Enzymatic Synthesis of Ricinoleyl Hydroxamic Acid Based on Commercial Castor Oil, Cytotoxicity Properties and Application as a New Anticancer Agent

Hossein Jahangirian,1 Bahram Saleh,1 Katayoon Kalantari,1 Roshanak Rafiee-Moghaddam,1 Bahareh Nikpey,2 Siavash Jahangirian,3 Thomas J Webster1 1Department of Chemical Engineering, College of Engineering, Northeastern University, Boston, MA 02115, USA; 2Department of Agronomy and Plant Breeding, Facu...

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Autores principales:	Jahangirian H, Saleh B, Kalantari K, Rafiee-Moghaddam R, Nikpey B, Jahangirian S, Webster TJ
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	ricinoleyl hydroxamic acid enzymatic reaction anticancer agent biomedicine lipozyme and castor oil Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/69fbfe41c9454fb499f478364b8b9a25
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spelling	oai:doaj.org-article:69fbfe41c9454fb499f478364b8b9a252021-12-02T08:54:58ZEnzymatic Synthesis of Ricinoleyl Hydroxamic Acid Based on Commercial Castor Oil, Cytotoxicity Properties and Application as a New Anticancer Agent1178-2013https://doaj.org/article/69fbfe41c9454fb499f478364b8b9a252020-04-01T00:00:00Zhttps://www.dovepress.com/enzymatic-synthesis-of-ricinoleyl-hydroxamic-acid-based-on-commercial--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Hossein Jahangirian,1 Bahram Saleh,1 Katayoon Kalantari,1 Roshanak Rafiee-Moghaddam,1 Bahareh Nikpey,2 Siavash Jahangirian,3 Thomas J Webster1 1Department of Chemical Engineering, College of Engineering, Northeastern University, Boston, MA 02115, USA; 2Department of Agronomy and Plant Breeding, Faculty of Engineering and Agriculture, Science and Research Branch, IA University, Tehran, Iran; 3Department of Agronomy, Faculty of Agriculture, IA University, Tehran, IranCorrespondence: Hossein Jahangirian; Thomas J Webster Email kamran.jahangirian@gmail.com; th.webster@neu.eduBackground: New anticancer agents that rely on natural/healthy, not synthetic/toxic, components are very much needed.Methods: Ricinoleyl hydroxamic acid (RHA) was synthesized from castor oil and hydroxylamine using Lipozyme TL IM as a catalyst. To optimize the conversion, the effects of the following parameters were investigated: type of organic solvent, period of reaction, amount of enzyme, the molar ratio of reactants and temperature. The highest conversion was obtained when the reaction was carried out under the following conditions: hexane as a solvent; reaction period of 48 hours; 120 mg of Lipozyme TL IM/3 mmol oil; HA-oil ratio of 19 mmol HA/3 mmol oil; and temperature of 40°C. The cytotoxicity of the synthesized RHA was assessed using human dermal fibroblasts (HDF), and its application towards fighting cancer was assessed using melanoma and glioblastoma cancer cells over a duration of 24 and 48 hours.Results: RHA was successfully synthesized  and it demonstrated strong anticancer activity against glioblastoma and melanoma cells at as low as a 1 μg/mL concentration while it did not demonstrate any toxicity against HDF cells.Conclusion: This is the first report on the synthesis of RHA with great potential to be used as a new anticancer agent.Keywords: ricinoleyl hydroxamic acid, enzymatic reaction, anticancer agent, biomedicine, lipozyme and castor oilJahangirian HSaleh BKalantari KRafiee-Moghaddam RNikpey BJahangirian SWebster TJDove Medical Pressarticlericinoleyl hydroxamic acidenzymatic reactionanticancer agentbiomedicinelipozyme and castor oilMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 2935-2945 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	ricinoleyl hydroxamic acid enzymatic reaction anticancer agent biomedicine lipozyme and castor oil Medicine (General) R5-920
spellingShingle	ricinoleyl hydroxamic acid enzymatic reaction anticancer agent biomedicine lipozyme and castor oil Medicine (General) R5-920 Jahangirian H Saleh B Kalantari K Rafiee-Moghaddam R Nikpey B Jahangirian S Webster TJ Enzymatic Synthesis of Ricinoleyl Hydroxamic Acid Based on Commercial Castor Oil, Cytotoxicity Properties and Application as a New Anticancer Agent
description	Hossein Jahangirian,1 Bahram Saleh,1 Katayoon Kalantari,1 Roshanak Rafiee-Moghaddam,1 Bahareh Nikpey,2 Siavash Jahangirian,3 Thomas J Webster1 1Department of Chemical Engineering, College of Engineering, Northeastern University, Boston, MA 02115, USA; 2Department of Agronomy and Plant Breeding, Faculty of Engineering and Agriculture, Science and Research Branch, IA University, Tehran, Iran; 3Department of Agronomy, Faculty of Agriculture, IA University, Tehran, IranCorrespondence: Hossein Jahangirian; Thomas J Webster Email kamran.jahangirian@gmail.com; th.webster@neu.eduBackground: New anticancer agents that rely on natural/healthy, not synthetic/toxic, components are very much needed.Methods: Ricinoleyl hydroxamic acid (RHA) was synthesized from castor oil and hydroxylamine using Lipozyme TL IM as a catalyst. To optimize the conversion, the effects of the following parameters were investigated: type of organic solvent, period of reaction, amount of enzyme, the molar ratio of reactants and temperature. The highest conversion was obtained when the reaction was carried out under the following conditions: hexane as a solvent; reaction period of 48 hours; 120 mg of Lipozyme TL IM/3 mmol oil; HA-oil ratio of 19 mmol HA/3 mmol oil; and temperature of 40°C. The cytotoxicity of the synthesized RHA was assessed using human dermal fibroblasts (HDF), and its application towards fighting cancer was assessed using melanoma and glioblastoma cancer cells over a duration of 24 and 48 hours.Results: RHA was successfully synthesized  and it demonstrated strong anticancer activity against glioblastoma and melanoma cells at as low as a 1 μg/mL concentration while it did not demonstrate any toxicity against HDF cells.Conclusion: This is the first report on the synthesis of RHA with great potential to be used as a new anticancer agent.Keywords: ricinoleyl hydroxamic acid, enzymatic reaction, anticancer agent, biomedicine, lipozyme and castor oil
format	article
author	Jahangirian H Saleh B Kalantari K Rafiee-Moghaddam R Nikpey B Jahangirian S Webster TJ
author_facet	Jahangirian H Saleh B Kalantari K Rafiee-Moghaddam R Nikpey B Jahangirian S Webster TJ
author_sort	Jahangirian H
title	Enzymatic Synthesis of Ricinoleyl Hydroxamic Acid Based on Commercial Castor Oil, Cytotoxicity Properties and Application as a New Anticancer Agent
title_short	Enzymatic Synthesis of Ricinoleyl Hydroxamic Acid Based on Commercial Castor Oil, Cytotoxicity Properties and Application as a New Anticancer Agent
title_full	Enzymatic Synthesis of Ricinoleyl Hydroxamic Acid Based on Commercial Castor Oil, Cytotoxicity Properties and Application as a New Anticancer Agent
title_fullStr	Enzymatic Synthesis of Ricinoleyl Hydroxamic Acid Based on Commercial Castor Oil, Cytotoxicity Properties and Application as a New Anticancer Agent
title_full_unstemmed	Enzymatic Synthesis of Ricinoleyl Hydroxamic Acid Based on Commercial Castor Oil, Cytotoxicity Properties and Application as a New Anticancer Agent
title_sort	enzymatic synthesis of ricinoleyl hydroxamic acid based on commercial castor oil, cytotoxicity properties and application as a new anticancer agent
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/69fbfe41c9454fb499f478364b8b9a25
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Enzymatic Synthesis of Ricinoleyl Hydroxamic Acid Based on Commercial Castor Oil, Cytotoxicity Properties and Application as a New Anticancer Agent

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