Pancreatic gene expression during recovery after pancreatitis reveals unique transcriptome profiles

Abstract It is well known that pancreatic recovery after a single episode of injury such as an isolated bout of pancreatitis occurs rapidly. It is unclear, however, what changes are inflicted in such conditions to the molecular landscape of the pancreas. In the caerulein hyperstimulation model of pa...

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Autores principales:	Kristy Boggs, Ting Wang, Abrahim I. Orabi, Amitava Mukherjee, John F. Eisses, Tao Sun, Li Wen, Tanveer A. Javed, Farzad Esni, Wei Chen, Sohail Z. Husain
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/69e80f3f1fcb461aaae46aed4750a6cc
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spelling	oai:doaj.org-article:69e80f3f1fcb461aaae46aed4750a6cc2021-12-02T15:08:12ZPancreatic gene expression during recovery after pancreatitis reveals unique transcriptome profiles10.1038/s41598-018-19392-02045-2322https://doaj.org/article/69e80f3f1fcb461aaae46aed4750a6cc2018-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-19392-0https://doaj.org/toc/2045-2322Abstract It is well known that pancreatic recovery after a single episode of injury such as an isolated bout of pancreatitis occurs rapidly. It is unclear, however, what changes are inflicted in such conditions to the molecular landscape of the pancreas. In the caerulein hyperstimulation model of pancreatitis, the murine pancreas has the ability to recover within one week based on histological appearance. In this study, we sought to characterize by RNA-sequencing (RNA-seq) the transcriptional profile of the recovering pancreas up to two weeks post-injury. We found that one week after injury there were 319 differentially expressed genes (DEGs) compared with baseline and that after two weeks there were 53 DEGs. Forty (12.5%) of the DEGs persisted from week one to week two, and another 13 DEGs newly emerged in the second week. Amongst the top up-regulated DEGs were several trypsinogen genes (trypsinogen 4, 5, 12, 15, and 16). To our knowledge, this is the first characterization of the transcriptome during pancreatic recovery by deep sequencing, and it reveals on a molecular basis that there is an ongoing recovery of the pancreas even after apparent histological resolution. The findings also raise the possibility of an emerging novel transcriptome upon pancreatic recovery.Kristy BoggsTing WangAbrahim I. OrabiAmitava MukherjeeJohn F. EissesTao SunLi WenTanveer A. JavedFarzad EsniWei ChenSohail Z. HusainNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-12 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kristy Boggs Ting Wang Abrahim I. Orabi Amitava Mukherjee John F. Eisses Tao Sun Li Wen Tanveer A. Javed Farzad Esni Wei Chen Sohail Z. Husain Pancreatic gene expression during recovery after pancreatitis reveals unique transcriptome profiles
description	Abstract It is well known that pancreatic recovery after a single episode of injury such as an isolated bout of pancreatitis occurs rapidly. It is unclear, however, what changes are inflicted in such conditions to the molecular landscape of the pancreas. In the caerulein hyperstimulation model of pancreatitis, the murine pancreas has the ability to recover within one week based on histological appearance. In this study, we sought to characterize by RNA-sequencing (RNA-seq) the transcriptional profile of the recovering pancreas up to two weeks post-injury. We found that one week after injury there were 319 differentially expressed genes (DEGs) compared with baseline and that after two weeks there were 53 DEGs. Forty (12.5%) of the DEGs persisted from week one to week two, and another 13 DEGs newly emerged in the second week. Amongst the top up-regulated DEGs were several trypsinogen genes (trypsinogen 4, 5, 12, 15, and 16). To our knowledge, this is the first characterization of the transcriptome during pancreatic recovery by deep sequencing, and it reveals on a molecular basis that there is an ongoing recovery of the pancreas even after apparent histological resolution. The findings also raise the possibility of an emerging novel transcriptome upon pancreatic recovery.
format	article
author	Kristy Boggs Ting Wang Abrahim I. Orabi Amitava Mukherjee John F. Eisses Tao Sun Li Wen Tanveer A. Javed Farzad Esni Wei Chen Sohail Z. Husain
author_facet	Kristy Boggs Ting Wang Abrahim I. Orabi Amitava Mukherjee John F. Eisses Tao Sun Li Wen Tanveer A. Javed Farzad Esni Wei Chen Sohail Z. Husain
author_sort	Kristy Boggs
title	Pancreatic gene expression during recovery after pancreatitis reveals unique transcriptome profiles
title_short	Pancreatic gene expression during recovery after pancreatitis reveals unique transcriptome profiles
title_full	Pancreatic gene expression during recovery after pancreatitis reveals unique transcriptome profiles
title_fullStr	Pancreatic gene expression during recovery after pancreatitis reveals unique transcriptome profiles
title_full_unstemmed	Pancreatic gene expression during recovery after pancreatitis reveals unique transcriptome profiles
title_sort	pancreatic gene expression during recovery after pancreatitis reveals unique transcriptome profiles
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/69e80f3f1fcb461aaae46aed4750a6cc
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Pancreatic gene expression during recovery after pancreatitis reveals unique transcriptome profiles

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