Frequent combination of antimicrobial multiresistance and extraintestinal pathogenicity in Escherichia coli isolates from urban rats (Rattus norvegicus) in Berlin, Germany.

Urban rats present a global public health concern as they are considered a reservoir and vector of zoonotic pathogens, including Escherichia coli. In view of the increasing emergence of antimicrobial resistant E. coli strains and the on-going discussion about environmental reservoirs, we intended to...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Sebastian Guenther, Astrid Bethe, Angelika Fruth, Torsten Semmler, Rainer G Ulrich, Lothar H Wieler, Christa Ewers
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/69de0abcc16a49368526988a28bbe61e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:69de0abcc16a49368526988a28bbe61e
record_format	dspace
spelling	oai:doaj.org-article:69de0abcc16a49368526988a28bbe61e2021-11-18T08:07:34ZFrequent combination of antimicrobial multiresistance and extraintestinal pathogenicity in Escherichia coli isolates from urban rats (Rattus norvegicus) in Berlin, Germany.1932-620310.1371/journal.pone.0050331https://doaj.org/article/69de0abcc16a49368526988a28bbe61e2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23189197/?tool=EBIhttps://doaj.org/toc/1932-6203Urban rats present a global public health concern as they are considered a reservoir and vector of zoonotic pathogens, including Escherichia coli. In view of the increasing emergence of antimicrobial resistant E. coli strains and the on-going discussion about environmental reservoirs, we intended to analyse whether urban rats might be a potential source of putatively zoonotic E. coli combining resistance and virulence. For that, we took fecal samples from 87 brown rats (Rattus norvegicus) and tested at least three E. coli colonies from each animal. Thirty two of these E. coli strains were pre-selected from a total of 211 non-duplicate isolates based on their phenotypic resistance to at least three antimicrobial classes, thus fulfilling the definition of multiresistance. As determined by multilocus sequence typing (MLST), these 32 strains belonged to 24 different sequence types (STs), indicating a high phylogenetic diversity. We identified STs, which frequently occur among extraintestinal pathogenic E. coli (ExPEC), such as STs 95, 131, 70, 428, and 127. Also, the detection of a number of typical virulence genes confirmed that the rats tested carried ExPEC-like strains. In particular, the finding of an Extended-spectrum beta-lactamase (ESBL)-producing strain which belongs to a highly virulent, so far mainly human- and avian-restricted ExPEC lineage (ST95), which expresses a serogroup linked with invasive strains (O18:NM:K1), and finally, which produces an ESBL-type frequently identified among human strains (CTX-M-9), pointed towards the important role, urban rats might play in the transmission of multiresistant and virulent E. coli strains. Indeed, using a chicken infection model, this strain showed a high in vivo pathogenicity. Imagining the high numbers of urban rats living worldwide, the way to the transmission of putatively zoonotic, multiresistant, and virulent strains might not be far ahead. The unforeseeable consequences of such an emerging public health threat need careful consideration in the future.Sebastian GuentherAstrid BetheAngelika FruthTorsten SemmlerRainer G UlrichLothar H WielerChrista EwersPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 11, p e50331 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sebastian Guenther Astrid Bethe Angelika Fruth Torsten Semmler Rainer G Ulrich Lothar H Wieler Christa Ewers Frequent combination of antimicrobial multiresistance and extraintestinal pathogenicity in Escherichia coli isolates from urban rats (Rattus norvegicus) in Berlin, Germany.
description	Urban rats present a global public health concern as they are considered a reservoir and vector of zoonotic pathogens, including Escherichia coli. In view of the increasing emergence of antimicrobial resistant E. coli strains and the on-going discussion about environmental reservoirs, we intended to analyse whether urban rats might be a potential source of putatively zoonotic E. coli combining resistance and virulence. For that, we took fecal samples from 87 brown rats (Rattus norvegicus) and tested at least three E. coli colonies from each animal. Thirty two of these E. coli strains were pre-selected from a total of 211 non-duplicate isolates based on their phenotypic resistance to at least three antimicrobial classes, thus fulfilling the definition of multiresistance. As determined by multilocus sequence typing (MLST), these 32 strains belonged to 24 different sequence types (STs), indicating a high phylogenetic diversity. We identified STs, which frequently occur among extraintestinal pathogenic E. coli (ExPEC), such as STs 95, 131, 70, 428, and 127. Also, the detection of a number of typical virulence genes confirmed that the rats tested carried ExPEC-like strains. In particular, the finding of an Extended-spectrum beta-lactamase (ESBL)-producing strain which belongs to a highly virulent, so far mainly human- and avian-restricted ExPEC lineage (ST95), which expresses a serogroup linked with invasive strains (O18:NM:K1), and finally, which produces an ESBL-type frequently identified among human strains (CTX-M-9), pointed towards the important role, urban rats might play in the transmission of multiresistant and virulent E. coli strains. Indeed, using a chicken infection model, this strain showed a high in vivo pathogenicity. Imagining the high numbers of urban rats living worldwide, the way to the transmission of putatively zoonotic, multiresistant, and virulent strains might not be far ahead. The unforeseeable consequences of such an emerging public health threat need careful consideration in the future.
format	article
author	Sebastian Guenther Astrid Bethe Angelika Fruth Torsten Semmler Rainer G Ulrich Lothar H Wieler Christa Ewers
author_facet	Sebastian Guenther Astrid Bethe Angelika Fruth Torsten Semmler Rainer G Ulrich Lothar H Wieler Christa Ewers
author_sort	Sebastian Guenther
title	Frequent combination of antimicrobial multiresistance and extraintestinal pathogenicity in Escherichia coli isolates from urban rats (Rattus norvegicus) in Berlin, Germany.
title_short	Frequent combination of antimicrobial multiresistance and extraintestinal pathogenicity in Escherichia coli isolates from urban rats (Rattus norvegicus) in Berlin, Germany.
title_full	Frequent combination of antimicrobial multiresistance and extraintestinal pathogenicity in Escherichia coli isolates from urban rats (Rattus norvegicus) in Berlin, Germany.
title_fullStr	Frequent combination of antimicrobial multiresistance and extraintestinal pathogenicity in Escherichia coli isolates from urban rats (Rattus norvegicus) in Berlin, Germany.
title_full_unstemmed	Frequent combination of antimicrobial multiresistance and extraintestinal pathogenicity in Escherichia coli isolates from urban rats (Rattus norvegicus) in Berlin, Germany.
title_sort	frequent combination of antimicrobial multiresistance and extraintestinal pathogenicity in escherichia coli isolates from urban rats (rattus norvegicus) in berlin, germany.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/69de0abcc16a49368526988a28bbe61e
work_keys_str_mv	AT sebastianguenther frequentcombinationofantimicrobialmultiresistanceandextraintestinalpathogenicityinescherichiacoliisolatesfromurbanratsrattusnorvegicusinberlingermany AT astridbethe frequentcombinationofantimicrobialmultiresistanceandextraintestinalpathogenicityinescherichiacoliisolatesfromurbanratsrattusnorvegicusinberlingermany AT angelikafruth frequentcombinationofantimicrobialmultiresistanceandextraintestinalpathogenicityinescherichiacoliisolatesfromurbanratsrattusnorvegicusinberlingermany AT torstensemmler frequentcombinationofantimicrobialmultiresistanceandextraintestinalpathogenicityinescherichiacoliisolatesfromurbanratsrattusnorvegicusinberlingermany AT rainergulrich frequentcombinationofantimicrobialmultiresistanceandextraintestinalpathogenicityinescherichiacoliisolatesfromurbanratsrattusnorvegicusinberlingermany AT lotharhwieler frequentcombinationofantimicrobialmultiresistanceandextraintestinalpathogenicityinescherichiacoliisolatesfromurbanratsrattusnorvegicusinberlingermany AT christaewers frequentcombinationofantimicrobialmultiresistanceandextraintestinalpathogenicityinescherichiacoliisolatesfromurbanratsrattusnorvegicusinberlingermany
_version_	1718422172893970432

Frequent combination of antimicrobial multiresistance and extraintestinal pathogenicity in Escherichia coli isolates from urban rats (Rattus norvegicus) in Berlin, Germany.

Ejemplares similares