Comparing RAS with and without biofloc: transcriptional response of immune-related genes in Litopenaeus vannamei post-larvae

Background: Shrimp farming is evolving from semi-intensive to hyper-intensive systems with biofloc technology and water recirculation systems. Objective: To evaluate the transcriptional response promoted by biofloc on shrimp (Litopenaeus vannamei) under a recirculating aquaculture system (RAS). Meth...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jorge Soto Alcalá, Píndaro Álvarez Ruiz, Juan M. Audelo Naranjo, Héctor M. Esparza Leal, Irasema E. Luis Villaseñor, José A. Estrada-Godínez, Antonio Luna-González, Carina Gámez-Jiménez, Genaro Diarte Plata
Formato:	article
Lenguaje:	EN
Publicado:	Universidad de Antioquia 2020
Materias:	antimicrobial peptide aquaculture biofloc gene expression penaeidin ras shrimp shrimp immune system Animal culture SF1-1100
Acceso en línea:	https://doaj.org/article/69f2e7f9bfb34458b436a69c0d594c67
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:69f2e7f9bfb34458b436a69c0d594c67
record_format	dspace
spelling	oai:doaj.org-article:69f2e7f9bfb34458b436a69c0d594c672021-12-01T15:44:00ZComparing RAS with and without biofloc: transcriptional response of immune-related genes in Litopenaeus vannamei post-larvae2256-295810.17533/udea.rccp.v33n1a03https://doaj.org/article/69f2e7f9bfb34458b436a69c0d594c672020-01-01T00:00:00Zhttps://revistas.udea.edu.co/index.php/rccp/article/view/338302https://doaj.org/toc/2256-2958Background: Shrimp farming is evolving from semi-intensive to hyper-intensive systems with biofloc technology and water recirculation systems. Objective: To evaluate the transcriptional response promoted by biofloc on shrimp (Litopenaeus vannamei) under a recirculating aquaculture system (RAS). Methods: Quantitative real-time RT-PCR was used to monitor seven key genes related to the immune system in shrimp post-larvae, reared in a RAS with and without biofloc (BF and noBF). In addition, we present for the first time nucleotide sequences of ADP-ribosylation factor 4 (LvArf4) from Litopenaeus vannamei. Results: Transcripts for penaeidin3 (Pen3), penaeidin4 (Pen4), crustin, and Toll receptor (LvToll) genes were upregulated between 3 and 24 h in both systems, and tumor necrosis factor receptor-associated factor 6 (TRAF6) in no-BF as an early response. Regarding differential expression between treatments, 13 occurrences were encountered. Nine that were higher in BF than in no-BF and four higher in no-BF than in BF. In some sample times, expression of Pen3, crustin, LvToll, TRAF6, IMD, and LvArf4 was higher in BF than in no-BF and in others, expression of Pen3, Pen4, and TRAF6 was higher in no-BF than in BF. Conclusions: BF modulates the transcription of genes related to the immune response in shrimp as an early response. However, the RAS with no-BF promotes a similar response.Jorge Soto AlcaláPíndaro Álvarez RuizJuan M. Audelo NaranjoHéctor M. Esparza LealIrasema E. Luis VillaseñorJosé A. Estrada-GodínezAntonio Luna-GonzálezCarina Gámez-JiménezGenaro Diarte PlataUniversidad de Antioquiaarticleantimicrobial peptideaquaculturebioflocgene expressionpenaeidinrasshrimpshrimp immune systemAnimal cultureSF1-1100ENRevista Colombiana de Ciencias Pecuarias, Vol 33, Iss 1, Pp 32-43 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	antimicrobial peptide aquaculture biofloc gene expression penaeidin ras shrimp shrimp immune system Animal culture SF1-1100
spellingShingle	antimicrobial peptide aquaculture biofloc gene expression penaeidin ras shrimp shrimp immune system Animal culture SF1-1100 Jorge Soto Alcalá Píndaro Álvarez Ruiz Juan M. Audelo Naranjo Héctor M. Esparza Leal Irasema E. Luis Villaseñor José A. Estrada-Godínez Antonio Luna-González Carina Gámez-Jiménez Genaro Diarte Plata Comparing RAS with and without biofloc: transcriptional response of immune-related genes in Litopenaeus vannamei post-larvae
description	Background: Shrimp farming is evolving from semi-intensive to hyper-intensive systems with biofloc technology and water recirculation systems. Objective: To evaluate the transcriptional response promoted by biofloc on shrimp (Litopenaeus vannamei) under a recirculating aquaculture system (RAS). Methods: Quantitative real-time RT-PCR was used to monitor seven key genes related to the immune system in shrimp post-larvae, reared in a RAS with and without biofloc (BF and noBF). In addition, we present for the first time nucleotide sequences of ADP-ribosylation factor 4 (LvArf4) from Litopenaeus vannamei. Results: Transcripts for penaeidin3 (Pen3), penaeidin4 (Pen4), crustin, and Toll receptor (LvToll) genes were upregulated between 3 and 24 h in both systems, and tumor necrosis factor receptor-associated factor 6 (TRAF6) in no-BF as an early response. Regarding differential expression between treatments, 13 occurrences were encountered. Nine that were higher in BF than in no-BF and four higher in no-BF than in BF. In some sample times, expression of Pen3, crustin, LvToll, TRAF6, IMD, and LvArf4 was higher in BF than in no-BF and in others, expression of Pen3, Pen4, and TRAF6 was higher in no-BF than in BF. Conclusions: BF modulates the transcription of genes related to the immune response in shrimp as an early response. However, the RAS with no-BF promotes a similar response.
format	article
author	Jorge Soto Alcalá Píndaro Álvarez Ruiz Juan M. Audelo Naranjo Héctor M. Esparza Leal Irasema E. Luis Villaseñor José A. Estrada-Godínez Antonio Luna-González Carina Gámez-Jiménez Genaro Diarte Plata
author_facet	Jorge Soto Alcalá Píndaro Álvarez Ruiz Juan M. Audelo Naranjo Héctor M. Esparza Leal Irasema E. Luis Villaseñor José A. Estrada-Godínez Antonio Luna-González Carina Gámez-Jiménez Genaro Diarte Plata
author_sort	Jorge Soto Alcalá
title	Comparing RAS with and without biofloc: transcriptional response of immune-related genes in Litopenaeus vannamei post-larvae
title_short	Comparing RAS with and without biofloc: transcriptional response of immune-related genes in Litopenaeus vannamei post-larvae
title_full	Comparing RAS with and without biofloc: transcriptional response of immune-related genes in Litopenaeus vannamei post-larvae
title_fullStr	Comparing RAS with and without biofloc: transcriptional response of immune-related genes in Litopenaeus vannamei post-larvae
title_full_unstemmed	Comparing RAS with and without biofloc: transcriptional response of immune-related genes in Litopenaeus vannamei post-larvae
title_sort	comparing ras with and without biofloc: transcriptional response of immune-related genes in litopenaeus vannamei post-larvae
publisher	Universidad de Antioquia
publishDate	2020
url	https://doaj.org/article/69f2e7f9bfb34458b436a69c0d594c67
work_keys_str_mv	AT jorgesotoalcala comparingraswithandwithoutbiofloctranscriptionalresponseofimmunerelatedgenesinlitopenaeusvannameipostlarvae AT pindaroalvarezruiz comparingraswithandwithoutbiofloctranscriptionalresponseofimmunerelatedgenesinlitopenaeusvannameipostlarvae AT juanmaudelonaranjo comparingraswithandwithoutbiofloctranscriptionalresponseofimmunerelatedgenesinlitopenaeusvannameipostlarvae AT hectormesparzaleal comparingraswithandwithoutbiofloctranscriptionalresponseofimmunerelatedgenesinlitopenaeusvannameipostlarvae AT irasemaeluisvillasenor comparingraswithandwithoutbiofloctranscriptionalresponseofimmunerelatedgenesinlitopenaeusvannameipostlarvae AT joseaestradagodinez comparingraswithandwithoutbiofloctranscriptionalresponseofimmunerelatedgenesinlitopenaeusvannameipostlarvae AT antoniolunagonzalez comparingraswithandwithoutbiofloctranscriptionalresponseofimmunerelatedgenesinlitopenaeusvannameipostlarvae AT carinagamezjimenez comparingraswithandwithoutbiofloctranscriptionalresponseofimmunerelatedgenesinlitopenaeusvannameipostlarvae AT genarodiarteplata comparingraswithandwithoutbiofloctranscriptionalresponseofimmunerelatedgenesinlitopenaeusvannameipostlarvae
_version_	1718404820286570496

Comparing RAS with and without biofloc: transcriptional response of immune-related genes in Litopenaeus vannamei post-larvae

Ejemplares similares