Scutellarin-induced A549 cell apoptosis depends on activation of the transforming growth factor-β1/smad2/ROS/caspase-3 pathway

Scutellarin plays an anti-tumor role in A549 lung cancer cells, but the underlying mechanism is unclear. In this study, scutellarin was used to treat A549 cells for 12, 24, and 48 h, followed by the addition of Tempo, a selective scavenger of mitochondrial reactive oxygen species (ROS) and SB431542,...

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Autores principales:	Zhang Guang-Yan, Chen Wei-Yong, Li Xiao-Bo, Ke Hua, Zhou Xue-Lin
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2021
Materias:	scutellarin a549 cells tgf-β1/smad2 ros cleaved caspase-3 apoptosis Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/69bbaeb1f7fa43ec9e892f5c33f07fac
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id	oai:doaj.org-article:69bbaeb1f7fa43ec9e892f5c33f07fac
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spelling	oai:doaj.org-article:69bbaeb1f7fa43ec9e892f5c33f07fac2021-12-05T14:10:41ZScutellarin-induced A549 cell apoptosis depends on activation of the transforming growth factor-β1/smad2/ROS/caspase-3 pathway2391-541210.1515/biol-2021-0085https://doaj.org/article/69bbaeb1f7fa43ec9e892f5c33f07fac2021-09-01T00:00:00Zhttps://doi.org/10.1515/biol-2021-0085https://doaj.org/toc/2391-5412Scutellarin plays an anti-tumor role in A549 lung cancer cells, but the underlying mechanism is unclear. In this study, scutellarin was used to treat A549 cells for 12, 24, and 48 h, followed by the addition of Tempo, a selective scavenger of mitochondrial reactive oxygen species (ROS) and SB431542, a transforming growth factor (TGF)-β1 receptor inhibitor. A dihydroethidium fluorescence probe was used to measure the intracellular ROS level, Cell Counting Kit-8 (CCK-8) was used to detect cell viability, and flow cytometry was performed to examine apoptosis. Western blots were used to detect the total protein level of TGF-β1, p-smad2, and cleaved caspase-3 in A549 cells. The results showed that scutellarin significantly inhibited cell viability and increased apoptosis. Scutellarin also promoted intracellular ROS production, TGF-β1/smad2 signaling pathway activation, and cleaved caspase-3 expression, which was partly reversed by Tempo. Moreover, scutellarin-induced intracellular ROS production and cleaved caspase-3 expression were inhibited by blocking the TGF-β1/smad2 pathway with SB431542. In conclusion, scutellarin promoted apoptosis and intracellular ROS accumulation, which could be abrogated by Tempo and SB431542 treatment in A549 cells. Our study indicated that scutellarin induced A549 cell apoptosis via the TGF-β1/smad2/ROS/caspase-3 pathway.Zhang Guang-YanChen Wei-YongLi Xiao-BoKe HuaZhou Xue-LinDe Gruyterarticlescutellarina549 cellstgf-β1/smad2roscleaved caspase-3apoptosisBiology (General)QH301-705.5ENOpen Life Sciences, Vol 16, Iss 1, Pp 961-968 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	scutellarin a549 cells tgf-β1/smad2 ros cleaved caspase-3 apoptosis Biology (General) QH301-705.5
spellingShingle	scutellarin a549 cells tgf-β1/smad2 ros cleaved caspase-3 apoptosis Biology (General) QH301-705.5 Zhang Guang-Yan Chen Wei-Yong Li Xiao-Bo Ke Hua Zhou Xue-Lin Scutellarin-induced A549 cell apoptosis depends on activation of the transforming growth factor-β1/smad2/ROS/caspase-3 pathway
description	Scutellarin plays an anti-tumor role in A549 lung cancer cells, but the underlying mechanism is unclear. In this study, scutellarin was used to treat A549 cells for 12, 24, and 48 h, followed by the addition of Tempo, a selective scavenger of mitochondrial reactive oxygen species (ROS) and SB431542, a transforming growth factor (TGF)-β1 receptor inhibitor. A dihydroethidium fluorescence probe was used to measure the intracellular ROS level, Cell Counting Kit-8 (CCK-8) was used to detect cell viability, and flow cytometry was performed to examine apoptosis. Western blots were used to detect the total protein level of TGF-β1, p-smad2, and cleaved caspase-3 in A549 cells. The results showed that scutellarin significantly inhibited cell viability and increased apoptosis. Scutellarin also promoted intracellular ROS production, TGF-β1/smad2 signaling pathway activation, and cleaved caspase-3 expression, which was partly reversed by Tempo. Moreover, scutellarin-induced intracellular ROS production and cleaved caspase-3 expression were inhibited by blocking the TGF-β1/smad2 pathway with SB431542. In conclusion, scutellarin promoted apoptosis and intracellular ROS accumulation, which could be abrogated by Tempo and SB431542 treatment in A549 cells. Our study indicated that scutellarin induced A549 cell apoptosis via the TGF-β1/smad2/ROS/caspase-3 pathway.
format	article
author	Zhang Guang-Yan Chen Wei-Yong Li Xiao-Bo Ke Hua Zhou Xue-Lin
author_facet	Zhang Guang-Yan Chen Wei-Yong Li Xiao-Bo Ke Hua Zhou Xue-Lin
author_sort	Zhang Guang-Yan
title	Scutellarin-induced A549 cell apoptosis depends on activation of the transforming growth factor-β1/smad2/ROS/caspase-3 pathway
title_short	Scutellarin-induced A549 cell apoptosis depends on activation of the transforming growth factor-β1/smad2/ROS/caspase-3 pathway
title_full	Scutellarin-induced A549 cell apoptosis depends on activation of the transforming growth factor-β1/smad2/ROS/caspase-3 pathway
title_fullStr	Scutellarin-induced A549 cell apoptosis depends on activation of the transforming growth factor-β1/smad2/ROS/caspase-3 pathway
title_full_unstemmed	Scutellarin-induced A549 cell apoptosis depends on activation of the transforming growth factor-β1/smad2/ROS/caspase-3 pathway
title_sort	scutellarin-induced a549 cell apoptosis depends on activation of the transforming growth factor-β1/smad2/ros/caspase-3 pathway
publisher	De Gruyter
publishDate	2021
url	https://doaj.org/article/69bbaeb1f7fa43ec9e892f5c33f07fac
work_keys_str_mv	AT zhangguangyan scutellarininduceda549cellapoptosisdependsonactivationofthetransforminggrowthfactorb1smad2roscaspase3pathway AT chenweiyong scutellarininduceda549cellapoptosisdependsonactivationofthetransforminggrowthfactorb1smad2roscaspase3pathway AT lixiaobo scutellarininduceda549cellapoptosisdependsonactivationofthetransforminggrowthfactorb1smad2roscaspase3pathway AT kehua scutellarininduceda549cellapoptosisdependsonactivationofthetransforminggrowthfactorb1smad2roscaspase3pathway AT zhouxuelin scutellarininduceda549cellapoptosisdependsonactivationofthetransforminggrowthfactorb1smad2roscaspase3pathway
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Scutellarin-induced A549 cell apoptosis depends on activation of the transforming growth factor-β1/smad2/ROS/caspase-3 pathway

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