In vivo MR detection of fluorine-labeled human MSC using the bSSFP sequence

Emeline J Ribot,1 Jeffrey M Gaudet,1,2 Yuhua Chen,1 Kyle M Gilbert,1 Paula J Foster1,2 1Imaging Research Laboratories, Robarts Research Institute, London, ON, Canada; 2Department of Medical Biophysics, University of Western Ontario, London, ON, Canada Abstract: Mesenchymal stem cells (MSC) are use...

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Autores principales:	Ribot EJ, Gaudet JM, Chen Y, Gilbert KM, Foster PJ
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2014
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/69d6c57ead5840308fe1db93f740fe2f
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spelling	oai:doaj.org-article:69d6c57ead5840308fe1db93f740fe2f2021-12-02T03:54:52ZIn vivo MR detection of fluorine-labeled human MSC using the bSSFP sequence1178-2013https://doaj.org/article/69d6c57ead5840308fe1db93f740fe2f2014-04-01T00:00:00Zhttp://www.dovepress.com/in-vivo-mr-detection-of-fluorine-labeled-human-msc-using-the-bssfp-seq-a16390https://doaj.org/toc/1178-2013 Emeline J Ribot,1 Jeffrey M Gaudet,1,2 Yuhua Chen,1 Kyle M Gilbert,1 Paula J Foster1,2 1Imaging Research Laboratories, Robarts Research Institute, London, ON, Canada; 2Department of Medical Biophysics, University of Western Ontario, London, ON, Canada Abstract: Mesenchymal stem cells (MSC) are used to restore deteriorated cell environments. There is a need to specifically track these cells following transplantation in order to evaluate different methods of implantation, to follow their migration within the body, and to quantify their accumulation at the target. Cellular magnetic resonance imaging (MRI) using fluorine-based nanoemulsions is a great means to detect these transplanted cells in vivo because of the high specificity for fluorine detection and the capability for precise quantification. This technique, however, has low sensitivity, necessitating improvement in MR sequences. To counteract this issue, the balanced steady-state free precession (bSSFP) imaging sequence can be of great interest due to the high signal-to-noise ratio (SNR). Furthermore, it can be applied to obtain 3D images within short acquisition times. In this paper, bSSFP provided accurate quantification of samples of the perfluorocarbon Cell Sense-labeled cells in vitro. Cell Sense was internalized by human MSC (hMSC) without adverse alterations in cell viability or differentiation into adipocytes/osteocytes. The bSSFP sequence was applied in vivo to track and quantify the signals from both Cell Sense-labeled and iron-labeled hMSC after intramuscular implantation. The fluorine signal was observed to decrease faster and more significantly than the volume of iron-associated voids, which points to the advantage of quantifying the fluorine signal and the complexity of quantifying signal loss due to iron. Keywords: bSSFP, fluorine MRI, mesenchymal stem cell, mouse, cell trackingRibot EJGaudet JMChen YGilbert KMFoster PJDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 1731-1739 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Ribot EJ Gaudet JM Chen Y Gilbert KM Foster PJ In vivo MR detection of fluorine-labeled human MSC using the bSSFP sequence
description	Emeline J Ribot,1 Jeffrey M Gaudet,1,2 Yuhua Chen,1 Kyle M Gilbert,1 Paula J Foster1,2 1Imaging Research Laboratories, Robarts Research Institute, London, ON, Canada; 2Department of Medical Biophysics, University of Western Ontario, London, ON, Canada Abstract: Mesenchymal stem cells (MSC) are used to restore deteriorated cell environments. There is a need to specifically track these cells following transplantation in order to evaluate different methods of implantation, to follow their migration within the body, and to quantify their accumulation at the target. Cellular magnetic resonance imaging (MRI) using fluorine-based nanoemulsions is a great means to detect these transplanted cells in vivo because of the high specificity for fluorine detection and the capability for precise quantification. This technique, however, has low sensitivity, necessitating improvement in MR sequences. To counteract this issue, the balanced steady-state free precession (bSSFP) imaging sequence can be of great interest due to the high signal-to-noise ratio (SNR). Furthermore, it can be applied to obtain 3D images within short acquisition times. In this paper, bSSFP provided accurate quantification of samples of the perfluorocarbon Cell Sense-labeled cells in vitro. Cell Sense was internalized by human MSC (hMSC) without adverse alterations in cell viability or differentiation into adipocytes/osteocytes. The bSSFP sequence was applied in vivo to track and quantify the signals from both Cell Sense-labeled and iron-labeled hMSC after intramuscular implantation. The fluorine signal was observed to decrease faster and more significantly than the volume of iron-associated voids, which points to the advantage of quantifying the fluorine signal and the complexity of quantifying signal loss due to iron. Keywords: bSSFP, fluorine MRI, mesenchymal stem cell, mouse, cell tracking
format	article
author	Ribot EJ Gaudet JM Chen Y Gilbert KM Foster PJ
author_facet	Ribot EJ Gaudet JM Chen Y Gilbert KM Foster PJ
author_sort	Ribot EJ
title	In vivo MR detection of fluorine-labeled human MSC using the bSSFP sequence
title_short	In vivo MR detection of fluorine-labeled human MSC using the bSSFP sequence
title_full	In vivo MR detection of fluorine-labeled human MSC using the bSSFP sequence
title_fullStr	In vivo MR detection of fluorine-labeled human MSC using the bSSFP sequence
title_full_unstemmed	In vivo MR detection of fluorine-labeled human MSC using the bSSFP sequence
title_sort	in vivo mr detection of fluorine-labeled human msc using the bssfp sequence
publisher	Dove Medical Press
publishDate	2014
url	https://doaj.org/article/69d6c57ead5840308fe1db93f740fe2f
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_version_	1718401553941921792

In vivo MR detection of fluorine-labeled human MSC using the bSSFP sequence

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