Thermoresponsive gels containing gold nanoparticles as smart antibacterial and wound healing agents

Abstract Thermoresponsive gels containing gold nanoparticles (AuNPs) were prepared using Pluronic®127 alone (F1) and with hydroxypropyl methylcellulose (F2) at ratios of 15% w/w and 15:1% w/w, respectively. AuNPs were evaluated for particle size, zeta-potential, polydispersity index (PDI), morpholog...

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Autores principales:	Mona G. Arafa, Reham F. El-Kased, M. M. Elmazar
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Thermoresponsive Gel Gold Nanoparticles Gelation Temperature Transdermal Drug Delivery Systems Burn Wound Infection Medicine R Science Q
Acceso en línea:	https://doaj.org/article/69fffae7a51b421ebf865ef2cc4ab2cf
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spelling	oai:doaj.org-article:69fffae7a51b421ebf865ef2cc4ab2cf2021-12-02T15:08:17ZThermoresponsive gels containing gold nanoparticles as smart antibacterial and wound healing agents10.1038/s41598-018-31895-42045-2322https://doaj.org/article/69fffae7a51b421ebf865ef2cc4ab2cf2018-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-31895-4https://doaj.org/toc/2045-2322Abstract Thermoresponsive gels containing gold nanoparticles (AuNPs) were prepared using Pluronic®127 alone (F1) and with hydroxypropyl methylcellulose (F2) at ratios of 15% w/w and 15:1% w/w, respectively. AuNPs were evaluated for particle size, zeta-potential, polydispersity index (PDI), morphology and XRD pattern. AuNP-containing thermoresponsive gels were investigated for their gelation temperature, gel strength, bio-adhesive force, viscosity, drug content, in vitro release and ex-vivo permeation, in addition to in vitro antibacterial activity against bacteria found in burn infections, Staphylococcus aureus. In vivo burn healing and antibacterial activities were also investigated and compared with those of a commercial product using burn-induced infected wounds in mice. Spherical AuNPs sized 28.9–37.65 nm displayed a surface plasmon resonance band at 522 nm, a PDI of 0.461, and a zeta potential of 34.8 mV with a negative surface charge. F1 and F2 showed gelation temperatures of 37.2 °C and 32.3 °C, bio-adhesive forces of 2.45 ± 0.52 and 4.76 ± 0.84 dyne/cm2, viscosities of 10,165 ± 1.54 and 14,213 ± 2.31 cP, and gel strengths between 7.4 and 10.3 sec, respectively. The in vitro release values of F1 and F2 were 100% and 98.03% after 6 h, with permeation flux values of (J1) 0.2974 ± 2.85 and (J2) 0.2649 ± 1.43 (µg/cm2·h), respectively. The formulations showed antibacterial activity with the highest values for wound healing properties, as shown in vivo and by histopathological studies. This study demonstrates that a smart AuNPs thermoresponsive gel was successful as an antibacterial and wound healing transdermal drug delivery system.Mona G. ArafaReham F. El-KasedM. M. ElmazarNature PortfolioarticleThermoresponsive GelGold NanoparticlesGelation TemperatureTransdermal Drug Delivery SystemsBurn Wound InfectionMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-16 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Thermoresponsive Gel Gold Nanoparticles Gelation Temperature Transdermal Drug Delivery Systems Burn Wound Infection Medicine R Science Q
spellingShingle	Thermoresponsive Gel Gold Nanoparticles Gelation Temperature Transdermal Drug Delivery Systems Burn Wound Infection Medicine R Science Q Mona G. Arafa Reham F. El-Kased M. M. Elmazar Thermoresponsive gels containing gold nanoparticles as smart antibacterial and wound healing agents
description	Abstract Thermoresponsive gels containing gold nanoparticles (AuNPs) were prepared using Pluronic®127 alone (F1) and with hydroxypropyl methylcellulose (F2) at ratios of 15% w/w and 15:1% w/w, respectively. AuNPs were evaluated for particle size, zeta-potential, polydispersity index (PDI), morphology and XRD pattern. AuNP-containing thermoresponsive gels were investigated for their gelation temperature, gel strength, bio-adhesive force, viscosity, drug content, in vitro release and ex-vivo permeation, in addition to in vitro antibacterial activity against bacteria found in burn infections, Staphylococcus aureus. In vivo burn healing and antibacterial activities were also investigated and compared with those of a commercial product using burn-induced infected wounds in mice. Spherical AuNPs sized 28.9–37.65 nm displayed a surface plasmon resonance band at 522 nm, a PDI of 0.461, and a zeta potential of 34.8 mV with a negative surface charge. F1 and F2 showed gelation temperatures of 37.2 °C and 32.3 °C, bio-adhesive forces of 2.45 ± 0.52 and 4.76 ± 0.84 dyne/cm2, viscosities of 10,165 ± 1.54 and 14,213 ± 2.31 cP, and gel strengths between 7.4 and 10.3 sec, respectively. The in vitro release values of F1 and F2 were 100% and 98.03% after 6 h, with permeation flux values of (J1) 0.2974 ± 2.85 and (J2) 0.2649 ± 1.43 (µg/cm2·h), respectively. The formulations showed antibacterial activity with the highest values for wound healing properties, as shown in vivo and by histopathological studies. This study demonstrates that a smart AuNPs thermoresponsive gel was successful as an antibacterial and wound healing transdermal drug delivery system.
format	article
author	Mona G. Arafa Reham F. El-Kased M. M. Elmazar
author_facet	Mona G. Arafa Reham F. El-Kased M. M. Elmazar
author_sort	Mona G. Arafa
title	Thermoresponsive gels containing gold nanoparticles as smart antibacterial and wound healing agents
title_short	Thermoresponsive gels containing gold nanoparticles as smart antibacterial and wound healing agents
title_full	Thermoresponsive gels containing gold nanoparticles as smart antibacterial and wound healing agents
title_fullStr	Thermoresponsive gels containing gold nanoparticles as smart antibacterial and wound healing agents
title_full_unstemmed	Thermoresponsive gels containing gold nanoparticles as smart antibacterial and wound healing agents
title_sort	thermoresponsive gels containing gold nanoparticles as smart antibacterial and wound healing agents
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/69fffae7a51b421ebf865ef2cc4ab2cf
work_keys_str_mv	AT monagarafa thermoresponsivegelscontaininggoldnanoparticlesassmartantibacterialandwoundhealingagents AT rehamfelkased thermoresponsivegelscontaininggoldnanoparticlesassmartantibacterialandwoundhealingagents AT mmelmazar thermoresponsivegelscontaininggoldnanoparticlesassmartantibacterialandwoundhealingagents
_version_	1718388190348312576

Thermoresponsive gels containing gold nanoparticles as smart antibacterial and wound healing agents

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