Banking of AT-MSC and its Influence on Their Application to Clinical Procedures

Processing of MSCs to obtain a therapeutic product consists of two main steps: 1) the in vitro expansion of the cells until an appropriate number of them is obtained, and 2) freezing and storage of the expanded cells. The last step is critical and must be optimized so that after thawing the cells re...

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Autores principales:	Ekaterina Semenova, Mariusz P. Grudniak, Katarzyna Bocian, Magdalena Chroscinska-Krawczyk, Marzena Trochonowicz, Igor M. Stepaniec, Magdalena Murzyn, Ilona Szablowska-Gadomska, Dariusz Boruczkowski, Tomasz Oldak, Eugeniusz K. Machaj
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	cryopreservation banking cryoprotectant ADSC application Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/69fcf72aa9d34a73b3812723737a733f
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spelling	oai:doaj.org-article:69fcf72aa9d34a73b3812723737a733f2021-12-01T20:06:09ZBanking of AT-MSC and its Influence on Their Application to Clinical Procedures2296-418510.3389/fbioe.2021.773123https://doaj.org/article/69fcf72aa9d34a73b3812723737a733f2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fbioe.2021.773123/fullhttps://doaj.org/toc/2296-4185Processing of MSCs to obtain a therapeutic product consists of two main steps: 1) the in vitro expansion of the cells until an appropriate number of them is obtained, and 2) freezing and storage of the expanded cells. The last step is critical and must be optimized so that after thawing the cells retain all their physiological properties including the secretory function. In this paper, we evaluated physiological parameters of AT-MSC’s after a full cycle of their processing, particularly freezing and storing at the liquid nitrogen vapor temperature. Based on the recovered proliferative and secretory capacities of the thawed cells, we have designed the optimal technique for processing of MSCs for clinical applications. In our work, we tried to select the best DMSO-based cryoprotectant mixture on the base of post thawing fully retain their properties. We have demonstrated the effectiveness of the use of DMSO in various configurations of the constituent cryoprotective fluids. We have also shown that AT-MSCs that show control levels in most standard tests (viability, shape, culture behaviour, and proliferative properties) after thawing, may show transient variations in some important physiological properties, such as the level of secreted growth factors. Obtained results let us to indicate how to optimize the AT-MSC preparation process for clinical applications. We suggest that before their clinical application the cells should be cultured for at least one passage to recover their physiological stability and thus assure their optimal therapeutic potential.Ekaterina SemenovaMariusz P. GrudniakKatarzyna BocianKatarzyna BocianMagdalena Chroscinska-KrawczykMarzena TrochonowiczIgor M. StepaniecMagdalena MurzynMagdalena MurzynIlona Szablowska-GadomskaDariusz BoruczkowskiTomasz OldakEugeniusz K. MachajFrontiers Media S.A.articlecryopreservationbankingcryoprotectantADSCapplicationBiotechnologyTP248.13-248.65ENFrontiers in Bioengineering and Biotechnology, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cryopreservation banking cryoprotectant ADSC application Biotechnology TP248.13-248.65
spellingShingle	cryopreservation banking cryoprotectant ADSC application Biotechnology TP248.13-248.65 Ekaterina Semenova Mariusz P. Grudniak Katarzyna Bocian Katarzyna Bocian Magdalena Chroscinska-Krawczyk Marzena Trochonowicz Igor M. Stepaniec Magdalena Murzyn Magdalena Murzyn Ilona Szablowska-Gadomska Dariusz Boruczkowski Tomasz Oldak Eugeniusz K. Machaj Banking of AT-MSC and its Influence on Their Application to Clinical Procedures
description	Processing of MSCs to obtain a therapeutic product consists of two main steps: 1) the in vitro expansion of the cells until an appropriate number of them is obtained, and 2) freezing and storage of the expanded cells. The last step is critical and must be optimized so that after thawing the cells retain all their physiological properties including the secretory function. In this paper, we evaluated physiological parameters of AT-MSC’s after a full cycle of their processing, particularly freezing and storing at the liquid nitrogen vapor temperature. Based on the recovered proliferative and secretory capacities of the thawed cells, we have designed the optimal technique for processing of MSCs for clinical applications. In our work, we tried to select the best DMSO-based cryoprotectant mixture on the base of post thawing fully retain their properties. We have demonstrated the effectiveness of the use of DMSO in various configurations of the constituent cryoprotective fluids. We have also shown that AT-MSCs that show control levels in most standard tests (viability, shape, culture behaviour, and proliferative properties) after thawing, may show transient variations in some important physiological properties, such as the level of secreted growth factors. Obtained results let us to indicate how to optimize the AT-MSC preparation process for clinical applications. We suggest that before their clinical application the cells should be cultured for at least one passage to recover their physiological stability and thus assure their optimal therapeutic potential.
format	article
author	Ekaterina Semenova Mariusz P. Grudniak Katarzyna Bocian Katarzyna Bocian Magdalena Chroscinska-Krawczyk Marzena Trochonowicz Igor M. Stepaniec Magdalena Murzyn Magdalena Murzyn Ilona Szablowska-Gadomska Dariusz Boruczkowski Tomasz Oldak Eugeniusz K. Machaj
author_facet	Ekaterina Semenova Mariusz P. Grudniak Katarzyna Bocian Katarzyna Bocian Magdalena Chroscinska-Krawczyk Marzena Trochonowicz Igor M. Stepaniec Magdalena Murzyn Magdalena Murzyn Ilona Szablowska-Gadomska Dariusz Boruczkowski Tomasz Oldak Eugeniusz K. Machaj
author_sort	Ekaterina Semenova
title	Banking of AT-MSC and its Influence on Their Application to Clinical Procedures
title_short	Banking of AT-MSC and its Influence on Their Application to Clinical Procedures
title_full	Banking of AT-MSC and its Influence on Their Application to Clinical Procedures
title_fullStr	Banking of AT-MSC and its Influence on Their Application to Clinical Procedures
title_full_unstemmed	Banking of AT-MSC and its Influence on Their Application to Clinical Procedures
title_sort	banking of at-msc and its influence on their application to clinical procedures
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/69fcf72aa9d34a73b3812723737a733f
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Banking of AT-MSC and its Influence on Their Application to Clinical Procedures

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