A Novel Colorimetric Nano Aptasensor for Ultrasensitive Detection of Aflatoxin B1 Based on the Exonuclease III-Assisted Signal Amplification Approach

The detection of aflatoxin B1 (AFB1) has recently garnered much attention on the issue of food safety. In this study, a novel and sensitive aptasensor towards AFB1 is proposed using an Exonuclease III (Exo III)-integrated signal amplification strategy. This reported sensing strategy is regulated by...

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Autores principales:	Yu Chen, Fuyuan Zhang, Ruobing Liu, Minxuan Liu, Yaxin Sang, Shuo Wang, Xianghong Wang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	aflatoxin B1 aptamer G-quadruplex Exonuclease III signal amplification Chemical technology TP1-1185
Acceso en línea:	https://doaj.org/article/69e340d25118412ba4ef4e8d05c78c79
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spelling	oai:doaj.org-article:69e340d25118412ba4ef4e8d05c78c792021-11-25T17:32:43ZA Novel Colorimetric Nano Aptasensor for Ultrasensitive Detection of Aflatoxin B1 Based on the Exonuclease III-Assisted Signal Amplification Approach10.3390/foods101125682304-8158https://doaj.org/article/69e340d25118412ba4ef4e8d05c78c792021-10-01T00:00:00Zhttps://www.mdpi.com/2304-8158/10/11/2568https://doaj.org/toc/2304-8158The detection of aflatoxin B1 (AFB1) has recently garnered much attention on the issue of food safety. In this study, a novel and sensitive aptasensor towards AFB1 is proposed using an Exonuclease III (Exo III)-integrated signal amplification strategy. This reported sensing strategy is regulated by aptamer-functionalized nanobeads that can target AFB1; furthermore, complementary DNA (cDNA) strands can lock the immobilized aptamer strands, preventing the signal amplification function of Exo III in the absence of AFB1. The presence of AFB1 triggers the displacement of cDNA, which will then activate the Exo III-integrated signal amplification procedure, resulting in the generation of a guanine (G)-rich sequence to form a G-4/hemin DNAzyme, which can catalyze the substrate of ABTS to produce a green color. Using this method, a practical detection limit of 0.0032 ng/mL and a dynamic range of detection from 0.0032 to 50 ng/mL were obtained. Additionally, the practical application of the established sensing method for AFB1 in complex matrices was demonstrated through recovery experiments. The recovery rate and relative standard deviations (RSD) in three kinds of cereal samples ranged from 93.83% to 111.58%, and 0.82% to 7.20%, respectively, which were comparable with or better than previously reported methods.Yu ChenFuyuan ZhangRuobing LiuMinxuan LiuYaxin SangShuo WangXianghong WangMDPI AGarticleaflatoxin B1aptamerG-quadruplexExonuclease IIIsignal amplificationChemical technologyTP1-1185ENFoods, Vol 10, Iss 2568, p 2568 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	aflatoxin B1 aptamer G-quadruplex Exonuclease III signal amplification Chemical technology TP1-1185
spellingShingle	aflatoxin B1 aptamer G-quadruplex Exonuclease III signal amplification Chemical technology TP1-1185 Yu Chen Fuyuan Zhang Ruobing Liu Minxuan Liu Yaxin Sang Shuo Wang Xianghong Wang A Novel Colorimetric Nano Aptasensor for Ultrasensitive Detection of Aflatoxin B1 Based on the Exonuclease III-Assisted Signal Amplification Approach
description	The detection of aflatoxin B1 (AFB1) has recently garnered much attention on the issue of food safety. In this study, a novel and sensitive aptasensor towards AFB1 is proposed using an Exonuclease III (Exo III)-integrated signal amplification strategy. This reported sensing strategy is regulated by aptamer-functionalized nanobeads that can target AFB1; furthermore, complementary DNA (cDNA) strands can lock the immobilized aptamer strands, preventing the signal amplification function of Exo III in the absence of AFB1. The presence of AFB1 triggers the displacement of cDNA, which will then activate the Exo III-integrated signal amplification procedure, resulting in the generation of a guanine (G)-rich sequence to form a G-4/hemin DNAzyme, which can catalyze the substrate of ABTS to produce a green color. Using this method, a practical detection limit of 0.0032 ng/mL and a dynamic range of detection from 0.0032 to 50 ng/mL were obtained. Additionally, the practical application of the established sensing method for AFB1 in complex matrices was demonstrated through recovery experiments. The recovery rate and relative standard deviations (RSD) in three kinds of cereal samples ranged from 93.83% to 111.58%, and 0.82% to 7.20%, respectively, which were comparable with or better than previously reported methods.
format	article
author	Yu Chen Fuyuan Zhang Ruobing Liu Minxuan Liu Yaxin Sang Shuo Wang Xianghong Wang
author_facet	Yu Chen Fuyuan Zhang Ruobing Liu Minxuan Liu Yaxin Sang Shuo Wang Xianghong Wang
author_sort	Yu Chen
title	A Novel Colorimetric Nano Aptasensor for Ultrasensitive Detection of Aflatoxin B1 Based on the Exonuclease III-Assisted Signal Amplification Approach
title_short	A Novel Colorimetric Nano Aptasensor for Ultrasensitive Detection of Aflatoxin B1 Based on the Exonuclease III-Assisted Signal Amplification Approach
title_full	A Novel Colorimetric Nano Aptasensor for Ultrasensitive Detection of Aflatoxin B1 Based on the Exonuclease III-Assisted Signal Amplification Approach
title_fullStr	A Novel Colorimetric Nano Aptasensor for Ultrasensitive Detection of Aflatoxin B1 Based on the Exonuclease III-Assisted Signal Amplification Approach
title_full_unstemmed	A Novel Colorimetric Nano Aptasensor for Ultrasensitive Detection of Aflatoxin B1 Based on the Exonuclease III-Assisted Signal Amplification Approach
title_sort	novel colorimetric nano aptasensor for ultrasensitive detection of aflatoxin b1 based on the exonuclease iii-assisted signal amplification approach
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/69e340d25118412ba4ef4e8d05c78c79
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A Novel Colorimetric Nano Aptasensor for Ultrasensitive Detection of Aflatoxin B1 Based on the Exonuclease III-Assisted Signal Amplification Approach

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