The role of histone methylation and H2A.Z occupancy during rapid activation of ethylene responsive genes.

Ethylene signaling pathway leads to rapid gene activation by two hierarchies of transcription factors with EIN3/EIL proteins as primary ones and ERF proteins as secondary ones. The role of chromatin modifications during the rapid gene activation is not known. In this work we studied trimethylated hi...

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Autores principales:	Yongfeng Hu, Yuan Shen, Natalia Conde E Silva, Dao-Xiu Zhou
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/69c6fb1dec514383b9b08027287e928e
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spelling	oai:doaj.org-article:69c6fb1dec514383b9b08027287e928e2021-11-18T07:33:30ZThe role of histone methylation and H2A.Z occupancy during rapid activation of ethylene responsive genes.1932-620310.1371/journal.pone.0028224https://doaj.org/article/69c6fb1dec514383b9b08027287e928e2011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22140554/?tool=EBIhttps://doaj.org/toc/1932-6203Ethylene signaling pathway leads to rapid gene activation by two hierarchies of transcription factors with EIN3/EIL proteins as primary ones and ERF proteins as secondary ones. The role of chromatin modifications during the rapid gene activation is not known. In this work we studied trimethylated histone H3 lysine 4 (H3K4me3) and lysine 27 (H3K27me3), two opposite histone methylation marks for gene activity, during the induction course of three ethylene-responsive genes (ERF1, AtERF14 and ChiB). We found that the three genes displayed different histone modification profiles before induction. After induction, H3K4me3 was increased in the 5' region and the gene body of ERF1, while H3K27me3 was decreased in the promoter of AtERF14. But the modification changes were later than the gene activation. Analysis of other rapidly inducible ERF genes confirmed the observation. In addition, histone H2A.Z occupancy on the three genes and the association of the H3K27me3-binding protein LHP1 with AtERF14 and ChiB were not affected by the inductive signal. However, the mutation of genes encoding H2A.Z and LHP1 attenuated and enhanced respectively the induction of target genes and altered H3K4me3. These results indicate that the induction of ethylene-responsive genes does not require immediate modulation of H3K4me3 and H3K27me3 and dissociation of LHP1 and H2A.Z from the targets, and suggest that the chromatin structure of the genes before induction is committed for transcriptional activation and that H3K4me3 is not required for ethylene-responsive gene activation, but may serve as a mark for gene activity.Yongfeng HuYuan ShenNatalia Conde E SilvaDao-Xiu ZhouPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 11, p e28224 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yongfeng Hu Yuan Shen Natalia Conde E Silva Dao-Xiu Zhou The role of histone methylation and H2A.Z occupancy during rapid activation of ethylene responsive genes.
description	Ethylene signaling pathway leads to rapid gene activation by two hierarchies of transcription factors with EIN3/EIL proteins as primary ones and ERF proteins as secondary ones. The role of chromatin modifications during the rapid gene activation is not known. In this work we studied trimethylated histone H3 lysine 4 (H3K4me3) and lysine 27 (H3K27me3), two opposite histone methylation marks for gene activity, during the induction course of three ethylene-responsive genes (ERF1, AtERF14 and ChiB). We found that the three genes displayed different histone modification profiles before induction. After induction, H3K4me3 was increased in the 5' region and the gene body of ERF1, while H3K27me3 was decreased in the promoter of AtERF14. But the modification changes were later than the gene activation. Analysis of other rapidly inducible ERF genes confirmed the observation. In addition, histone H2A.Z occupancy on the three genes and the association of the H3K27me3-binding protein LHP1 with AtERF14 and ChiB were not affected by the inductive signal. However, the mutation of genes encoding H2A.Z and LHP1 attenuated and enhanced respectively the induction of target genes and altered H3K4me3. These results indicate that the induction of ethylene-responsive genes does not require immediate modulation of H3K4me3 and H3K27me3 and dissociation of LHP1 and H2A.Z from the targets, and suggest that the chromatin structure of the genes before induction is committed for transcriptional activation and that H3K4me3 is not required for ethylene-responsive gene activation, but may serve as a mark for gene activity.
format	article
author	Yongfeng Hu Yuan Shen Natalia Conde E Silva Dao-Xiu Zhou
author_facet	Yongfeng Hu Yuan Shen Natalia Conde E Silva Dao-Xiu Zhou
author_sort	Yongfeng Hu
title	The role of histone methylation and H2A.Z occupancy during rapid activation of ethylene responsive genes.
title_short	The role of histone methylation and H2A.Z occupancy during rapid activation of ethylene responsive genes.
title_full	The role of histone methylation and H2A.Z occupancy during rapid activation of ethylene responsive genes.
title_fullStr	The role of histone methylation and H2A.Z occupancy during rapid activation of ethylene responsive genes.
title_full_unstemmed	The role of histone methylation and H2A.Z occupancy during rapid activation of ethylene responsive genes.
title_sort	role of histone methylation and h2a.z occupancy during rapid activation of ethylene responsive genes.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/69c6fb1dec514383b9b08027287e928e
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The role of histone methylation and H2A.Z occupancy during rapid activation of ethylene responsive genes.

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