Robust circadian clock oscillation and osmotic rhythms in inner medulla reflecting cortico-medullary osmotic gradient rhythm in rodent kidney

Abstract Circadian clocks in mammals function in most organs and tissues throughout the body. Various renal functions such as the glomerular filtration and excretion of electrolytes exhibit circadian rhythms. Although it has been reported that the expression of the clock genes composing molecular os...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Masayuki Hara, Yoichi Minami, Munehiro Ohashi, Yoshiki Tsuchiya, Tetsuro Kusaba, Keiichi Tamagaki, Nobuya Koike, Yasuhiro Umemura, Hitoshi Inokawa, Kazuhiro Yagita
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/69b90a5eabb74e1db275fed1e4d32f50
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:69b90a5eabb74e1db275fed1e4d32f50
record_format	dspace
spelling	oai:doaj.org-article:69b90a5eabb74e1db275fed1e4d32f502021-12-02T16:06:00ZRobust circadian clock oscillation and osmotic rhythms in inner medulla reflecting cortico-medullary osmotic gradient rhythm in rodent kidney10.1038/s41598-017-07767-82045-2322https://doaj.org/article/69b90a5eabb74e1db275fed1e4d32f502017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07767-8https://doaj.org/toc/2045-2322Abstract Circadian clocks in mammals function in most organs and tissues throughout the body. Various renal functions such as the glomerular filtration and excretion of electrolytes exhibit circadian rhythms. Although it has been reported that the expression of the clock genes composing molecular oscillators show apparent daily rhythms in rodent kidneys, functional variations of regional clocks are not yet fully understood. In this study, using macroscopic bioluminescence imaging method of the PER2::Luciferase knock-in mouse kidney, we reveal that strong and robust circadian clock oscillation is observed in the medulla. In addition, the osmotic pressure in the inner medulla shows apparent daily fluctuation, but not in the cortex. Quantitative-PCR analysis of the genes contributing to the generation of high osmotic pressure or the water re-absorption in the inner medulla, such as vasopressin receptors (V1aR, V2R), urea transporter (UT-A2) and water channel (Aqp2) show diurnal variations as well as clock genes. Deficiency of an essential clock gene Bmal1 impairs day-night variations of osmotic pressure gradient in the inner medulla, suggesting that circadian clocks in the medulla part of the kidney may regulate the circadian rhythm of cortico-medullary osmotic pressure gradient, and may contribute physiological day-night rhythm of urination.Masayuki HaraYoichi MinamiMunehiro OhashiYoshiki TsuchiyaTetsuro KusabaKeiichi TamagakiNobuya KoikeYasuhiro UmemuraHitoshi InokawaKazuhiro YagitaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Masayuki Hara Yoichi Minami Munehiro Ohashi Yoshiki Tsuchiya Tetsuro Kusaba Keiichi Tamagaki Nobuya Koike Yasuhiro Umemura Hitoshi Inokawa Kazuhiro Yagita Robust circadian clock oscillation and osmotic rhythms in inner medulla reflecting cortico-medullary osmotic gradient rhythm in rodent kidney
description	Abstract Circadian clocks in mammals function in most organs and tissues throughout the body. Various renal functions such as the glomerular filtration and excretion of electrolytes exhibit circadian rhythms. Although it has been reported that the expression of the clock genes composing molecular oscillators show apparent daily rhythms in rodent kidneys, functional variations of regional clocks are not yet fully understood. In this study, using macroscopic bioluminescence imaging method of the PER2::Luciferase knock-in mouse kidney, we reveal that strong and robust circadian clock oscillation is observed in the medulla. In addition, the osmotic pressure in the inner medulla shows apparent daily fluctuation, but not in the cortex. Quantitative-PCR analysis of the genes contributing to the generation of high osmotic pressure or the water re-absorption in the inner medulla, such as vasopressin receptors (V1aR, V2R), urea transporter (UT-A2) and water channel (Aqp2) show diurnal variations as well as clock genes. Deficiency of an essential clock gene Bmal1 impairs day-night variations of osmotic pressure gradient in the inner medulla, suggesting that circadian clocks in the medulla part of the kidney may regulate the circadian rhythm of cortico-medullary osmotic pressure gradient, and may contribute physiological day-night rhythm of urination.
format	article
author	Masayuki Hara Yoichi Minami Munehiro Ohashi Yoshiki Tsuchiya Tetsuro Kusaba Keiichi Tamagaki Nobuya Koike Yasuhiro Umemura Hitoshi Inokawa Kazuhiro Yagita
author_facet	Masayuki Hara Yoichi Minami Munehiro Ohashi Yoshiki Tsuchiya Tetsuro Kusaba Keiichi Tamagaki Nobuya Koike Yasuhiro Umemura Hitoshi Inokawa Kazuhiro Yagita
author_sort	Masayuki Hara
title	Robust circadian clock oscillation and osmotic rhythms in inner medulla reflecting cortico-medullary osmotic gradient rhythm in rodent kidney
title_short	Robust circadian clock oscillation and osmotic rhythms in inner medulla reflecting cortico-medullary osmotic gradient rhythm in rodent kidney
title_full	Robust circadian clock oscillation and osmotic rhythms in inner medulla reflecting cortico-medullary osmotic gradient rhythm in rodent kidney
title_fullStr	Robust circadian clock oscillation and osmotic rhythms in inner medulla reflecting cortico-medullary osmotic gradient rhythm in rodent kidney
title_full_unstemmed	Robust circadian clock oscillation and osmotic rhythms in inner medulla reflecting cortico-medullary osmotic gradient rhythm in rodent kidney
title_sort	robust circadian clock oscillation and osmotic rhythms in inner medulla reflecting cortico-medullary osmotic gradient rhythm in rodent kidney
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/69b90a5eabb74e1db275fed1e4d32f50
work_keys_str_mv	AT masayukihara robustcircadianclockoscillationandosmoticrhythmsininnermedullareflectingcorticomedullaryosmoticgradientrhythminrodentkidney AT yoichiminami robustcircadianclockoscillationandosmoticrhythmsininnermedullareflectingcorticomedullaryosmoticgradientrhythminrodentkidney AT munehiroohashi robustcircadianclockoscillationandosmoticrhythmsininnermedullareflectingcorticomedullaryosmoticgradientrhythminrodentkidney AT yoshikitsuchiya robustcircadianclockoscillationandosmoticrhythmsininnermedullareflectingcorticomedullaryosmoticgradientrhythminrodentkidney AT tetsurokusaba robustcircadianclockoscillationandosmoticrhythmsininnermedullareflectingcorticomedullaryosmoticgradientrhythminrodentkidney AT keiichitamagaki robustcircadianclockoscillationandosmoticrhythmsininnermedullareflectingcorticomedullaryosmoticgradientrhythminrodentkidney AT nobuyakoike robustcircadianclockoscillationandosmoticrhythmsininnermedullareflectingcorticomedullaryosmoticgradientrhythminrodentkidney AT yasuhiroumemura robustcircadianclockoscillationandosmoticrhythmsininnermedullareflectingcorticomedullaryosmoticgradientrhythminrodentkidney AT hitoshiinokawa robustcircadianclockoscillationandosmoticrhythmsininnermedullareflectingcorticomedullaryosmoticgradientrhythminrodentkidney AT kazuhiroyagita robustcircadianclockoscillationandosmoticrhythmsininnermedullareflectingcorticomedullaryosmoticgradientrhythminrodentkidney
_version_	1718385153963720704

Robust circadian clock oscillation and osmotic rhythms in inner medulla reflecting cortico-medullary osmotic gradient rhythm in rodent kidney

Ejemplares similares