A primary Chlamydia trachomatis genital infection of rhesus macaques identifies new immunodominant B-cell antigens.

To identify immunodominant antigens that elicit a humoral immune response following a primary and a secondary genital infection, rhesus monkeys were inoculated cervically with Chlamydia trachomatis serovar D. Serum samples were collected and probed with a protein microarray expressing 864/894 (96.4%...

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Autores principales:	Arlo Randall, Andy Teng, Xiaowu Liang, Sukumar Pal, Alice F Tarantal, Joseph Fike, Peter A Barry, Luis M de la Maza
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/69d6a9945e3245d8ad8f0cd058066446
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spelling	oai:doaj.org-article:69d6a9945e3245d8ad8f0cd0580664462021-12-02T20:05:43ZA primary Chlamydia trachomatis genital infection of rhesus macaques identifies new immunodominant B-cell antigens.1932-620310.1371/journal.pone.0250317https://doaj.org/article/69d6a9945e3245d8ad8f0cd0580664462021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0250317https://doaj.org/toc/1932-6203To identify immunodominant antigens that elicit a humoral immune response following a primary and a secondary genital infection, rhesus monkeys were inoculated cervically with Chlamydia trachomatis serovar D. Serum samples were collected and probed with a protein microarray expressing 864/894 (96.4%) of the open reading frames of the C. trachomatis serovar D genome. The antibody response to the primary infection was analyzed in 72 serum samples from 12 inoculated monkeys. The following criteria were utilized to identify immunodominant antigens: proteins found to be recognized by at least 75% (9/12) of the infected monkeys with at least 15% elevations in signal intensity from week 0 to week 8 post infection. All infected monkeys developed Chlamydia specific serum antibodies. Eight proteins satisfied the selection criteria for immunodominant antigens: CT242 (OmpH-like protein), CT541 (mip), CT681 (ompA), CT381 (artJ), CT443 (omcB), CT119 (incA), CT486 (fliY), and CT110 (groEL). Of these, three antigens, CT119, CT486 and CT381, were not previously identified as immunodominant antigens using non-human primate sera. Following the secondary infection, the antibody responses to the eight immunodominant antigens were analyzed and found to be quite different in intensity and duration to the primary infection. In conclusion, these eight immunodominant antigens can now be tested for their ability to identify individuals with a primary C. trachomatis genital infection and to design vaccine strategies to protect against a primary infection with this pathogen.Arlo RandallAndy TengXiaowu LiangSukumar PalAlice F TarantalJoseph FikePeter A BarryLuis M de la MazaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 4, p e0250317 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Arlo Randall Andy Teng Xiaowu Liang Sukumar Pal Alice F Tarantal Joseph Fike Peter A Barry Luis M de la Maza A primary Chlamydia trachomatis genital infection of rhesus macaques identifies new immunodominant B-cell antigens.
description	To identify immunodominant antigens that elicit a humoral immune response following a primary and a secondary genital infection, rhesus monkeys were inoculated cervically with Chlamydia trachomatis serovar D. Serum samples were collected and probed with a protein microarray expressing 864/894 (96.4%) of the open reading frames of the C. trachomatis serovar D genome. The antibody response to the primary infection was analyzed in 72 serum samples from 12 inoculated monkeys. The following criteria were utilized to identify immunodominant antigens: proteins found to be recognized by at least 75% (9/12) of the infected monkeys with at least 15% elevations in signal intensity from week 0 to week 8 post infection. All infected monkeys developed Chlamydia specific serum antibodies. Eight proteins satisfied the selection criteria for immunodominant antigens: CT242 (OmpH-like protein), CT541 (mip), CT681 (ompA), CT381 (artJ), CT443 (omcB), CT119 (incA), CT486 (fliY), and CT110 (groEL). Of these, three antigens, CT119, CT486 and CT381, were not previously identified as immunodominant antigens using non-human primate sera. Following the secondary infection, the antibody responses to the eight immunodominant antigens were analyzed and found to be quite different in intensity and duration to the primary infection. In conclusion, these eight immunodominant antigens can now be tested for their ability to identify individuals with a primary C. trachomatis genital infection and to design vaccine strategies to protect against a primary infection with this pathogen.
format	article
author	Arlo Randall Andy Teng Xiaowu Liang Sukumar Pal Alice F Tarantal Joseph Fike Peter A Barry Luis M de la Maza
author_facet	Arlo Randall Andy Teng Xiaowu Liang Sukumar Pal Alice F Tarantal Joseph Fike Peter A Barry Luis M de la Maza
author_sort	Arlo Randall
title	A primary Chlamydia trachomatis genital infection of rhesus macaques identifies new immunodominant B-cell antigens.
title_short	A primary Chlamydia trachomatis genital infection of rhesus macaques identifies new immunodominant B-cell antigens.
title_full	A primary Chlamydia trachomatis genital infection of rhesus macaques identifies new immunodominant B-cell antigens.
title_fullStr	A primary Chlamydia trachomatis genital infection of rhesus macaques identifies new immunodominant B-cell antigens.
title_full_unstemmed	A primary Chlamydia trachomatis genital infection of rhesus macaques identifies new immunodominant B-cell antigens.
title_sort	primary chlamydia trachomatis genital infection of rhesus macaques identifies new immunodominant b-cell antigens.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/69d6a9945e3245d8ad8f0cd058066446
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A primary Chlamydia trachomatis genital infection of rhesus macaques identifies new immunodominant B-cell antigens.

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