SCREENING OF GENETIC MUTATION IN ABCA1 GENE AND RELATIONSHIP OF LOW-DENSITY LIPOPROTEIN AND TRIGLYCERIDES IN PATIENTS WITH TYPE 2 DIABETES WITH AND WITHOUT DYSLIPIDEMIA

Objective: To find the genetic mutation in exon-9 of the ABCA1 gene and relationship of serum low-density lipoprotein levels and serum triglycerides in patients with type 2 diabetes mellitus with and without dyslipidemia. Study Design: Cross-sectional comparative. Place and Duration of Study:...

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Autores principales:	Munnaza Sarfraz, Amir Rashid, Midhat Mustafa, Saleem Ahmad Khan, Asifa Majeed
Formato:	article
Lenguaje:	EN
Publicado:	Army Medical College Rawalpindi 2020
Materias:	abca1 gene dna sequencing dyslipidemia low-density lipoproteins triglycerides type 2 diabetes mellitus Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/69cd6c1490924d85b789cf5530fad7e5
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spelling	oai:doaj.org-article:69cd6c1490924d85b789cf5530fad7e52021-12-02T14:57:57ZSCREENING OF GENETIC MUTATION IN ABCA1 GENE AND RELATIONSHIP OF LOW-DENSITY LIPOPROTEIN AND TRIGLYCERIDES IN PATIENTS WITH TYPE 2 DIABETES WITH AND WITHOUT DYSLIPIDEMIA0030-96482411-8842https://doaj.org/article/69cd6c1490924d85b789cf5530fad7e52020-10-01T00:00:00Zhttps://pafmj.org/index.php/PAFMJ/article/view/5548https://doaj.org/toc/0030-9648https://doaj.org/toc/2411-8842Objective: To find the genetic mutation in exon-9 of the ABCA1 gene and relationship of serum low-density lipoprotein levels and serum triglycerides in patients with type 2 diabetes mellitus with and without dyslipidemia. Study Design: Cross-sectional comparative. Place and Duration of Study: Department of Biochemistry and Molecular Biology, Army Medical College Rawalpindi, from Jan to Dec 2017. Methodology: Ninety subjects were selected and divided into three groups, thirty in each. The division was based on newly diagnosed patients of type 2 diabetes with dyslipidemia, newly diagnosed patients of type 2 diabetes and healthy individuals. Genomic DNA was extracted from the blood samples of all subjects. Exon-9 of the ABCA1 gene was amplified through polymerase chain reaction and sequenced on automated DNA sequencer. The biochemical data was analyzed by SPSS-20 and presented in percentage and mean  SD. Results: The pattern of sequences of ABCA1 gene was found normal in patients of the group I and II through DNA sequencing. The levels of triglycerides were found elevated and mean value was 2.45 ± 0.57 for patients with diabetic dyslipidemia as compared to diabetic and control groups. The levels of low-density lipoproteins were observed as normal with mean value of 1.50 ± 0.22. Conclusion: The ABCA1 gene did not carry any genetic mutation in exon-9 in studied subjects. The raised level of triglyceride lipoproteins was a typical sign of dyslipidemia with a normal profile of low-density lipoproteinsMunnaza SarfrazAmir RashidMidhat MustafaSaleem Ahmad KhanAsifa MajeedArmy Medical College Rawalpindiarticleabca1 genedna sequencingdyslipidemialow-density lipoproteinstriglyceridestype 2 diabetes mellitusMedicineRMedicine (General)R5-920ENPakistan Armed Forces Medical Journal, Vol 70, Iss 5, Pp 1271-1276 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	abca1 gene dna sequencing dyslipidemia low-density lipoproteins triglycerides type 2 diabetes mellitus Medicine R Medicine (General) R5-920
spellingShingle	abca1 gene dna sequencing dyslipidemia low-density lipoproteins triglycerides type 2 diabetes mellitus Medicine R Medicine (General) R5-920 Munnaza Sarfraz Amir Rashid Midhat Mustafa Saleem Ahmad Khan Asifa Majeed SCREENING OF GENETIC MUTATION IN ABCA1 GENE AND RELATIONSHIP OF LOW-DENSITY LIPOPROTEIN AND TRIGLYCERIDES IN PATIENTS WITH TYPE 2 DIABETES WITH AND WITHOUT DYSLIPIDEMIA
description	Objective: To find the genetic mutation in exon-9 of the ABCA1 gene and relationship of serum low-density lipoprotein levels and serum triglycerides in patients with type 2 diabetes mellitus with and without dyslipidemia. Study Design: Cross-sectional comparative. Place and Duration of Study: Department of Biochemistry and Molecular Biology, Army Medical College Rawalpindi, from Jan to Dec 2017. Methodology: Ninety subjects were selected and divided into three groups, thirty in each. The division was based on newly diagnosed patients of type 2 diabetes with dyslipidemia, newly diagnosed patients of type 2 diabetes and healthy individuals. Genomic DNA was extracted from the blood samples of all subjects. Exon-9 of the ABCA1 gene was amplified through polymerase chain reaction and sequenced on automated DNA sequencer. The biochemical data was analyzed by SPSS-20 and presented in percentage and mean  SD. Results: The pattern of sequences of ABCA1 gene was found normal in patients of the group I and II through DNA sequencing. The levels of triglycerides were found elevated and mean value was 2.45 ± 0.57 for patients with diabetic dyslipidemia as compared to diabetic and control groups. The levels of low-density lipoproteins were observed as normal with mean value of 1.50 ± 0.22. Conclusion: The ABCA1 gene did not carry any genetic mutation in exon-9 in studied subjects. The raised level of triglyceride lipoproteins was a typical sign of dyslipidemia with a normal profile of low-density lipoproteins
format	article
author	Munnaza Sarfraz Amir Rashid Midhat Mustafa Saleem Ahmad Khan Asifa Majeed
author_facet	Munnaza Sarfraz Amir Rashid Midhat Mustafa Saleem Ahmad Khan Asifa Majeed
author_sort	Munnaza Sarfraz
title	SCREENING OF GENETIC MUTATION IN ABCA1 GENE AND RELATIONSHIP OF LOW-DENSITY LIPOPROTEIN AND TRIGLYCERIDES IN PATIENTS WITH TYPE 2 DIABETES WITH AND WITHOUT DYSLIPIDEMIA
title_short	SCREENING OF GENETIC MUTATION IN ABCA1 GENE AND RELATIONSHIP OF LOW-DENSITY LIPOPROTEIN AND TRIGLYCERIDES IN PATIENTS WITH TYPE 2 DIABETES WITH AND WITHOUT DYSLIPIDEMIA
title_full	SCREENING OF GENETIC MUTATION IN ABCA1 GENE AND RELATIONSHIP OF LOW-DENSITY LIPOPROTEIN AND TRIGLYCERIDES IN PATIENTS WITH TYPE 2 DIABETES WITH AND WITHOUT DYSLIPIDEMIA
title_fullStr	SCREENING OF GENETIC MUTATION IN ABCA1 GENE AND RELATIONSHIP OF LOW-DENSITY LIPOPROTEIN AND TRIGLYCERIDES IN PATIENTS WITH TYPE 2 DIABETES WITH AND WITHOUT DYSLIPIDEMIA
title_full_unstemmed	SCREENING OF GENETIC MUTATION IN ABCA1 GENE AND RELATIONSHIP OF LOW-DENSITY LIPOPROTEIN AND TRIGLYCERIDES IN PATIENTS WITH TYPE 2 DIABETES WITH AND WITHOUT DYSLIPIDEMIA
title_sort	screening of genetic mutation in abca1 gene and relationship of low-density lipoprotein and triglycerides in patients with type 2 diabetes with and without dyslipidemia
publisher	Army Medical College Rawalpindi
publishDate	2020
url	https://doaj.org/article/69cd6c1490924d85b789cf5530fad7e5
work_keys_str_mv	AT munnazasarfraz screeningofgeneticmutationinabca1geneandrelationshipoflowdensitylipoproteinandtriglyceridesinpatientswithtype2diabeteswithandwithoutdyslipidemia AT amirrashid screeningofgeneticmutationinabca1geneandrelationshipoflowdensitylipoproteinandtriglyceridesinpatientswithtype2diabeteswithandwithoutdyslipidemia AT midhatmustafa screeningofgeneticmutationinabca1geneandrelationshipoflowdensitylipoproteinandtriglyceridesinpatientswithtype2diabeteswithandwithoutdyslipidemia AT saleemahmadkhan screeningofgeneticmutationinabca1geneandrelationshipoflowdensitylipoproteinandtriglyceridesinpatientswithtype2diabeteswithandwithoutdyslipidemia AT asifamajeed screeningofgeneticmutationinabca1geneandrelationshipoflowdensitylipoproteinandtriglyceridesinpatientswithtype2diabeteswithandwithoutdyslipidemia
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SCREENING OF GENETIC MUTATION IN ABCA1 GENE AND RELATIONSHIP OF LOW-DENSITY LIPOPROTEIN AND TRIGLYCERIDES IN PATIENTS WITH TYPE 2 DIABETES WITH AND WITHOUT DYSLIPIDEMIA

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