Intranasal versus intratracheal exposure to lipopolysaccharides in a murine model of acute respiratory distress syndrome
Abstract Due to frequent and often severe lung affections caused by COVID-19, murine models of acute respiratory distress syndrome (ARDS) are increasingly used in experimental lung research. The one induced by a single lipopolysaccharide (LPS) exposure is practical. However, whether it is preferable...
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2021
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oai:doaj.org-article:69d659037d924acd87dd3dee7f86e3ac2021-12-02T18:15:09ZIntranasal versus intratracheal exposure to lipopolysaccharides in a murine model of acute respiratory distress syndrome10.1038/s41598-021-87462-x2045-2322https://doaj.org/article/69d659037d924acd87dd3dee7f86e3ac2021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87462-xhttps://doaj.org/toc/2045-2322Abstract Due to frequent and often severe lung affections caused by COVID-19, murine models of acute respiratory distress syndrome (ARDS) are increasingly used in experimental lung research. The one induced by a single lipopolysaccharide (LPS) exposure is practical. However, whether it is preferable to administer LPS intranasally or intratracheally remains an open question. Herein, female C57Bl/6 J mice were exposed intranasally or intratracheally to one dose of either saline or 3 mg/kg of LPS. They were studied 24 h later. The groups treated with LPS, either intranasally or intratracheally, exhibited a pronounced neutrophilic inflammation, signs of lung tissue damage and protein extravasation into the alveoli, and mild lung dysfunction. The magnitude of the response was generally not different between groups exposed intranasally versus intratracheally. However, the variability of some the responses was smaller in the LPS-treated groups exposed intranasally versus intratracheally. Notably, the saline-treated mice exposed intratracheally demonstrated a mild neutrophilic inflammation and alterations of the airway epithelium. We conclude that an intranasal exposure is as effective as an intratracheal exposure in a murine model of ARDS induced by LPS. Additionally, the groups exposed intranasally demonstrated less variability in the responses to LPS and less complications associated with the sham procedure.Fatemeh KhadangiAnne-Sophie ForguesSophie Tremblay-PitreAlexis Dufour-MailhotCyndi HenryMagali BoucherMarie-Josée BeaulieuMathieu MorissetteLiah FereydoonzadDavid BrunetAnnette RobichaudYnuk BosséNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021) |
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Medicine R Science Q Fatemeh Khadangi Anne-Sophie Forgues Sophie Tremblay-Pitre Alexis Dufour-Mailhot Cyndi Henry Magali Boucher Marie-Josée Beaulieu Mathieu Morissette Liah Fereydoonzad David Brunet Annette Robichaud Ynuk Bossé Intranasal versus intratracheal exposure to lipopolysaccharides in a murine model of acute respiratory distress syndrome |
| description |
Abstract Due to frequent and often severe lung affections caused by COVID-19, murine models of acute respiratory distress syndrome (ARDS) are increasingly used in experimental lung research. The one induced by a single lipopolysaccharide (LPS) exposure is practical. However, whether it is preferable to administer LPS intranasally or intratracheally remains an open question. Herein, female C57Bl/6 J mice were exposed intranasally or intratracheally to one dose of either saline or 3 mg/kg of LPS. They were studied 24 h later. The groups treated with LPS, either intranasally or intratracheally, exhibited a pronounced neutrophilic inflammation, signs of lung tissue damage and protein extravasation into the alveoli, and mild lung dysfunction. The magnitude of the response was generally not different between groups exposed intranasally versus intratracheally. However, the variability of some the responses was smaller in the LPS-treated groups exposed intranasally versus intratracheally. Notably, the saline-treated mice exposed intratracheally demonstrated a mild neutrophilic inflammation and alterations of the airway epithelium. We conclude that an intranasal exposure is as effective as an intratracheal exposure in a murine model of ARDS induced by LPS. Additionally, the groups exposed intranasally demonstrated less variability in the responses to LPS and less complications associated with the sham procedure. |
| format |
article |
| author |
Fatemeh Khadangi Anne-Sophie Forgues Sophie Tremblay-Pitre Alexis Dufour-Mailhot Cyndi Henry Magali Boucher Marie-Josée Beaulieu Mathieu Morissette Liah Fereydoonzad David Brunet Annette Robichaud Ynuk Bossé |
| author_facet |
Fatemeh Khadangi Anne-Sophie Forgues Sophie Tremblay-Pitre Alexis Dufour-Mailhot Cyndi Henry Magali Boucher Marie-Josée Beaulieu Mathieu Morissette Liah Fereydoonzad David Brunet Annette Robichaud Ynuk Bossé |
| author_sort |
Fatemeh Khadangi |
| title |
Intranasal versus intratracheal exposure to lipopolysaccharides in a murine model of acute respiratory distress syndrome |
| title_short |
Intranasal versus intratracheal exposure to lipopolysaccharides in a murine model of acute respiratory distress syndrome |
| title_full |
Intranasal versus intratracheal exposure to lipopolysaccharides in a murine model of acute respiratory distress syndrome |
| title_fullStr |
Intranasal versus intratracheal exposure to lipopolysaccharides in a murine model of acute respiratory distress syndrome |
| title_full_unstemmed |
Intranasal versus intratracheal exposure to lipopolysaccharides in a murine model of acute respiratory distress syndrome |
| title_sort |
intranasal versus intratracheal exposure to lipopolysaccharides in a murine model of acute respiratory distress syndrome |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/69d659037d924acd87dd3dee7f86e3ac |
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