapplication at the borderlines of suitability. In this paper, we show how comparison of different polarization components can help ascertain the presence of unwanted multiple scattering, which can lead to false conclusions about a sample’s mean size and polydispersity. We find that the contribution of multiple scattering events effectively reduces both the measured scattering intensity and the apparent size from the autocorrelation function. The intercept of the correlation function may serve as an indicator of relative strength of single to multiple scattering. Furthermore, the abundance of single scattering events at measurement positions close to the cell wall results in an apparent increase in uniformity yielding a lower polydispersity index which is more representative of the physical system.Ragy RaghebUlf NobbmannNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-9 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ragy Ragheb Ulf Nobbmann Multiple scattering effects on intercept, size, polydispersity index, and intensity for parallel (VV) and perpendicular (VH) polarization detection in photon correlation spectroscopy
description	Abstract Dynamic light scattering (DLS) is well established for rapid size, polydispersity, and size distribution determination of colloidal samples. While there are limitations in size range, resolution, and concentration, the technique has found ubiquitous applications from molecules to particles. With the ease of use of today’s commercial DLS instrumentation comes an inherent danger of misinterpretation or misapplication at the borderlines of suitability. In this paper, we show how comparison of different polarization components can help ascertain the presence of unwanted multiple scattering, which can lead to false conclusions about a sample’s mean size and polydispersity. We find that the contribution of multiple scattering events effectively reduces both the measured scattering intensity and the apparent size from the autocorrelation function. The intercept of the correlation function may serve as an indicator of relative strength of single to multiple scattering. Furthermore, the abundance of single scattering events at measurement positions close to the cell wall results in an apparent increase in uniformity yielding a lower polydispersity index which is more representative of the physical system.
format	article
author	Ragy Ragheb Ulf Nobbmann
author_facet	Ragy Ragheb Ulf Nobbmann
author_sort	Ragy Ragheb
title	Multiple scattering effects on intercept, size, polydispersity index, and intensity for parallel (VV) and perpendicular (VH) polarization detection in photon correlation spectroscopy
title_short	Multiple scattering effects on intercept, size, polydispersity index, and intensity for parallel (VV) and perpendicular (VH) polarization detection in photon correlation spectroscopy
title_full	Multiple scattering effects on intercept, size, polydispersity index, and intensity for parallel (VV) and perpendicular (VH) polarization detection in photon correlation spectroscopy
title_fullStr	Multiple scattering effects on intercept, size, polydispersity index, and intensity for parallel (VV) and perpendicular (VH) polarization detection in photon correlation spectroscopy
title_full_unstemmed	Multiple scattering effects on intercept, size, polydispersity index, and intensity for parallel (VV) and perpendicular (VH) polarization detection in photon correlation spectroscopy
title_sort	multiple scattering effects on intercept, size, polydispersity index, and intensity for parallel (vv) and perpendicular (vh) polarization detection in photon correlation spectroscopy
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/69ac858068cf474ab4679c99a4542c66
work_keys_str_mv	AT ragyragheb multiplescatteringeffectsoninterceptsizepolydispersityindexandintensityforparallelvvandperpendicularvhpolarizationdetectioninphotoncorrelationspectroscopy AT ulfnobbmann multiplescatteringeffectsoninterceptsizepolydispersityindexandintensityforparallelvvandperpendicularvhpolarizationdetectioninphotoncorrelationspectroscopy
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Multiple scattering effects on intercept, size, polydispersity index, and intensity for parallel (VV) and perpendicular (VH) polarization detection in photon correlation spectroscopy

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