Target identification of hepatic fibrosis using Pien Tze Huang based on mRNA and lncRNA

Abstract Hepatic fibrosis is a spontaneous wound-healing response triggered by chronic liver injury. Pien Tze Huang (PZH), a traditional Chinese herbal medicine, has been widely used to treat various hepatic diseases in Asia. We used a CCl4-induced mouse model to establish a PZH group of hepatic fib...

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Autores principales:	Jinhang Zhu, Di Zhang, Ting Wang, Zhiliang Chen, Luan Chen, Hao Wu, Cong Huai, Jing Sun, Na Zhang, Muyun Wei, Fei Hong, Shengying Qin
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/69d4a9e5f2e24b0eabdb7c1263263085
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spelling	oai:doaj.org-article:69d4a9e5f2e24b0eabdb7c12632630852021-12-02T18:51:52ZTarget identification of hepatic fibrosis using Pien Tze Huang based on mRNA and lncRNA10.1038/s41598-021-96459-52045-2322https://doaj.org/article/69d4a9e5f2e24b0eabdb7c12632630852021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96459-5https://doaj.org/toc/2045-2322Abstract Hepatic fibrosis is a spontaneous wound-healing response triggered by chronic liver injury. Pien Tze Huang (PZH), a traditional Chinese herbal medicine, has been widely used to treat various hepatic diseases in Asia. We used a CCl4-induced mouse model to establish a PZH group of hepatic fibrosis mice treated with PZH and a control group of hepatic fibrosis mice without any treatment. We performed RNA-seq and mass spectrometry sequencing to investigate the mechanism of the PZH response in hepatic fibrosis and identified multiple differentially expressed transcripts (DETs) and proteins (DEPs) that may be drug targets of PZH. Liver functional indices, including serum albumin (ALB), alanine aminotransferase (ALT) and aspartate aminotransferase (AST), were significantly decreased in the PZH treatment group (P < 0.05) in the eighth week. Hematoxylin–eosin (HE), Masson and Sirius red staining demonstrated that PZH significantly inhibited infiltration of inflammatory cells and collagen deposition. A total of 928 transcripts and 138 proteins were differentially expressed in PZH-treated mice compared to the control group. Gene Ontology (GO) enrichment analysis suggested that PZH may alleviate liver injury and fibrosis by enhancing the immune process. Taken together, our results revealed that multiple DETs and DEPs may serve as drug targets of PZH in hepatic fibrosis patient in future clinical practice.Jinhang ZhuDi ZhangTing WangZhiliang ChenLuan ChenHao WuCong HuaiJing SunNa ZhangMuyun WeiFei HongShengying QinNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jinhang Zhu Di Zhang Ting Wang Zhiliang Chen Luan Chen Hao Wu Cong Huai Jing Sun Na Zhang Muyun Wei Fei Hong Shengying Qin Target identification of hepatic fibrosis using Pien Tze Huang based on mRNA and lncRNA
description	Abstract Hepatic fibrosis is a spontaneous wound-healing response triggered by chronic liver injury. Pien Tze Huang (PZH), a traditional Chinese herbal medicine, has been widely used to treat various hepatic diseases in Asia. We used a CCl4-induced mouse model to establish a PZH group of hepatic fibrosis mice treated with PZH and a control group of hepatic fibrosis mice without any treatment. We performed RNA-seq and mass spectrometry sequencing to investigate the mechanism of the PZH response in hepatic fibrosis and identified multiple differentially expressed transcripts (DETs) and proteins (DEPs) that may be drug targets of PZH. Liver functional indices, including serum albumin (ALB), alanine aminotransferase (ALT) and aspartate aminotransferase (AST), were significantly decreased in the PZH treatment group (P < 0.05) in the eighth week. Hematoxylin–eosin (HE), Masson and Sirius red staining demonstrated that PZH significantly inhibited infiltration of inflammatory cells and collagen deposition. A total of 928 transcripts and 138 proteins were differentially expressed in PZH-treated mice compared to the control group. Gene Ontology (GO) enrichment analysis suggested that PZH may alleviate liver injury and fibrosis by enhancing the immune process. Taken together, our results revealed that multiple DETs and DEPs may serve as drug targets of PZH in hepatic fibrosis patient in future clinical practice.
format	article
author	Jinhang Zhu Di Zhang Ting Wang Zhiliang Chen Luan Chen Hao Wu Cong Huai Jing Sun Na Zhang Muyun Wei Fei Hong Shengying Qin
author_facet	Jinhang Zhu Di Zhang Ting Wang Zhiliang Chen Luan Chen Hao Wu Cong Huai Jing Sun Na Zhang Muyun Wei Fei Hong Shengying Qin
author_sort	Jinhang Zhu
title	Target identification of hepatic fibrosis using Pien Tze Huang based on mRNA and lncRNA
title_short	Target identification of hepatic fibrosis using Pien Tze Huang based on mRNA and lncRNA
title_full	Target identification of hepatic fibrosis using Pien Tze Huang based on mRNA and lncRNA
title_fullStr	Target identification of hepatic fibrosis using Pien Tze Huang based on mRNA and lncRNA
title_full_unstemmed	Target identification of hepatic fibrosis using Pien Tze Huang based on mRNA and lncRNA
title_sort	target identification of hepatic fibrosis using pien tze huang based on mrna and lncrna
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/69d4a9e5f2e24b0eabdb7c1263263085
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_version_	1718377389328695296

Target identification of hepatic fibrosis using Pien Tze Huang based on mRNA and lncRNA

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