Effective Decontamination and Regeneration Protocol for in vitro Culture of Strawberry Cv. Chandler

Nodal segments of strawberry cv. Chandler were collected from the field and surface sterilization of the explants was carried out using various levels of decontaminants as different treatments viz. sodium hypochlorite (NaClO) 0.5% for 7, 5 and 3 min and mercuric chloride (Hg Cl2) 0.1% for 5 min, 3 m...

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Autores principales:	S Diengngan, B N S Murthy, M Mahadevamma
Formato:	article
Lenguaje:	EN
Publicado:	Society for Promotion of Horticulture - Indian Institute of Horticultural Research 2014
Materias:	decontaminants growth regulators in vitro nodal segments strawberry Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/69ae19f022674fba9aac8ac7106dbe5c
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spelling	oai:doaj.org-article:69ae19f022674fba9aac8ac7106dbe5c2021-12-02T10:22:29ZEffective Decontamination and Regeneration Protocol for in vitro Culture of Strawberry Cv. Chandler0973-354X2582-4899https://doaj.org/article/69ae19f022674fba9aac8ac7106dbe5c2014-12-01T00:00:00Zhttps://jhs.iihr.res.in/index.php/jhs/article/view/181https://doaj.org/toc/0973-354Xhttps://doaj.org/toc/2582-4899Nodal segments of strawberry cv. Chandler were collected from the field and surface sterilization of the explants was carried out using various levels of decontaminants as different treatments viz. sodium hypochlorite (NaClO) 0.5% for 7, 5 and 3 min and mercuric chloride (Hg Cl2) 0.1% for 5 min, 3 min and 3 min 10 sec. The explants were excised and cultured for 3 weeks on the initiation medium supplemented with various levels of BAP (0.5, 1, 1.5 and 2mg/l) and its combination with GA (0.5 and 1mg/l). Sub culturing was done and finally rooting was initiated on the medium supplemented with various levels of IBA (0.5, 1, 1.5 and 2mg/l) for 4 weeks. The results obtained indicated that, among the decontaminants, treatment of explants with Hg Cl2 0.1% for 3 min 10 sec resulted in the minimum contamination and browning of explants. Further, maximum shoot proliferation percentage, shoots per explant and minimum number of days to shoot initiation was observed when explants were cultured on MS medium supplemented with 1.5 mg/l BAP over other concentrations of either BAP or in combination with GA. However, the maximum length of shoots was obtained in medium supplemented with 1.5 mg/l BAP + 0.5 mg/l GA. While, medium supplemented with 0.5 mg/l IBA supported highest percentage of rooting, the highest number of roots, maximum root length of microcuttings and minimum number of days to root initiation were observed in medium supplemented with 1mg/l of IBA.S DiengnganB N S MurthyM MahadevammaSociety for Promotion of Horticulture - Indian Institute of Horticultural Researcharticledecontaminantsgrowth regulatorsin vitronodal segmentsstrawberryPlant cultureSB1-1110ENJournal of Horticultural Sciences, Vol 9, Iss 2, Pp 126-130 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	decontaminants growth regulators in vitro nodal segments strawberry Plant culture SB1-1110
spellingShingle	decontaminants growth regulators in vitro nodal segments strawberry Plant culture SB1-1110 S Diengngan B N S Murthy M Mahadevamma Effective Decontamination and Regeneration Protocol for in vitro Culture of Strawberry Cv. Chandler
description	Nodal segments of strawberry cv. Chandler were collected from the field and surface sterilization of the explants was carried out using various levels of decontaminants as different treatments viz. sodium hypochlorite (NaClO) 0.5% for 7, 5 and 3 min and mercuric chloride (Hg Cl2) 0.1% for 5 min, 3 min and 3 min 10 sec. The explants were excised and cultured for 3 weeks on the initiation medium supplemented with various levels of BAP (0.5, 1, 1.5 and 2mg/l) and its combination with GA (0.5 and 1mg/l). Sub culturing was done and finally rooting was initiated on the medium supplemented with various levels of IBA (0.5, 1, 1.5 and 2mg/l) for 4 weeks. The results obtained indicated that, among the decontaminants, treatment of explants with Hg Cl2 0.1% for 3 min 10 sec resulted in the minimum contamination and browning of explants. Further, maximum shoot proliferation percentage, shoots per explant and minimum number of days to shoot initiation was observed when explants were cultured on MS medium supplemented with 1.5 mg/l BAP over other concentrations of either BAP or in combination with GA. However, the maximum length of shoots was obtained in medium supplemented with 1.5 mg/l BAP + 0.5 mg/l GA. While, medium supplemented with 0.5 mg/l IBA supported highest percentage of rooting, the highest number of roots, maximum root length of microcuttings and minimum number of days to root initiation were observed in medium supplemented with 1mg/l of IBA.
format	article
author	S Diengngan B N S Murthy M Mahadevamma
author_facet	S Diengngan B N S Murthy M Mahadevamma
author_sort	S Diengngan
title	Effective Decontamination and Regeneration Protocol for in vitro Culture of Strawberry Cv. Chandler
title_short	Effective Decontamination and Regeneration Protocol for in vitro Culture of Strawberry Cv. Chandler
title_full	Effective Decontamination and Regeneration Protocol for in vitro Culture of Strawberry Cv. Chandler
title_fullStr	Effective Decontamination and Regeneration Protocol for in vitro Culture of Strawberry Cv. Chandler
title_full_unstemmed	Effective Decontamination and Regeneration Protocol for in vitro Culture of Strawberry Cv. Chandler
title_sort	effective decontamination and regeneration protocol for in vitro culture of strawberry cv. chandler
publisher	Society for Promotion of Horticulture - Indian Institute of Horticultural Research
publishDate	2014
url	https://doaj.org/article/69ae19f022674fba9aac8ac7106dbe5c
work_keys_str_mv	AT sdiengngan effectivedecontaminationandregenerationprotocolforinvitrocultureofstrawberrycvchandler AT bnsmurthy effectivedecontaminationandregenerationprotocolforinvitrocultureofstrawberrycvchandler AT mmahadevamma effectivedecontaminationandregenerationprotocolforinvitrocultureofstrawberrycvchandler
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Effective Decontamination and Regeneration Protocol for in vitro Culture of Strawberry Cv. Chandler

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