Comparison of the modulatory effects of three structurally similar potential prebiotic substrates on an in vitro multi-species oral biofilm

Abstract Previous research identified potential prebiotic substrates for oral health like the structural analogues N-acetyl-d-mannosamine (NADM) and N-acetyl-d-glucosamine (NADG). The main hypothesis of the current study was twofold. Firstly, it was hypothesized that the modulatory effects of NADM a...

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Autores principales:	Tim Verspecht, Wannes Van Holm, Nico Boon, Kristel Bernaerts, Carlo A. Daep, Naiera Zayed, Marc Quirynen, Wim Teughels
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/69f8ecba5ebe45938dc04c614898f498
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spelling	oai:doaj.org-article:69f8ecba5ebe45938dc04c614898f4982021-12-02T16:26:30ZComparison of the modulatory effects of three structurally similar potential prebiotic substrates on an in vitro multi-species oral biofilm10.1038/s41598-021-94510-z2045-2322https://doaj.org/article/69f8ecba5ebe45938dc04c614898f4982021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94510-zhttps://doaj.org/toc/2045-2322Abstract Previous research identified potential prebiotic substrates for oral health like the structural analogues N-acetyl-d-mannosamine (NADM) and N-acetyl-d-glucosamine (NADG). The main hypothesis of the current study was twofold. Firstly, it was hypothesized that the modulatory effects of NADM are not limited to changes in multi-species oral biofilm composition, but also include effects on metabolism, virulence, and inflammatory potential. Secondly, the presence and orientation of their N-acetyl group could play a role. Therefore, a comparison was made between the effects of NADM, NADG and d-(+)-mannose on multi-species oral biofilms. Besides a beneficial compositional shift, NADM-treated biofilms also showed an altered metabolism, a reduced virulence and a decreased inflammatory potential. At a substrate concentration of 1 M, these effects were pronounced for all biofilm aspects, whereas at ~ 0.05 M (1%(w/v)) only the effects on virulence were pronounced. When comparing between substrates, both the presence and orientation of the N-acetyl group played a role. However, this was generally only at 1 M and dependent on the biofilm aspect. Overall, NADM was found to have different effects at two concentrations that beneficially modulate in vitro multi-species oral biofilm composition, metabolism, virulence and inflammatory potential. The presence and orientation of the N-acetyl group influenced these effects.Tim VerspechtWannes Van HolmNico BoonKristel BernaertsCarlo A. DaepNaiera ZayedMarc QuirynenWim TeughelsNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tim Verspecht Wannes Van Holm Nico Boon Kristel Bernaerts Carlo A. Daep Naiera Zayed Marc Quirynen Wim Teughels Comparison of the modulatory effects of three structurally similar potential prebiotic substrates on an in vitro multi-species oral biofilm
description	Abstract Previous research identified potential prebiotic substrates for oral health like the structural analogues N-acetyl-d-mannosamine (NADM) and N-acetyl-d-glucosamine (NADG). The main hypothesis of the current study was twofold. Firstly, it was hypothesized that the modulatory effects of NADM are not limited to changes in multi-species oral biofilm composition, but also include effects on metabolism, virulence, and inflammatory potential. Secondly, the presence and orientation of their N-acetyl group could play a role. Therefore, a comparison was made between the effects of NADM, NADG and d-(+)-mannose on multi-species oral biofilms. Besides a beneficial compositional shift, NADM-treated biofilms also showed an altered metabolism, a reduced virulence and a decreased inflammatory potential. At a substrate concentration of 1 M, these effects were pronounced for all biofilm aspects, whereas at ~ 0.05 M (1%(w/v)) only the effects on virulence were pronounced. When comparing between substrates, both the presence and orientation of the N-acetyl group played a role. However, this was generally only at 1 M and dependent on the biofilm aspect. Overall, NADM was found to have different effects at two concentrations that beneficially modulate in vitro multi-species oral biofilm composition, metabolism, virulence and inflammatory potential. The presence and orientation of the N-acetyl group influenced these effects.
format	article
author	Tim Verspecht Wannes Van Holm Nico Boon Kristel Bernaerts Carlo A. Daep Naiera Zayed Marc Quirynen Wim Teughels
author_facet	Tim Verspecht Wannes Van Holm Nico Boon Kristel Bernaerts Carlo A. Daep Naiera Zayed Marc Quirynen Wim Teughels
author_sort	Tim Verspecht
title	Comparison of the modulatory effects of three structurally similar potential prebiotic substrates on an in vitro multi-species oral biofilm
title_short	Comparison of the modulatory effects of three structurally similar potential prebiotic substrates on an in vitro multi-species oral biofilm
title_full	Comparison of the modulatory effects of three structurally similar potential prebiotic substrates on an in vitro multi-species oral biofilm
title_fullStr	Comparison of the modulatory effects of three structurally similar potential prebiotic substrates on an in vitro multi-species oral biofilm
title_full_unstemmed	Comparison of the modulatory effects of three structurally similar potential prebiotic substrates on an in vitro multi-species oral biofilm
title_sort	comparison of the modulatory effects of three structurally similar potential prebiotic substrates on an in vitro multi-species oral biofilm
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/69f8ecba5ebe45938dc04c614898f498
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Comparison of the modulatory effects of three structurally similar potential prebiotic substrates on an in vitro multi-species oral biofilm

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