Nanoalumina induces apoptosis by impairing antioxidant enzyme systems in human hepatocarcinoma cells

Saud Alarifi, Daoud Ali, Saad Alkahtani Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia Abstract: Alumina nanoparticles (Al2O3NPs) are gradually used in various areas, including nanomedicine, biosensors, and electronics. The current study aimed to explore the...

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Autores principales:	Alarifi S, Ali D, Alkahtani S
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/69e41188d3ec4fb68168b20a5bf49a1e
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spelling	oai:doaj.org-article:69e41188d3ec4fb68168b20a5bf49a1e2021-12-02T01:45:10ZNanoalumina induces apoptosis by impairing antioxidant enzyme systems in human hepatocarcinoma cells1178-2013https://doaj.org/article/69e41188d3ec4fb68168b20a5bf49a1e2015-05-01T00:00:00Zhttp://www.dovepress.com/nanoalumina-induces-apoptosis-by-impairing-antioxidant-enzyme-systems--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Saud Alarifi, Daoud Ali, Saad Alkahtani Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia Abstract: Alumina nanoparticles (Al2O3NPs) are gradually used in various areas, including nanomedicine, biosensors, and electronics. The current study aimed to explore the DNA damage and cytotoxicity due to Al2O3NPs on human hepatocarcinoma cells (HepG2). The MTT and neutral red uptake assays showed that Al2O3NPs induce significant cell death in a dose- and time-dependent manner. However, Al2O3NPs induced significant intracellular reactive oxygen species production and elevated lipid peroxidation and superoxide dismutase levels in the HepG2 cells. Al2O3NPs also induced significant decrease in reduced glutathione levels and increase caspase-3 activity in HepG2 cells. DNA fragmentation analysis using the alkaline single-cell gel electrophoresis showed that Al2O3NPs cause genotoxicity in dose- and time-dependent manner. However, they induce reactive oxygen species production and oxidative stress, leading to oxidative DNA damage, a probable mechanism of genotoxicity. This study warrants more careful assessment of Al2O3NPs before their industrial application. Keywords: HepG2 cells, Al2O3NPs, oxidative stress, MTT assay, DNA damage Alarifi SAli DAlkahtani SDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 3751-3760 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Alarifi S Ali D Alkahtani S Nanoalumina induces apoptosis by impairing antioxidant enzyme systems in human hepatocarcinoma cells
description	Saud Alarifi, Daoud Ali, Saad Alkahtani Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia Abstract: Alumina nanoparticles (Al2O3NPs) are gradually used in various areas, including nanomedicine, biosensors, and electronics. The current study aimed to explore the DNA damage and cytotoxicity due to Al2O3NPs on human hepatocarcinoma cells (HepG2). The MTT and neutral red uptake assays showed that Al2O3NPs induce significant cell death in a dose- and time-dependent manner. However, Al2O3NPs induced significant intracellular reactive oxygen species production and elevated lipid peroxidation and superoxide dismutase levels in the HepG2 cells. Al2O3NPs also induced significant decrease in reduced glutathione levels and increase caspase-3 activity in HepG2 cells. DNA fragmentation analysis using the alkaline single-cell gel electrophoresis showed that Al2O3NPs cause genotoxicity in dose- and time-dependent manner. However, they induce reactive oxygen species production and oxidative stress, leading to oxidative DNA damage, a probable mechanism of genotoxicity. This study warrants more careful assessment of Al2O3NPs before their industrial application. Keywords: HepG2 cells, Al2O3NPs, oxidative stress, MTT assay, DNA damage
format	article
author	Alarifi S Ali D Alkahtani S
author_facet	Alarifi S Ali D Alkahtani S
author_sort	Alarifi S
title	Nanoalumina induces apoptosis by impairing antioxidant enzyme systems in human hepatocarcinoma cells
title_short	Nanoalumina induces apoptosis by impairing antioxidant enzyme systems in human hepatocarcinoma cells
title_full	Nanoalumina induces apoptosis by impairing antioxidant enzyme systems in human hepatocarcinoma cells
title_fullStr	Nanoalumina induces apoptosis by impairing antioxidant enzyme systems in human hepatocarcinoma cells
title_full_unstemmed	Nanoalumina induces apoptosis by impairing antioxidant enzyme systems in human hepatocarcinoma cells
title_sort	nanoalumina induces apoptosis by impairing antioxidant enzyme systems in human hepatocarcinoma cells
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/69e41188d3ec4fb68168b20a5bf49a1e
work_keys_str_mv	AT alarifis nanoaluminainducesapoptosisbyimpairingantioxidantenzymesystemsinhumanhepatocarcinomacells AT alid nanoaluminainducesapoptosisbyimpairingantioxidantenzymesystemsinhumanhepatocarcinomacells AT alkahtanis nanoaluminainducesapoptosisbyimpairingantioxidantenzymesystemsinhumanhepatocarcinomacells
_version_	1718402899754614784

Nanoalumina induces apoptosis by impairing antioxidant enzyme systems in human hepatocarcinoma cells

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