Double domain polyethylenimine-based nanoparticles for integrin receptor mediated delivery of plasmid DNA

Abstract The objective of the present study is to conjugate L-thyroxine PEI derivative onto another PEI to compensate the amine content of the whole structure which has been utilized for the ligand conjugation. Since αvβ3 integrin receptors are over-expressed on cancer cells and there is binding sit...

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Autores principales:	Hossein Sadeghpour, Bahman Khalvati, Elaheh Entezar-Almahdi, Narjes Savadi, Samira Hossaini Alhashemi, Mohammad Raoufi, Ali Dehshahri
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/69be0bdf915a4505a56c2e70a2ebd46a
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spelling	oai:doaj.org-article:69be0bdf915a4505a56c2e70a2ebd46a2021-12-02T16:08:03ZDouble domain polyethylenimine-based nanoparticles for integrin receptor mediated delivery of plasmid DNA10.1038/s41598-018-25277-z2045-2322https://doaj.org/article/69be0bdf915a4505a56c2e70a2ebd46a2018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25277-zhttps://doaj.org/toc/2045-2322Abstract The objective of the present study is to conjugate L-thyroxine PEI derivative onto another PEI to compensate the amine content of the whole structure which has been utilized for the ligand conjugation. Since αvβ3 integrin receptors are over-expressed on cancer cells and there is binding site for L-thyroxine on these receptors, PEI conjugation by L-thyroxine along with restoring the PEI amine content might be an efficient strategy for targeted delivery using polymeric nanoparticles. The results demonstrated the ability of the PEI conjugate in the formation of nanoparticles with the size of around 210 nm with higher buffering capacity. The conjugated PEI derivative increased the transfection efficiency in the cell lines over-expressing integrin by up to two folds higher than unmodified PEI, whereas in the cell lines lacking the integrin receptors there was no ligand conjugation-associated difference in gene transfer ability. The specificity of transfection demonstrated the delivery of plasmid DNA through integrin receptors. Also, the results of in vivo imaging of the polyplexes revealed that 99mTc-labeled PEI/plasmid DNA complexes accumulated in kidney and bladder 4 h post injection. Therefore, this PEI derivative could be considered as an efficient targeted delivery system for plasmid DNA.Hossein SadeghpourBahman KhalvatiElaheh Entezar-AlmahdiNarjes SavadiSamira Hossaini AlhashemiMohammad RaoufiAli DehshahriNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-12 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hossein Sadeghpour Bahman Khalvati Elaheh Entezar-Almahdi Narjes Savadi Samira Hossaini Alhashemi Mohammad Raoufi Ali Dehshahri Double domain polyethylenimine-based nanoparticles for integrin receptor mediated delivery of plasmid DNA
description	Abstract The objective of the present study is to conjugate L-thyroxine PEI derivative onto another PEI to compensate the amine content of the whole structure which has been utilized for the ligand conjugation. Since αvβ3 integrin receptors are over-expressed on cancer cells and there is binding site for L-thyroxine on these receptors, PEI conjugation by L-thyroxine along with restoring the PEI amine content might be an efficient strategy for targeted delivery using polymeric nanoparticles. The results demonstrated the ability of the PEI conjugate in the formation of nanoparticles with the size of around 210 nm with higher buffering capacity. The conjugated PEI derivative increased the transfection efficiency in the cell lines over-expressing integrin by up to two folds higher than unmodified PEI, whereas in the cell lines lacking the integrin receptors there was no ligand conjugation-associated difference in gene transfer ability. The specificity of transfection demonstrated the delivery of plasmid DNA through integrin receptors. Also, the results of in vivo imaging of the polyplexes revealed that 99mTc-labeled PEI/plasmid DNA complexes accumulated in kidney and bladder 4 h post injection. Therefore, this PEI derivative could be considered as an efficient targeted delivery system for plasmid DNA.
format	article
author	Hossein Sadeghpour Bahman Khalvati Elaheh Entezar-Almahdi Narjes Savadi Samira Hossaini Alhashemi Mohammad Raoufi Ali Dehshahri
author_facet	Hossein Sadeghpour Bahman Khalvati Elaheh Entezar-Almahdi Narjes Savadi Samira Hossaini Alhashemi Mohammad Raoufi Ali Dehshahri
author_sort	Hossein Sadeghpour
title	Double domain polyethylenimine-based nanoparticles for integrin receptor mediated delivery of plasmid DNA
title_short	Double domain polyethylenimine-based nanoparticles for integrin receptor mediated delivery of plasmid DNA
title_full	Double domain polyethylenimine-based nanoparticles for integrin receptor mediated delivery of plasmid DNA
title_fullStr	Double domain polyethylenimine-based nanoparticles for integrin receptor mediated delivery of plasmid DNA
title_full_unstemmed	Double domain polyethylenimine-based nanoparticles for integrin receptor mediated delivery of plasmid DNA
title_sort	double domain polyethylenimine-based nanoparticles for integrin receptor mediated delivery of plasmid dna
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/69be0bdf915a4505a56c2e70a2ebd46a
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Double domain polyethylenimine-based nanoparticles for integrin receptor mediated delivery of plasmid DNA

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