Antennal and abdominal transcriptomes reveal chemosensory gene families in the coconut hispine beetle, Brontispa longissima

Abstract Antennal and abdominal transcriptomes of males and females of the coconut hispine beetle Brontispa longissima were sequenced to identify and compare the expression patterns of genes involved in odorant reception and detection. Representative proteins from the chemosensory gene families like...

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Autores principales:	Shu-Ying Bin, Meng-Qiu Qu, Ke-Ming Li, Zheng-Qiang Peng, Zhong-Zhen Wu, Jin-Tian Lin
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/69b6ac78ace949e781843d9497779b58
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spelling	oai:doaj.org-article:69b6ac78ace949e781843d9497779b582021-12-02T12:32:37ZAntennal and abdominal transcriptomes reveal chemosensory gene families in the coconut hispine beetle, Brontispa longissima10.1038/s41598-017-03263-12045-2322https://doaj.org/article/69b6ac78ace949e781843d9497779b582017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03263-1https://doaj.org/toc/2045-2322Abstract Antennal and abdominal transcriptomes of males and females of the coconut hispine beetle Brontispa longissima were sequenced to identify and compare the expression patterns of genes involved in odorant reception and detection. Representative proteins from the chemosensory gene families likely essential for insect olfaction were identified. These include 48 odorant receptors (ORs), 19 ionotropic receptors (IRs), 4 sensory neuron membrane proteins (SNMPs), 34 odorant binding proteins (OBPs) and 16 chemosensory proteins (CSPs). Phylogenetic analysis revealed the evolutionary relationship of these proteins with homologs from Coleopterans or other insects, and led to the identification of putative aggregation pheromone receptors in B. longissima. Comparative expression analysis performed by calculating FPKM values were also validated using quantitative real time-PCR (qPCR). The results revealed that all ORs and antennal IRs, two IR co-receptors (BlonIR8a and BlonIR25a) and one SNMP (BlonSNMP1a) were predominantly expressed in antennae when compared to abdomens, and approximately half of the OBPs (19) and CSPs (7) were enriched in antennae. These findings for the first time reveal the identification of key molecular components in B. longissima olfaction and provide a valuable resource for future functional analyses of olfaction, and identification of potential targets to control this quarantine pest.Shu-Ying BinMeng-Qiu QuKe-Ming LiZheng-Qiang PengZhong-Zhen WuJin-Tian LinNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shu-Ying Bin Meng-Qiu Qu Ke-Ming Li Zheng-Qiang Peng Zhong-Zhen Wu Jin-Tian Lin Antennal and abdominal transcriptomes reveal chemosensory gene families in the coconut hispine beetle, Brontispa longissima
description	Abstract Antennal and abdominal transcriptomes of males and females of the coconut hispine beetle Brontispa longissima were sequenced to identify and compare the expression patterns of genes involved in odorant reception and detection. Representative proteins from the chemosensory gene families likely essential for insect olfaction were identified. These include 48 odorant receptors (ORs), 19 ionotropic receptors (IRs), 4 sensory neuron membrane proteins (SNMPs), 34 odorant binding proteins (OBPs) and 16 chemosensory proteins (CSPs). Phylogenetic analysis revealed the evolutionary relationship of these proteins with homologs from Coleopterans or other insects, and led to the identification of putative aggregation pheromone receptors in B. longissima. Comparative expression analysis performed by calculating FPKM values were also validated using quantitative real time-PCR (qPCR). The results revealed that all ORs and antennal IRs, two IR co-receptors (BlonIR8a and BlonIR25a) and one SNMP (BlonSNMP1a) were predominantly expressed in antennae when compared to abdomens, and approximately half of the OBPs (19) and CSPs (7) were enriched in antennae. These findings for the first time reveal the identification of key molecular components in B. longissima olfaction and provide a valuable resource for future functional analyses of olfaction, and identification of potential targets to control this quarantine pest.
format	article
author	Shu-Ying Bin Meng-Qiu Qu Ke-Ming Li Zheng-Qiang Peng Zhong-Zhen Wu Jin-Tian Lin
author_facet	Shu-Ying Bin Meng-Qiu Qu Ke-Ming Li Zheng-Qiang Peng Zhong-Zhen Wu Jin-Tian Lin
author_sort	Shu-Ying Bin
title	Antennal and abdominal transcriptomes reveal chemosensory gene families in the coconut hispine beetle, Brontispa longissima
title_short	Antennal and abdominal transcriptomes reveal chemosensory gene families in the coconut hispine beetle, Brontispa longissima
title_full	Antennal and abdominal transcriptomes reveal chemosensory gene families in the coconut hispine beetle, Brontispa longissima
title_fullStr	Antennal and abdominal transcriptomes reveal chemosensory gene families in the coconut hispine beetle, Brontispa longissima
title_full_unstemmed	Antennal and abdominal transcriptomes reveal chemosensory gene families in the coconut hispine beetle, Brontispa longissima
title_sort	antennal and abdominal transcriptomes reveal chemosensory gene families in the coconut hispine beetle, brontispa longissima
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/69b6ac78ace949e781843d9497779b58
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Antennal and abdominal transcriptomes reveal chemosensory gene families in the coconut hispine beetle, Brontispa longissima

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